Sweetener, Antioxidant and Antimicrobial Properties of Stevia rebaudiana

Yıl 2018, Cilt: 16 Sayı: 4, 431 - 438, 31.12.2018

Şeyda Karagöz Aslıhan Demirdöven

https://doi.org/10.24323/akademik-gida.505522

Öz

Stevia rebaudiana, also known as sugar grass, has been used by millions
of people for years because of its sweetening and therapeutic properties. In a
number of studies conducted, researchers also reported various properties of
stevia plant which can be used in food industry. Stevia rebaudiana leaves contain all nutrients at different
concentrations but steviol glycosides, that give 100 to 300 times the sweetness
of sucrose, are the most important compounds in this plant. It is also stated
that there are flavonoids and phenolic compounds that give antimicrobial and
antioxidant properties to food in stevia leaves. This shows that stevia can be
used for different purposes as well as a sweetener. The purpose of this study
was to review the sweetener, antioxidant and antimicrobial properties of Stevia rebaudiana.

Anahtar Kelimeler

Stevia rebaudiana, Antimicrobial, Antioxidant, Sweetener

Stevia rebaudiana Bitkisinin Tatlandırıcı, Antioksidan ve Antimikrobiyal Özellikleri

Öz

Şeker otu olarak da bilinen Stevia rebaudiana, tatlandırıcı ve
tedavi edici özelliklerinden dolayı milyonlarca insan tarafından yıllardır
kullanılmaktadır. Bununla birlikte yapılan çalışmalarda araştırmacılar stevia
bitkisinin gıda sektöründe kullanılması mümkün olan çeşitli özelliklerini de
rapor etmişlerdir. Stevia rebaudiana
yaprakları bütün besin maddelerini az da olsa içermektedir. Ancak bu bitkiye
sakkarozdan 100 ile 300 kat tatlılık özelliği kazandıran en önemli bileşikler
steviol glikozitlerdir. Ayrıca stevia yapraklarında gıdaya antimikrobiyal ve
antioksidan özelliği kazandıran flavonoitler ve fenolik bileşiklerin olduğu da
belirtilmiştir. Bu durum stevianın tatlandırıcı olarak kullanılmasının yanı
sıra farklı amaçlarla da kullanılabileceğini göstermektedir. Bu çalışmada, Stevia rebaudiana bitkisinin
tatlandırıcı, antioksidan ve antimikrobiyal özelliklerinin derlenmesi
amaçlanmıştır.

Anahtar Kelimeler

Stevia rebaudiana, Antimikrobiyal, Antioksidan, Tatlandırıcı

Kaynakça

• [1] Kesici-Güler, H., Dönmez, İ.E., Alay-Aksoy, S. (2015). Tıbbi ve aromatik bitkilerin antibakteriyel aktivitesi ve tekstil sektöründe kullanımı. Süleyman Demirel Üniversitesi Fen Dergisi, 10(2), 27-34.
• [2] Faydaoğlu, E., Sürücüoğlu, M.S. (2013). Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. EÜFBED 6(2): 233-265.
• [3] Sarić, G., Marković, K., Vukičević, D., Lež, E., Hruškar, M., Vahčić, N. (2013). Changes of antioxidant activity in honey after heat treatment. Czech Journal of Food Sciences, 31(6), 601–606.
• [4] Bajčan, D., Tomáš, J., Uhlířová, G., Árvay, J., Trebichalský, P., Stanovič, R., Šimanský, V. (2013). Antioxidant potential of spinach, peas and sweet corn in relation to freezing period. Czech Journal of Food Sciences, 31(6), 613–618.
• [5] Kobus-Moryson, M., Gramza-Michałowska, A. (2015). Directions on the use of stevia leaves (Stevia rebauidana) as an additive in food products. Acta Sci. Pol. Technol. Aliment., 14(1), 5–13.
• [6] İnanç, A.L., Çınar, İ. (2009). Alternatif doğal tatlandırıcı: Stevya. Gıda, 34(6), 411-415.
• [7] Gantait, S., Das, A., Mandal, N. (2015). Stevia: A comprehensive review on ethnopharmacological properties and in vitro regeneration june. Sugar Technology, 17(2), 95-106.
• [8] Boileau, A., Fry, J., Murray, R. (2012). A new calorie-free sugar substitute from the leaf of the stevia plant arrives in the UK. Nutr. Bull., 37(1), 47-50.
• [9] Yang, Y.H., Huang, S.Z., Han, Y.L., Yuan, H.Y., Gu, C.S., Zhao, Y.H. (2014). Base substitution mutations in uridinediphosphate-dependent glycosyltransferase 76g1 gene of Stevia rebaudiana causes the low levels of rebaudioside a mutations in ugt76g1, a key gene of steviol glycosides synthesis. Plant Physiol. Bioch., 80, 220-225.
• [10] Yadav, A.K., Singh, S., Dhyani, D., Ahuja, P.S. (2011). A review on the improvement of stevia [Stevia rebaudiana (bertoni)]. Can. J. Plant Sci., 91(1), 1-27.
• [11] Periche, A., Castelló, M.L., Heredia, A., Escriche, I. (2015). Influence of drying method on steviol glycosides and antioxidants in Stevia rebaudiana leaves. Food Chemistry, 172, 1–6.
• [12] Balkır, P. (2016). Stevia; fonksiyonel özellikleri ve gıdalarda kullanım olanakları. Gıda, 41(6), 435-442.
• [13] Barba, F.J., Esteve, MJ., Frígola, A. (2014). Bioactive components from leaf vegetable products. Stud. Nat. Prod. Chem., 41(11), 321–346.
• [14] Belda-Galbis, C.M., Pina-Pérez, M.C., Espinosa, J., Marco-Celdrán, A., Martínez, A., Rodrigo, D. (2014). Use of the modified gompertz equation to assess the Stevia rebaudiana bertoni antilisterial kinetics. Food Microbiology, 38, 56–61.
• [15] Criado, M.N., Barba, F.J., Frígola, A., Rodrigo, D. (2014). Effect of Stevia rebaudiana on oxidative enzyme activity and its correlation with antioxidant capacity and bioactive compounds. Food Bioprocess Technology, 7(5), 1518–1525.
• [16] Muanda, F.N., Soulimani, R., Diop, B., Dicko, A. (2011). Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana bertoni leaves. LWT-Food Science and Technology, 44(9), 1865–1872.
• [17] Barba, F.J., Grimi, N., Vorobiev, E. (2015). Evaluating the potential of cell disruption technologies for green selective extraction of antioxidant compounds from Stevia rebaudiana bertoni leaves. Journal of Food Engineering, 149, 222–228.
• [18] Hajihashemi, S., Geuns, J.M.C. (2014). Radical scavenging activity of steviol glycosides, steviol glucuronide, hydroxytyrosol, metformin, aspirin and leaf extract of Stevia rebaudiana. Free Radicals Antioxidant, 3, 34-41.
• [19] Kunyanga, C.N., Imungi, J.K., Okoth, M.W., Biesalski, H.K., Vadivel, V. (2012). Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT-Food Science and Technology, 45, 269-276.
• [20] Uçar, E., Odabaş-Köse, E., Özyiğit, Y., Turgut, K. (2015). Bazı tıbbi ve aromatik bitkilerde esansiyel yağların antimikrobiyal aktivitelerinin belirlenmesi. SDÜ Ziraat Fakültesi Dergisi, 10(2), 118-124.
• [21] Kim, I.S., Yang, M., Lee, O.H., Kang, S.N. (2011). The antioxidant activity and the bioactive compounds content of Stevia rebaudiana water extracts. Food Science and Technology, 44, 1328–1332.
• [22] Hajihashemi, S., Ehsanpour, A.A. (2014). Antioxidant response of Stevia rebaudiana b. to polyethylene glycol and paclobutrazol treatments under ın vitro culture. Appl. Biochem. Biotechnol., 172, 4038–4052.
• [23] Chrıstakı, E., Bonos, E., Gıannenas, I., Karatzıa, M.A., Florou-Paneri, P. (2013). Stevia rebaudiana as a novel source of food additives. J. Food Nutr. Res., 52(4), 195–202.
• [24] Braz de Oliveira, A.J., Correia Goncalves, R.A., Cantuaria Chierrito, T.P., Muller dos Santos, M., Mera de Souza, L., Gorin, P.A.J., Lanzi Sassaki, G., Lacomini, M. (2011). Structure and degree of polymerisation of fructooligosaccharides present in roots and leaves of Stevia rebaudiana (Bert.) bertoni. Food Chemistry, 129, 305–311.
• [25] Ghosh, S., Subudhi, E., Nayak, S. (2008). Antimicrobial assay of Stevia rebaudiana bertoni leaf extracts against 10 pathogens. IJIB, 2, 27–31.
• [26] Lemus-Mondaca, R., Vega-Gálvez, A., Zura-Bravo, L., Ah-Hen, K. (2012). Stevia rebaudiana bertoni, source of a high-potency natural sweetener: a comprehensive review on the biochemical, nutritional and functional aspects. Food Chemistry, 132, 1121–1132.
• [27] Garcia, D., Ramos, A.J., Sanchis, V., Marín, S. (2012). Effect of equisetum arvense and Stevia rebaudiana extracts on growth and mycotoxin production by aspergillus flavus and fusarium verticillioides in maize seeds as affected by water activity. International Journal of Food Microbiology, 153, 21–27.
• [28] Jayaraman, S., Saranana Manoharan, M., Illanchezian, S. (2008). In-vitro antimicrobial and antitumor activities of Stevia rebaudiana (asteraceae) leaf extracts. Trop. J. Pharm. Res., 7, 1143-1149.
• [29] Karaca, S. (2010). Stevia rebaudiana Yapraklarından Ekstrakte Edilen “Stevioside” İle “Rebaudioside A”nın Meyveli ve Gazlı İçeceklerde Kullanımı. Sakarya Üniversitesi, Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı, Yüksek Lisans Tezi, Sakarya.
• [30] Uçar-Sözmen, E. (2015). Şeker Otu (Stevia rebaudiana bertoni) Bitkisinin Bazı Verim ve Kalite Özellikleri Üzerine Farklı Azot Dozlarının Etkisi. Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Tarla Bitkileri Anabilim Dalı, Doktora Tezi, Antalya.
• [31] Yavaş-Sarıoğlu, A., 2015. Düşük Kalorili Dondurma Üretiminde Doğal Tatlandırıcı Olarak Stevya Ekstraktı Kullanımının Ürünün Kalite Kriterleri Üzerine Etkisi. Ege Üniversitesi, Fen Bilimleri Enstitüsü, Süt Teknolojisi Anabilim Dalı, Doktora Tezi. İzmir.
• [32] Savita, S.M., Sheela, K., Sunanda, S., Shankar, A.G., Ramakrishna, P. (2004). Stevia rebaudiana – A functional component for food industry. J. Hum. Eco., 15(4), 261- 264.
• [33] Carneıro, J.W.P., Munız, A.S. and Guedes, T.A. (1997). Greenhouse bedding plant production of Stevia rebaudiana (Bert) bertoni. Canadian Journal of Plant Science, 77, 473–474.
• [34] Chaturvedula, V.S.P., Upreti, M. and Prakash, I. (2011). Diterpene glycosides from Stevia rebaudiana. Molecules, 16, 3552-3562.
• [35] Gürleyik, E. (2010). Stevianın Rat Karaciğerinde Oksidan/Antioksidan Sistem Üzerine Etkisinin Araştırılması. Ankara Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Tıpta Uzmanlık Tezi, Ankara.
• [36] Mohammad, M.R., Mohammad, U.D., Sher, M.M., Habib, A.N., Lqbal, A.Q. (2007). In vitro clonal propagation and biochemical analysis of field established Stevia rebaudiana bertoni. Pakistan Journal of Botany, 39, 2467–2474.
• [37] Abou-Arab, A.E., Abou-Arab, A.A., Abou-Salem, F.M. (2010). Physico-chemical assessment of natural sweeteners steviosides produced from Stevia rebaudiana bertoni. Plant. Afric. J. Food Sci., 4, 269–280.
• [38] Marcinek, K., Krejpcio, Z. (2015). Stevia rebaudiana bertoni; chemical composition and functional properties. Acta Sci. Pol. Technol. Aliment., 14(2), 145–152.
• [39] Goyal, S., Samsher, R. (2010). Stevia a bio-sweetener: A review. International Journal of Food Sciences and Nutrition, 61, 1–10.
• [40] T.C. Şeker Kurumu. (2015). Yüksek Yoğunluklu Tatlandırıcılar (Mart).
• [41] Özçatalbaş, O. (2011). Sıfır Kalorili Şeker. http://www.antalyabugun.com/?page=makale&MID=13123.
• [42] ISO. (2012). Alternative sweeteners in a high sugar price environment. MECAS, (12), 04.
• [43] Yücesan, B. (2015). Stevia (Stevia rebaudiana) ve İkinci Şeker Devrimi. http://www.ulusaltarim.com/2824/Stevia-(Stevia-rebaudiana)-ve-ikinci-seker-devrimi. 15.02.2017.
• [44] The European Commission, Commission Regulation (EU) No. 1131/2011 of 11 November 2011 amending Annex II to Regulation (EC) No. 1333/2008 of the European Parliament and of the Council with regard to steviol glycosides, Official Journal of the European Union, L: 295-205.
• [45] Morlock, G.E., Meyer, S., Zimmermann, B.F., Roussel, J.M. (2014). High-performance thin-layer chromatography analysis of steviolglycosides in stevia formulations and sugar-free food products, and benchmarking with (ultra) high-performance liquid chromatography. Journal of Chromatography A, 1350, 102–111.
• [46] Smith, J., Vanstadin H. (1992). Subcellular pathway for glycoside synthesis. South African Journal of Science, 88, 206.
• [47] Chatsudthipong, V., Muanprasat, C. (2009). Stevioside and related compounds: therapeutic benefits beyond sweetness. Pharmacology & Therapeutics, 121, 41-54.
• [48] Geuns, J.M.C. (2003). Stevioside. Phytochemistry, 64, 913-921.
• [49] Sezgin, A.C., Koç F. (2016). Gastronomi alanında doğal tatlandırıcı stevia’nın kullanımı. Int. J. Acad. Res. Bus. Soc. Sci., 4(26), 255-265.
• [50] Kroyer, G. (2010). Stevioside and stevia-sweetener in food: application, stability and interaction with food ingriedients. J. Verbr. Lebensm., 5, 225-229.
• [51] Crammer, B., Ikan, R. (1986). Sweet glycosides from the stevia plant. Chem. Brit., 22, 915-916.
• [52] González, C., Tapia, M.S., Pérez, E., Dornier, M., Morel, G. (2014). Characterization of cultivars of Stevia rebaudiana bertoni produced in different countries. Bioagro, 26(2), 79-88.
• [53] Karakuş, M.Ş. (2013). Prebiyotik Lif İçeren Stevia Özü İlavesinin Çilek Aromalı Acidophilus-Bifidus Yoğurtlarının Bazı Kalite Özellikleri Üzerine Etkileri. Harran Üniversitesi, Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı, Yüksek Lisans Tezi, Şanlıurfa.
• [54] Zabihollahi, N. (2014). Düşük Kalorili Kek Üretiminde Kavrulmuş Buğday Unu, Stevya ve Polidekstroz Kullanım İmkanının Araştırılması. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı, Yüksek Lisans Tezi, Erzurum.
• [55] Nawar, W.W. (1985). Lipids. Food Chem., Edited by Fennema O.R., Marcel Dekker Inc., New York, 139-244 pp.
• [56] Turhan, S., Üstün Doğal, N.Ş. (2006). Antioksidanlar ve gıdalarda kullanımları. Türkiye 9. Gıda Kongresi; 24-26 Mayıs, Bolu, 273p.
• [57] Gökpınar, Ş., Koray, T., Akçiçek, E., Göksan, T., Durmaz, Y. (2006). Algal antioksidanlar. Ege Üniversitesi Su Ürünleri Dergisi, 23 Ek(1/1), 85-89-87.
• [58] Ruiz Ruiz, C., Moguel Ordoñez, Y.B., Matus Basto, Á., Segura Campos, M.R. (2015). Antioxidant capacity of leaf extracts from two Stevia rebaudiana bertoni varieties adapted to cultivation in Mexico. Nutr. Hosp., 31(3), 1163-1170.
• [59] Nizamlıoğlu, N.M., Nas, S. (2010). Meyve ve sebzelerde bulunan fenolik bileşikler; yapıları ve önemleri. Gıda Teknolojileri Elektronik Dergisi, 5(1), 20-35.
• [60] Nichenametla, S.N., Taruscio, T.G., Barney, D.L., Exon, J.H. (2006). A review of the effects and mechanisms of polyphenolics in cancer. Critical Reviews in Food Science and Nutrition, 46(2), 161-183.
• [61] Güleşci, N., Aygül, İ. (2016). Beslenmede yer alan antioksidan ve fenolik madde içerikli çerezler. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, 5(1), 109-129.
• [62] Criado, M.N., Civera, M., Martínez, A., Rodrigo, D. (2015). Use of Weibull distribution to quantify the antioxidant effect of Stevia rebaudiana on oxidative enzymes. LWT-Food Science and Technology, 60(1), 985-989.
• [63] Ghanta, S., Banerjee, A., Poddar, A., Chattopadhyay, S. (2007). Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (bert.) bertoni, a natural sweetener. Journal of Agricultural and Food Chemistry, 55(26), 10962–10967.
• [64] Wolwer-Rieck, U. (2012). The leaves of Stevia rebaudiana (bertoni), their constituents and the analyses there of: A review. Journal of Agricultural and Food Chemistry, 60(4), 886–895.
• [65] Cacciola, F., Delmonte, P., Jaworska, K., Dugo, P. (2011). Employing ultra high pressure liquid chromato graphy as the second dimension in a comprehensive two dimensional system for analysis of Stevia rebaudiana extracts. Journal of Chromatography A, 1218(15), 2012–2018.
• [66] Xi, Y., Yamaguchi, T., Sato, M., Takeuchi, M. (1998). Antioxidant Activity of Stevia rebaudiana. Nippon Shokuhin Kagaku Kaishi, 45(5), 310–316.
• [67] Tadhani, M., Subhash, R. (2006). In vitro antimicrobial activity of Stevia rebaudiana bertoni leaves. Trop J Pharm Res, 5(1), 557–560.
• [68] Shukla, S., Mehta, A., Menta, P., Bajpai, V. (2012). Antioxidant ability and phenolic content of aqueous leaf extract of Stevia rebaudiana bert. Experimental and Toxicologic Pathology, 64(7-8), 807–811.
• [69] Thomas, J., Glade, M. (2010). Stevia: It’s not just about calories. The Open Obesity Journal, 2, 101–109.
• [70] Bender, C., Graziano, S and Zimmermann, B.F. (2015). Study of Stevia rebaudiana bertoni antioxidant activities and cellular properties. International Journal of Food Sciences and Nutrition, 66(5), 553–558.
• [71] Gyawali, R., Ibrahim, S.A. (2014). Natural products as antimicrobial agents. Food Control, 46, 412-429.
• [72] Yücel Şengün İ., Yücel E. (2015). Antimicrobial properties of wild fruits. BioDiCon, 8(1), 69-77.
• [73] Cowan, M.M. (1999). Plant products as antimicrobial agents. CMR, 12(4), 564-582.
• [74] Cushnie, T.P.T., Lamb, A.J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5), 343–356.
• [75] Tomita, T., Sato, N., Arai, T., Shiraishi, H., Sato, M., Takeuchi, M. (1997). Bactericidal activity of a fermented hot-water extracts from Stevia rebaudiana bertoni and other food-borne pathogenic bacteria. Microbiology and Immunology, 41(12), 1005–1009.