Research Article

Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye

Volume: 9 Number: 2 November 9, 2026
EN TR

Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye

Abstract

Bee pollen is a nutritionally valuable apicultural product rich in proteins, phenolic compounds, flavonoids, and other bioactive constituents, the composition of which is strongly influenced by botanical and geographical origin. This study evaluated the physicochemical properties, antioxidant characteristics, and microbiological quality of bee pollen collected from three districts (Geyve, Hendek, and Karasu) of Sakarya Province, Türkiye. Physicochemical properties (moisture, ash and protein), total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity (DPPH and FRAP), and microbiological quality were evaluated. Microbiological analyses included the enumeration of total aerobic mesophilic bacteria (TAMB), total yeasts and molds, Escherichia coli, and Staphylococcus aureus. Significant differences (p < 0.05) were observed among the sampling locations for physicochemical and antioxidant parameters, as well as for total aerobic mesophilic bacteria and yeast–mold counts. Bee pollen from Geyve exhibited the highest protein, total phenolic and flavonoid contents, together with the greatest antioxidant capacity, whereas Karasu samples showed the highest microbial counts. Neither E. coli nor S. aureus was detected in any sample, supporting the satisfactory microbiological quality of the analyzed bee pollen. The analyzed bee pollen samples exhibited favorable physicochemical and antioxidant characteristics and satisfactory microbiological quality with respect to the investigated parameters. Furthermore, this study provides the first comprehensive characterization of bee pollen produced in Sakarya Province and contributes valuable regional data to the existing literature on Turkish bee pollen.

Keywords

Bee pollen, Antioxidant activity, Phenolic compounds, Microbiological quality

Ethical Statement

This study did not involve any human participants or animals. Therefore, ethics committee approval was not required.

References

  1. Adaškevičiūtė, V., Kaškonienė, V., Kaškonas, P., Barčauskaitė, K., & Maruška, A. (2019). Comparison of physicochemical properties of bee pollen with other bee products. Biomolecules, 9(12), Article 819.
  2. Alippi, A. M., Fernández, L. A., & López, A. C. (2021). Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina. Journal of Apicultural Research, 60(5), 730–737.
  3. Almeida-Muradian, L. B., Pamplona, L. C., Coimbra, S., & Barth, O. M. (2005). Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis, 18(1), 105–111.
  4. Asmae, E. G., Nawal, E. M., Bakour, M., & Lyoussi, B. (2021). Moroccan monofloral bee pollen: Botanical origin, physicochemical characterization, and antioxidant activities. Journal of Food Quality, 2021(1), Article 8877266.
  5. Asoutis Didaras, N., Dimitriou, T., Daskou, M., Karatasou, K., & Mossialos, D. (2022). In vitro assessment of the antiviral activity of Greek bee bread and bee-collected pollen against enterovirus D68. Journal of Microbiology, Biotechnology and Food Sciences, 11(5), Article e4859.
  6. Association of Official Analytical Chemists. (1990). Official methods of analysis (15th ed., Method 976.05).
  7. Association of Official Analytical Chemists. (2000a). Official methods of analysis (17th ed., Method 969.38, Moisture in honey, Chapter 44, p. 23). AOAC International.
  8. Association of Official Analytical Chemists. (2000b). Official methods of analysis (17th ed., Method 923.03, Ash in flour, Chapter 32). AOAC International.
  9. Aylanc, V., Falcão, S. I., Ertosun, S., & Vilas-Boas, M. (2021). From the hive to the table: Nutritional value, digestibility and bioavailability of the dietary phytochemicals present in bee pollen and bee bread. Trends in Food Science & Technology, 109, 464–481.
  10. Aylanc, V., Falcão, S. I., Ertosun, S., Vilas-Boas, M., Dias, L. G., & Carvalho, A. M. (2023). Evaluation of antioxidant and anticancer activity of mono- and polyfloral Moroccan bee pollen by characterizing phenolic and volatile compounds. Molecules, 28(3), Article 835.
APA
Sönmez, E. (2026). Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye. Journal of Apitherapy and Nature, 9(2), 319-333. https://doi.org/10.35206/jan.2016067
AMA
1.Sönmez E. Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye. J.Apit.Nat. 2026;9(2):319-333. doi:10.35206/jan.2016067
Chicago
Sönmez, Emine. 2026. “Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye”. Journal of Apitherapy and Nature 9 (2): 319-33. https://doi.org/10.35206/jan.2016067.
EndNote
Sönmez E (November 1, 2026) Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye. Journal of Apitherapy and Nature 9 2 319–333.
IEEE
[1]E. Sönmez, “Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye”, J.Apit.Nat., vol. 9, no. 2, pp. 319–333, Nov. 2026, doi: 10.35206/jan.2016067.
ISNAD
Sönmez, Emine. “Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye”. Journal of Apitherapy and Nature 9/2 (November 1, 2026): 319-333. https://doi.org/10.35206/jan.2016067.
JAMA
1.Sönmez E. Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye. J.Apit.Nat. 2026;9:319–333.
MLA
Sönmez, Emine. “Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye”. Journal of Apitherapy and Nature, vol. 9, no. 2, Nov. 2026, pp. 319-33, doi:10.35206/jan.2016067.
Vancouver
1.Emine Sönmez. Physicochemical Characteristics, Antioxidant Properties, and Microbiological Quality of Bee Pollen Collected from Three Distinct Locations in Sakarya Province, Türkiye. J.Apit.Nat. 2026 Nov. 1;9(2):319-33. doi:10.35206/jan.2016067