Antibacterial Effects of Microencapsulated Probiotic and Synbiotics

Öz

The present study aims to evaluate the inhibition effect of microencapsulated probiotic culture with prebiotics against two different pathogen enterococci strain. Lactobacillus rhamnosus was used as probiotic, inulin and fructooligosaccharide (FOS) were used as prebiotics. Microencapsulation maintained with three different combinations as only probiotic culture, probiotic culture with inulin and probiotic culture with FOS. In addition, the effect of inulin and FOS on survival of L. rhamnosus was evaluated. L. rhamnosus was microencapsulated by the extrusion technique and its antibacterial effect on clinical vancomycin-susceptible Enterococcus faecalis (VSEF) and clinical vancomycin-resistant Enterococcus faecium (VREF) growth was determined. According to the obtained results, microencapsulated probiotic culture with prebiotic addition showed inhibition effect on VREF growth. Furthermore, it was found that the survival rate of the probiotic culture cells varied according to the prebiotic. It was determined that inulin was more efficient on L. rhamnosus cell viability than FOS.

Anahtar Kelimeler

• Enterococcus,Lactobacillus rhamnosus,probiotic,synbiotic

Mikroenkapsüle Probiyotik ve Sinbiyotiklerin Antibakteriyel Etkisi

Öz

Bu çalışmada, prebiyotiklerle birlikte mikroenkapsüle edilen probiyotik kültürün iki farklı patojen enterokok gelişimi üzerine inhibe edici etkisi araştırılmıştır. Probiyotik olarak Lactobacillus rhamnosus (L. rhamnosus), prebiyotik olarak inulin ve fruktooligosakkarit (FOS) kullanılmıştır. Mikroenkapsülasyon, sadece probiyotik kültür, probiyotik kültür ile inülin, probiyotik kültür ile FOS olmak üzere 3 farklı kombinasyonda gerçekleştirilmiştir.  Ayrıca, FOS ve inülinin L. rhamnosus canlılığı üzerine etkisi belirlenmiştir. L. rhamnosus ekstrüzyon tekniği ile mikroenkapsüle edilmiş ve vankomisin-duyarlı Enterococcus faecalis (VSEF) ile vankomisin-dirençli Enterococcus faecium (VREF) üzerine antibakteriyel etkisi araştırılmıştır. Elde edilen sonuçlara göre, prebiyotik ilavesi ile mikroenkapsüle edilen probiyotik kültür VREF üzerine inhibe edici etki göstermiştir. Probiyotik hücrelerin canlılığının prebiyotik çeşidinden etkilendiği gözlenmiştir. L. rhamnosus canlılığı üzerinde inülinin FOS’a göre daha etkili olduğu tespit edilmiştir.

Anahtar Kelimeler

• Enterococcus,Lactobacillus rhamnosus,probiyotik,sinbiyotik

Kaynakça

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