Buzdolabında (0-4°C) Depolama Sırasında Vakumla Paketlenmiş Levrek (Dicentrarchus labrax) Kalite Parametrelerinin İzlenmesi ve Elektronik Burun Etkinliğinin Değerlendirilmesi

Yıl 2025, Cilt: 21 Sayı: 4, 266 - 280

Ömer Alper Erdem , Şebnem Tolasa Yılmaz , Şükran Çaklı , Arzu Burcu Yavuz

https://doi.org/10.22392/actaquatr.1637599

Öz

Elektronik burun (e-burun) kullanımının, 18 günlük depolama (0-4°C) süresince vakumlu paketlenmiş, temizlenmiş ve pulları alınmış levrek (Dicentrarchus labrax) üzerinde etkinliği incelenmiştir. Bu çalışmada, mikrobiyolojik (toplam aerobik mezofilik bakteri, psikrofilik bakteri ve aerobik bakteri sayısı), kimyasal (TVB-N, TMA, TBARS) ve duyusal kalite de belirlendi. Toplam aerobik mezofilik ve psikrofilik bakteri sayıları, vakumlu paketlenmiş ve içi temizlenmiş levreklerin depolanması sırasında arttı. Toplam aerobik mezofilik bakteriler, psikrofilik bakterilerin sayısı 18. günde raf ömrü sınırını (7 log cfu/g) aşmıştır. Depolama sürecinin 18. gününün tamamlanmasının ardından; TVB-N, TBARS, TMA-N değerleri sırasıyla 22,42±2,87 mg/100 g, 0,37±0,12 µmol/100g, 4,12±0,41 mg/100 g olarak belirlenmiştir. Duyusal puan, depolamanın 18. gününde 14.43±0.14 olarak belirlendi. Bu çalışmanın bulguları, 4°C'de saklanan vakumlu ambalajlı levreklerin mikrobiyolojik verilerinin, raf ömrünün saklama süresinin 15. günü olduğunu göstermektedir. Elektronik burun ölçümlerinde, balığın tazeliğinin devam ettiği varsayılan 1., 4., 6. ve 8. günlerdeki sensör sonuçları birbirine yakın değerler verirken, 8. gün dağılımları diğerlerine göre daha dağınık olup, bu durum koku değişikliklerinin başladığının bir göstergesi olarak kabul edilmiştir. Depolamanın 11., 13. ve 15. günlerine ait değerler birbirine yakın olup bazen aynı dağılımı vermiştir. Sonuçlar, elektronik burun teknolojisinin balık tazeliğini izleme açısından potansiyel bir araç olduğunu ve geleneksel duyusal analizlerle birlikte kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Dicentrarchus labrax , balık kalitesi , vakum paket , e-burun.

Proje Numarası

12-BIL-029

Monitoring of Quality Parameters in Vacuum-Packed Sea Bass (Dicentrarchus labrax) During Refrigerated Storage at (0-4°C) and Evaluation of Electronic Nose Effectiveness

Öz

The effectiveness of electronic nose (e-nose) on vacuum packed, gutted and descaled sea bass (Dicentrarchus labrax) was investigated during 18 days in refrigeration (0-4°C). Microbiological (total aerobic mesophilic bacteria, psychrophilic bacteria and aerobic bacteria counts) chemical (TVB-N, TMA, TBARS) and sensory quality were also determined in this study. Total aerobic mesophilic and psychrophilic bacteria counts increased during the storage period of vacuum packed and gutted European sea bass . Total aerobic mesophilic bacteria and psychrophilic bacteria exceeded the shelf life limit (7 log cfu/g) on day 18. Upon the completion of the 18th day of the storage period; TVB-N, TBARS, TMA-N values were determined as 22.42±2.87 mg/100 g, 0.37±0.12 µmol/100g, 4.12±0.41 mg/100 g, respectively. The sensory score was determined as 14.43±0.14 on the 18th days of storage. The findings of this study reveal that the microbiological data of the vacuum-packed sea bass stored at 4°C indicate that the shelf life was 15 days of the storage period. In the electronic nose measurements, the sensor results on days 1st, 4th, 6th and 8th days, which are assumed to continue the freshness of the fish, gave close values, while the 8th day distributions were more scattered than the others, and this was accepted as an indication that odour changes had begun. The values for the 11th, 13th and 15th days of storage were close to each other and sometimes gave the same distribution. The results demonstrate that electronic nose technology is a potential tool for monitoring fish freshness and can be used in conjunction with traditional sensory analyses.

Anahtar Kelimeler

Dicentrarchus labrax , fish quality , vacuum-pack , e-nose

Etik Beyan

Local Ethics Committee Approval was not obtained because experimental animals were harvested before purchase in the present study.

Destekleyen Kurum

Present study was supported by Ege University Scientific Research Projects Coordination (Grant number: 12-BIL-029).

Proje Numarası

12-BIL-029

Teşekkür

This paper is based on the presentation at the 1st International Fisheries Symposium in Northern Cyprus (24-27 March 2013, Girne, Northern Cyprus). Arzu Burcu Yavuz, who is a member of the working team, is eligible for the TUBITAK 2211-C Priority Fields Doctorate Scholarship Program.

Kaynakça

• Alasalvar, C., Taylor, K. D. A., Öksüz, A., Garthwaite, T., Alexis, M. N., & Grigorakis, K. (2001). Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods. Food chemistry, 72(1), 33-40.
• Al-Hooti, H. S., Al-Bulushi, I. M., Al-Attabi, Z. H., Rahman, M. S., Al-Subhi, L. K., & Al-Habsi, N. A. (2024). Efficiency of electronic nose in detecting the microbial spoilage of fresh sardines (Sardinella longiceps). Foods, 13(3), 428.
• Amari, K., Díaz‐Vivancos, P., Pallás, V., Sánchez-Pina, M. A., & Hernández, J. A. (2007). Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds. Physiologia Plantarum, 131(2), 302-310.
• Çakli, S., Kilinc, B., Cadun, A., Dincer, T., & Tolasa, S. (2007). Quality differences of whole ungutted sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) while stored in ice. Food Control, 18(5), 391-397.
• Carrascosa, C., Millán, R., Saavedra, P., Jaber, J.R., Montenegro, T., Raposo, A., Esteban Pérez, E. & Sanjuán, E. (2014). Predictive models for bacterial growth in sea bass (Dicentrarchus labrax) stored in ice. International Journal of Food Science and Technology, 49, 354–363.
• Castro, P., Penedo Padrón, J. C., Caballero Cansino, M. J., Sanjuán Velázquez, E., & Millán De Larriva, R. (2006). Total volatile base nitrogen and its use to assess freshness in European sea bass stored in ice. Food Control, 17(4), 245–248.
• Chang, K. L. B., Chang, J., Shiau, C.-Y., & Pan, B. S. (1998). Biochemical, microbiological, and sensory changes of sea bass (Lateolabrax japonicus) under partial freezing and refrigerated storage. Journal of Agricultural and Food Chemistry, 46(2), 682-686.
• Connell, J. J. (1995). Control of fish quality (4th ed.). London, UK: Fishing News (Books) Limited.
• Debevere, J., & Boskou, G. (1996). Effect of modified atmosphere packaging on the TVB/TMA-producing microflora of cod fillets. International Journal of Food Microbiology, 31(1-3), 221-229.
• Erdem, Ö. A. (2016). Quality monitoring using electronic nose and machine vision on some cultured fish species at refrigerator storage (PhD. Thesis in Turkish). Ege University Graduate School of Natural and Applied Sciences, Türkiye.
• Fuentes-Amaya, L. F., Munyard, S., Fernandez-Piquer, J., & Howieson, J. (2016). Sensory, microbiological and chemical changes in vacuum-packaged blue spotted emperor (Lethrinus sp), saddletail snapper (Lutjanus malabaricus), crimson snapper (Lutjanus erythropterus), barramundi (Lates calcarifer), and Atlantic salmon (Salmo salar) fillets stored at 4°C. Food Science & Nutrition, 4(3), 479–489.
• Gardner, J. W., & Bartlett, P. N. (1999). Odour sensors. In J. W. Gardner & P. N. Bartlett (Eds.), Electronic Noses: Principles and Applications (pp.67-116). Oxford University Press.
• Ghaly, A. E., Dave, D., Budge, S. M., & Brooks, M. S. (2010). Fish spoilage mechanisms and preservation techniques: review. American Journal of Applied Sciences, 7(7), 859-877.
• Gliszczyńska-Świgło, A., & Chmielewski, J. (2017). Electronic nose as a tool for monitoring the authenticity of food. A review. Food Analytical Methods, 10, 1800-1816.
• Gram, L., & Huss, H. H. (1996). Microbiological spoilage of fish and fish products. International Journal of Food Microbiology, 33(1), 121-137.
• Grassi, S., Benedetti, S., Opizzio, M., Nardo, E. d., & Buratti, S. (2019). Meat and fish freshness assessment by a portable and simplified electronic nose system (Mastersense). Sensors, 19(14), 3225.
• Houicher, A., Küley, E., Bendeddouche, B., & Özoğul, F. (2013). Effect of Mentha spicata L. and Artemisia campestris extracts on the shelf life and quality of vacuum-packed refrigerated sardine (Sardina pilchardus) fillets. Journal of Food Protection, 76(10), 1719-1725.
• Hsiao, H., & Chang, J. (2016). Developing a microbial time-temperature indicator to monitor total volatile basic nitrogen change in chilled vacuum-packed grouper fillets. Journal of Food Processing and Preservation, 41(5), e13158.
• Huidobro, A., Mendes, R., & Nunes, M. (2001). Slaughtering of gilthead seabream (Sparus aurata) in liquid ice: influence on fish quality. European Food Research and Technology, 213, 267-272.
• Iacumin, L., Jayasinghe, A. S., Pellegrini, M., & Comi, G. (2022). Evaluation of different techniques, including modified atmosphere, under vacuum packaging, washing, and Latilactobacillus sakei as a bioprotective agent, to increase the shelf-life of fresh gutted sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) stored at 6 ± 2 °C. Biology, 11(2), 217.
• ICMSF, (1983). Métodos recomendados para el análisis microbiológico en alimentos, In: Microorganismos de los alimentos, I. Técnicas de análisis microbiológicos, 2da, Ed. Acribia, Zaragoza, Espanã, 105-280pp.
• Incegül, Y., Ozkan, G., Incegül, A., & Taşdelen, K. (2022). Use of electronic nose metal oxide semiconductor sensors in food analysis. Akademik Gıda, 20(4), 454-473. (in Turkish)
• Kızıltan, S. (2024). Turkish seafood: a rising star in the global market. 52nd WEFTA Annual Meeting, Cesme, Izmir, Turkiye, 14-18 October 2024.
• Kritikos, A., Aska, I., Εkonomou, S. Ι., Mallouchos, A., Parlapani, F. F., Haroutounian, S. A., & Boziaris, I. S. (2020). Volatilome of chill-stored European sea bass (Dicentrarchus labrax) fillets and Atlantic salmon (Salmo salar) slices under modified atmosphere packaging. Molecules, 25(8), 1981.
• Kyrana, V. R., & Lougovois, V. P. (2002). Sensory, chemical and microbiological assessment of farm-raised European sea bass (Dicentrarchus labrax) stored in melting ice. International Journal of Food Science and Technology, 37, 319–328.
• Lan, W., Shao, Z., Lang, A., Xie, J. (2024). Effects of slightly acidic electrolyzed water combined with ԑ-polylysine-chitooligosaccharide Maillard reaction products treatment on the quality of vacuum packaged sea bass (Lateolabrax japonicus). International Journal of Biological Macromolecules, 260, 129554.
• Lan, W., Zhao, Y., Liu, J., Xie, J. (2022). Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage. Journal of Food Protection, 85, 5, 803–814.
• Lemon, D.W. (1975). An improved TBA test for rancidity. In New Series Circular, no 51; Oceans Canada, Halifax Laboratory: Halifax, Noca Scotia.
• Li, P., Ren, Z., Shao, K., Tan, H., & Niu, Z. (2019). Research on distinguishing fish meal quality using different characteristic parameters based on electronic nose technology. Sensors, 19(9), 2146.
• Madhubhashini, M. N., Liyanage, C. P., Alahakoon, A. U., & Liyanage, R. P. (2023). Current applications and future trends of artificial senses in fish freshness determination: A review. Journal of Food Science, 89(1), 33-50.
• Malle, P., & Poumeyrol, M. (1989). A new chemical criterion for the quality control of fish: trimethylamine/total volatile basic nitrogen (%). Journal of Food Protection, 52(6), 419-423.
• Mediterranean Sea bass (Dicentrarchus labrax). Food Microbiology, 21, 549–557.
• Moretti, V., Vasconi, M., Caprino, F., & Bellagamba, F. (2016). Fatty acid profiles and volatile compounds formation during processing and ripening of a traditional salted dry fish product. Journal of Food Processing and Preservation, 41(5), e13133.
• Nguyen, M. V., Karnue, S., & Kakooza, D. (2023). Effect of packaging method and storage temperature on the sensory quality and lipid stability of fresh snakehead fish (Channa striata) fillets. Food Science and Technology, 43, e116222.
• Paleologos, E.K., Savvaidis, I.N., Kontominas, M.G. (2004). Biogenic amines formation and its relation to microbiological and sensory attributes in ice-stored whole, gutted and filleted
• Parlapani, F.F., Serkos A. Haroutounian, S.A., Nychas, G-J. E., Boziaris, I.S. (2015). Microbiological spoilage and volatiles production of gutted European sea bass stored under air and commercial modified atmosphere package at 2 °C. Food Microbiology, 50, 44-53.
• Phuhongsung, P., Zhang, M., Bhandari, B., & Gao, Z. (2017). Effect of vacuum storage on the freshness of grass carp (Ctenopharyngodon idella) fillet based on normal and electronic sensory measurement. Journal of Food Processing and Preservation, 42(2), e13418.
• Poli, B. M., Parisi, G., Zampacavallo, G., Iurzan, F., Mecatti, M., Lupi, P., & Franci, O. (2001). Quality outline of European sea bass (Dicentrarchus labrax) reared in Italy: Shelf life, edible yield, nutritional and dietetic traits. Aquaculture, 202(3), 303–315.
• Şahin, M.E. (2008). Determination of microorganisms by means of electronic nose and principle component analysis (MSc Thesis in Turkish). Dumlupınar University Graduate School of Natural and Applied Sciences, Türkiye.
• Senapati, S. R., Kumar, G. P., Singh, C. B., Xavier, K. M., Chouksey, M. K., Nayak, B. B., & Balange, A. K. (2017). Melanosis and quality attributes of chill stored farm raised whiteleg shrimp (Litopenaeus vannamei). Journal of Applied and Natural Science, 9(1), 626-631.
• Souza, B. W., Cerqueira, M. A., Ruiz, H. A., Martins, J. T., Casariego, A., Teixeira, J. A., & Vicente, A. A. (2010). Effect of chitosan-based coatings on the shelf life of salmon (Salmo salar). Journal of Agricultural and Food Chemistry, 58(21), 11456-11462.
• Wilson, A. D., Oberle, C. S., & Oberle, D. F. (2013). Detection of off-flavor in catfish using a conducting polymer electronic-nose technology. Sensors, 13(12), 15968-15984.
• Wu, K., Debliquy, M., & Zhang, C. (2022). Metal-oxide-semiconductor resistive gas sensors for fish freshness detection. Comprehensive Reviews in Food Science and Food Safety, 22(2), 913-945.
• Zaukuu, J. Z., Bázár, G., Gillay, Z., & Kovács, Z. (2019). Emerging trends of advanced sensor based instruments for meat, poultry and fish quality- a review. Critical Reviews in Food Science and Nutrition, 60(20), 3443-3460.