Potasyum Nitratın Dieng Platosu'ndan İzole Edilen Euglena Türlerinin Büyüme Hızı, Büyüme Kinetiği, Metabolitleri ve Lipid Profili Üzerindeki Etkisi

Öz

Euglena sp. yüksek düzeyde CO2 absorbe edebilir ve KNO3 ile geliştirilebilecek değerli bileşikler içerir. Bu çalışmanın amacı, KNO3'ün büyüme oranı üzerindeki etkisini belirlemek ve %15 CO2 ilavesinde popülasyon büyümesi, biyokütle, karbonhidrat, protein, pigment, α-tokoferol içeriği, lipid ve lipid profili için uygun doğrusal olmayan bir model belirlemektir. Uygulamalar, 0.25, 0.5 ve 0.75 g/L KNO₃ konsantrasyonlarının yanı sıra pozitif ve negatif kontrolleri (0.0 g/L) içermektedir. Bu çalışmanın sonuçlarına göre, 0.5 g/L KNO3 ilavesi en önemli büyüme oranını ve biyokütle, karbonhidrat, protein, klorofil-b, karoten ve lipid, 8.4×106 hücre/mL (hücre yoğunluğu) ve 0.6 ± 0.01 g/L ve sırasıyla 4.34 ± 0.055, 10.68 ± 0.38×10-3 g/L, 2.19 ± 0.13 µg/mL, 2.5 ± 0.00 g/L ve bu araştırma için en uygun model, R2 değeri >0.9 olan Gompertz modelidir. En yüksek klorofil-a 3,75 ± 0,00 µg/mL değeriyle kontrolde (-), en yüksek α-tokoferol içeriği ise 95 ± 0,00 µg/mL değeriyle 0,75 g/L KNO3 ilavesindedir. KNO3, 0.5 g/L ilavesine ek olarak 16 çeşit yağ asidi ile en geniş yağ asidi çeşitliliğini üretmiştir.

Anahtar Kelimeler

• Dieng suşu
• Gompertz modeli
• büyüme kinetiği modellemesi
• mikroalgal metabolit

Effect of Potassium Nitrate on Growth Rate, Growth Kinetics, Metabolites, and Lipid Profile of Euglena sp. Isolated from the Dieng Plateau

Öz

Euglena sp. can absorb high levels of CO2 and contains valuable compounds whose production can be enhanced by KNO₃ supplementation. This study aimed to determine the effect of KNO3 on the growth rate and to identify the most suitable non-linear model for population growth, biomass, carbohydrate, protein, pigment, α-tocopherol content, lipid concentration, and lipid profile of Euglena sp. cultured with 15% CO₂. Treatments consisted of KNO₃ concentrations of 0.25, 0.5, and 0.75 g/L as well as positive and negative controls (0.0 g/L). The results showed that the addition of 0.5 g/L KNO₃ produced the highest growth rate and the greatest biomass, carbohydrate, protein, chlorophyll-b, carotene, and lipid contents with respective values of 8.4 × 10⁶ cells/mL (cell density), 0.6 ± 0.01 g/L, 4.34 ± 0.055 g/L, 10.68 ± 0.38 × 10⁻³ g/L, 2.19 ± 0.13 µg/mL, and 0.25 ± 0.07 g/L. The most suitable growth model was the Gompertz model, which showed an R² value greater than 0.9. The highest chlorophyll-a content was observed in the negative control (3.75 ± 0.00 µg/mL), while the highest α-tocopherol content occurred with 0.75 g/L KNO₃ (95 ± 0.00 µg/mL). The 0.5 g/L KNO₃ treatment also produced the broadest range of fatty acids, with 16 types identified.

Anahtar Kelimeler

• Dieng strain
• Gompertz Model
• Growth Kinetics Modelling
• Microalgal Metabolite

Kaynakça

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