Antioxidant and anticholinesterase activities of Heliotropium dolosum, H. lasiocarpum and H. hirsutissimum growing in Turkey

Yıl 2019, , 1381 - 1391, 31.12.2019

Songül Karakaya , Mehmet Koca Ceyda Sibel Kılıç

https://doi.org/10.18185/erzifbed.530721

Cited By: 1

Öz

Alzheimer's
disease (AD) has encouraged science to find plants or biologic compounds which
can be utilized for prophylaxis of AD. Heliotropium
genus is presented by 16 taxa in Turkey and they contain pyrrolizidine
alkaloids, and certain are known to have antioxidant and anticholinesterase
activities. The paper displays the anticholinesterase and antioxidant
capacities of the aerial parts lyophilized aqueous extracts of H.
dolosum, H. lasiocarpum and H. hirsutissimum. Phenolic
contents and antioxidant activity of examples were determined by
Folin-Ciocalteu's, qualitative/quantitative DPPH and TBA. Anticholinesterase
activity was assessed by Ellman’s method. The total phenolic content of H. lasiocarpum extract was found the
highest (738.39 mg/g), however the total phenolic content of H. dolosum extract was found the lowest
(122.731 mg/g). Every example indicated that antioxidant activity with DPPH
test and H. lasiocarpum presented the
highest activities (69.99 μg/mL). Furthermore, the H. lasiocarpum extract indicated considerable inhibition towards
acetylcholinesterase (5.90%) and butyrylcholinesyterase (24.04%) enzymes. These
findings suggest that these Heliotropium species can be effective for
pharmaceutic products that achieve anticholinesterase and antioxidant
activities.

Anahtar Kelimeler

Antioxidant, Anticholinesterase

Türkiye'de yetişen Heliotropium dolosum, H. lasiocarpum ve H. hirsutissimum’un Antioksidan ve Antikolinestaraz Aktiviteleri

Öz

Alzheimer hastalığı (AD), bilimi AD'nin profilaksisinde kullanılabilecek bitkileri veya biyolojik bileşikleri bulmaya teşvik etmektedir. Heliotropium cinsi Türkiye'de 16 taksonla temsil edilir ve bu türler pirolizidin alkaloitleri içermektedir ve bazılarının antioksidan ve antikolinesteraz aktivitelere sahip olduğu bilinmektedir. Bu çalışmada, H. dolosum, H. lasiocarpum ve H. hirsutissimum'un toprak üstü kısımlarının liyofilize sulu ekstrelerinin antikolinesteraz ve antioksidan kapasiteleri incelenmiştir. Örneklerin fenolik içerikleri ve antioksidan aktivitesi Folin-Ciocalteu, kalitatif / kantitatif DPPH ve TBA ile belirlenmiştir. Antikolinesteraz aktivitesi Ellman metodu ile değerlendirilmiştir. En yüksek toplam fenolik içerik H. lasiocarpum ekstresinde (738.39 mg/g), ancak en düşük toplam fenolik içerik H. dolosum ekstresinde (122.731 mg/g) tespit edilmiştir. Her örnek DPPH testi ile antioksidan aktivite göstermiş ve en yüksek aktivite H. lasiocarpum'da (69.99 μg/mL) görülmüştür. Ayrıca, H. lasiocarpum ekstresi, sırasıyla asetilkolinesteraz (%5.90) ve butirilkolinesteraz (%24.04) enzimlerine karşı önemli ölçüde inhibisyon göstermiştir. Bu sonuçlar, bu Heliotropium türlerinin, antioksidan ve antikolinesteraz aktivitesine sahip farmasötik ürünler için etkili olabileceğini göstermektedir.

Anahtar Kelimeler

Antioksidan, Antikolinesteraz

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