Effects of Citrobacter freundii ATP Production and Oxygen Intake Two Different Plasmids Carrying the Vitreoscilla Hemoglobin Gene (vgb)

Yıl 2017, Cilt: 17 Sayı: 1, 28 - 33, 24.04.2017

Hüseyin Kahraman , Aslı Giray Kurt

Öz

In this study, the use of recombinant oxygen uptake system on production of ATP

production, oxygen uptake and cell mass was investigated. Citrobacter freundii and

their vgb recombinants were used. Citrobacter freundii, a broad host range plasmids

Cf[pMK 79] and Cf[pUC 8:15] was used. Best ATP production by Cf[pMK 79] (0.17 mg/

g). C f[pMK 7 9] s train t he l evel o f o xygene u ptake w as h igher t han C f[pUC 8 :15].

Whereas Cf[pMK 79] the OD600 values have been falling at the end of 48 hours.

Anahtar Kelimeler

Citrobacter freundii, Vitreoscilla hemoglobin gene (vgb), ATP production

Kaynakça

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