Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates

Yıl 2019, , 444 - 449, 31.12.2019

Dilek Düyüncü Seyhan Ulusoy

https://doi.org/10.24323/akademik-gida.667250

Cited By: 1

Öz

Currently, leafy green salads are often consumed because they are considered as practical and healthy. However, during their preparation, both inadequate washing and contact with non-hygienic surfaces may increase their microbial load. This may cause several health problems for individuals consuming salads. The overuse of antibiotics has led to the emergence of multidrug resistant bacteria, including foodborne pathogens. Biofilm production ability of these pathogenic bacteria makes it difficult to treat infections caused by these pathogens. The aim of this study is to isolate and identify P. fluorescens from leafy green salads collected from different restaurants. A total of 72 isolates were isolated from leafy green salads, and 29 of these isolates were identified by PCR as Pseudomonas and 9 of them identified as P. fluorescens. All P. fluorescens isolates were resistant to ampicillin, amoxicillin, cefuroxime, ceftazidime and ceftriaxone antibiotics. The results of this study showed that additional attention for the hygiene conditions is needed during the preparation and storage stages of leafy green salads.

Anahtar Kelimeler

Leafy green salad, Pseudomonas fluorescens, Antibiotic resistance

Destekleyen Kurum

Süleyman Demirel University Scientific Research Projects Management Unit

Proje Numarası

3955-YL1-14

Teşekkür

We would like to thank the Head of Scientific Research Projects Management Unit of Süleyman Demirel University for supporting this study with the project number 3955-YL1-14.

Yeşil Salatalardan Pseudomonas fluorescens İzolasyonu ve İzolatların Antibiyotik Duyarlılıkları

Öz

Günümüzde yeşil salatalar genellikle pratik ve sağlıklı olduklarını düşünerek tüketilmektedir. Ancak salataların hazırlanması sırasında, salata malzemelerinin yetersiz yıkanması ve hijyenik olmayan yüzeylerle teması, salataların mikrobiyal yükünü arttırmaktadır. Bu durum salataları tüketen bireyler için sağlık sorunlarına neden olabilir. Antibiyotiklerin aşırı kullanımı, gıda kaynaklı patojenler dahil olmak üzere çoklu ilaç direncine sahip bakterilerin ortaya çıkmasına neden olmuştur. Bu patojen bakterilerin biyofilm üretme özelliklerinin olması, bu patojenlerin sebep oldukları enfeksiyonların tedavisini zorlaştırmaktadır. Bu çalışmanın amacı, farklı restoranlardan temin edilen yeşil salatalardan P. fluorescens izolasyonu ve tanımlanmasıdır. Yeşil salatalardan toplam 72 izolat izole edilmiş ve bu izolatların 29'u PZR ile Pseudomonas olarak ve 9'u P. fluorescens olarak tanımlanmıştır. Tüm P. fluorescens izolatlarının ampisilin, amoksisilin, sefuroksim, seftazidime ve seftriakson antibiyotiklerine dirençli olduğu bulunmuştur. Bu çalışmanın sonuçları, yeşil salataların hazırlanması ve depolanması sırasında hijyen koşullarına daha fazla dikkat edilmesi gerektiğini göstermektedir.

Anahtar Kelimeler

Yeşil salata, Pseudomonas fluorescens, Antibiyotik direnci

Proje Numarası

3955-YL1-14

Kaynakça

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