Combine Effect of Vitamin C and Cadmium on the Aminoacid Content of Citrobacter Freundii

Öz

Bu çalışmada, Citrobacter freundii farklı konsantrasyonlarda kadmiyum içeren LB besi yerinde üretildi. Daha sonra kadmiyum içeren besi yerine değişik konsantrasyonlarda C vitamini katılarak, her iki durumda da üretilen bakterilerdeki aminoasit miktarları HPLC ile belirlendi. Kontrol ile 75, 100 ve 125 ppm Cd içeren gruplar karşılaştırıldığında alanin, arginin, asparajin, aspartik asit, sistein, glisin, histidin, isoleusin, leusin, lisin, fenilalanin, prolin, treonin, tirozin ve valin aminoasitlerinin miktarlarında artış (p<0.05), glutamik asit, glutamin, metiyonin, serin ve triptofan miktarlarında ise azalma p<0.05) gözlendi. Besi ortamına ilave edilen C vitamini sonucunda, kadmiyumun etkisine zıt etki göstererek miktarları artan aminoasitlerde azalma, miktarları azalan aminoasitlerde ise artış gözlenmiştir. Bu bulgulardan, kadmiyumun C. freundii üzerindeki olumsuz etkisini, besi yerine katılan C vitaminin azalttığı söylenebilir.

Anahtar Kelimeler

• C. Freundii
• Cadmium
• Vitamin C
• Aminoacid

Proje Numarası

Yok

Kaynakça

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