Antioxidant and anticholinesterase activities of Heliotropium dolosum, H. lasiocarpum and H. hirsutissimum growing in Turkey

Yıl 2019, Cilt: 12 Sayı: 3, 1381 - 1391, 31.12.2019

Songül Karakaya , Mehmet Koca Ceyda Sibel Kılıç

https://doi.org/10.18185/erzifbed.530721

Cited By: 1

Öz

Alzheimer's
disease (AD) has encouraged science to find plants or biologic compounds which
can be utilized for prophylaxis of AD. Heliotropium
genus is presented by 16 taxa in Turkey and they contain pyrrolizidine
alkaloids, and certain are known to have antioxidant and anticholinesterase
activities. The paper displays the anticholinesterase and antioxidant
capacities of the aerial parts lyophilized aqueous extracts of H.
dolosum, H. lasiocarpum and H. hirsutissimum. Phenolic
contents and antioxidant activity of examples were determined by
Folin-Ciocalteu's, qualitative/quantitative DPPH and TBA. Anticholinesterase
activity was assessed by Ellman’s method. The total phenolic content of H. lasiocarpum extract was found the
highest (738.39 mg/g), however the total phenolic content of H. dolosum extract was found the lowest
(122.731 mg/g). Every example indicated that antioxidant activity with DPPH
test and H. lasiocarpum presented the
highest activities (69.99 μg/mL). Furthermore, the H. lasiocarpum extract indicated considerable inhibition towards
acetylcholinesterase (5.90%) and butyrylcholinesyterase (24.04%) enzymes. These
findings suggest that these Heliotropium species can be effective for
pharmaceutic products that achieve anticholinesterase and antioxidant
activities.

Anahtar Kelimeler

Antioxidant, Anticholinesterase

Türkiye'de yetişen Heliotropium dolosum, H. lasiocarpum ve H. hirsutissimum’un Antioksidan ve Antikolinestaraz Aktiviteleri

Öz

Alzheimer hastalığı (AD), bilimi AD'nin profilaksisinde kullanılabilecek bitkileri veya biyolojik bileşikleri bulmaya teşvik etmektedir. Heliotropium cinsi Türkiye'de 16 taksonla temsil edilir ve bu türler pirolizidin alkaloitleri içermektedir ve bazılarının antioksidan ve antikolinesteraz aktivitelere sahip olduğu bilinmektedir. Bu çalışmada, H. dolosum, H. lasiocarpum ve H. hirsutissimum'un toprak üstü kısımlarının liyofilize sulu ekstrelerinin antikolinesteraz ve antioksidan kapasiteleri incelenmiştir. Örneklerin fenolik içerikleri ve antioksidan aktivitesi Folin-Ciocalteu, kalitatif / kantitatif DPPH ve TBA ile belirlenmiştir. Antikolinesteraz aktivitesi Ellman metodu ile değerlendirilmiştir. En yüksek toplam fenolik içerik H. lasiocarpum ekstresinde (738.39 mg/g), ancak en düşük toplam fenolik içerik H. dolosum ekstresinde (122.731 mg/g) tespit edilmiştir. Her örnek DPPH testi ile antioksidan aktivite göstermiş ve en yüksek aktivite H. lasiocarpum'da (69.99 μg/mL) görülmüştür. Ayrıca, H. lasiocarpum ekstresi, sırasıyla asetilkolinesteraz (%5.90) ve butirilkolinesteraz (%24.04) enzimlerine karşı önemli ölçüde inhibisyon göstermiştir. Bu sonuçlar, bu Heliotropium türlerinin, antioksidan ve antikolinesteraz aktivitesine sahip farmasötik ürünler için etkili olabileceğini göstermektedir.

Anahtar Kelimeler

Antioksidan, Antikolinesteraz

Kaynakça

• Alzheimer’s Association. 2018. “Alzheimer’s disease facts and figures”, Alzheimer's & Dementia, 14, 367-429.
• Aboelmagd, M., Elokely, K.,. Zaki, M.A., Said, A., Haggag, E.G., Ros, S.A. 2018. “Anti-inflammatory of pyrrolizidine alkaloids from Heliotropium digynum”, Medicinal Chemistry Research, 27, 1066-1073.
• Arıhan, O., Karakaya, S., Kılıc, C.S., Erkec, O.E., Kara, M., Duman, H. 2018. “Effect of Heliotropium hirsutissimum, Heliotropium dolosum and Helıotropıum Lasıocarpum Methanolıc Extracts in Pentylentetrazol Induced Convulsions on Mice”, J. Fac. Pharm. Ankara, 42(3), 13-19.
• Boye, A. 2010” Further Studies on the Pharmacological and Toxicological Profile of Crude Extracts Of Heliotropium indicum Linn.”, A Master THESIS, The Department Of Pharmacology, Kwame Nkrumah University of Science & Technology Faculty of Pharmacy and Pharmaceutical Sciences, Kwame, 47.
• Çeribaşı S. (2010). “Etlik Piliçlerde Diyetsel Heliotropium circinatum Toksikasyonu Üzerine Patolojik ve Biyokimyasal İncelemeler”, Doktora Tezi, Songül Çeribaşi Elazığ, Doktora Tezi-Sayfa 6.
• Davinelli, S., Sapere, N., Zella, D., Bracale, R., Intrieri, M., Scapagnini, G. 2012. “Pleiotropic protective effects of phytochemicals in Alzheimer's disease”, Oxidative Med. Cell., Longev 2012, 386-397. Davis, P. H. (1972). “Flora of Turkey and the East Aegean Islands 2nd ed.”, Edinburgh University Press, Edinburgh, 4, 469-471.
• Ellman, G.L., Courtney, K.D., Andresjr, V., Featherstone, R.M. 1961. “A new and rapid colorimetric determination of acetylcholinesterase activity”, Biochem Pharmacol., 7, 88-95.
• Ferreira, A., Proenc, C., Serralheiro, M.L.M., Araujo, M.E.M. 2006. “The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal”, J Ethnopharmacol, 108, 3-37.
• Granato, D., Shahidi, F., Wrolstad, R., Kilmartin, P., Melton, L.D., Hidalgo, F.J., Miyashita, K., Camp, J., Alasalvar, C., Ismail, A.B., Elmore, S., Birch, G.G., Charalampopoulos, D., Astley, S.B., Pegg, R., Zhou, P., Finglas, P. 2018. “Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods?”, Food Chem., 264, 471-475.
• Güner. A. (2012) “Türkiye Bitkileri Listesi (Damarlı Bitkiler)”, Nezahat Gökyiğit Botanic Garden Publications, İstanbul, 227-229.
• Karakaya, S., Koca, M., Kilic, C.S., Coskun, M. 2018. “Antioxidant and anticholinesterase activities of Ferulago syriaca Boiss. and F. isaurica Peșmen growing in Turkey”, Med Chem Res., 27, 1843-1850.
• Karch, S., Broichhagen, J., Schneider, J., Böning, D., Hartmann, S., Schmid, B., Tripal, P., Palmisano, R., Alzheimer, C., Johnsson, K., Huth, T. 2018. „A new fluorogenic small-molecule labeling tool for surface diffusion analysis and advanced fluorescence imaging of β‑site amyloid precursor protein-cleaving enzyme 1 based on silicone rhodamine: SiR-BACE1”, J. Med. Chem, 61, 6121-6139.
• Khan, H., Khan, M.A., Gul, F., Hussain, S., Ashraf, N. 2013. “In-vivo antipyretic activity of extracts of Heliotropium strigosum”, Medicinal Chemistry & Drug Discovery, 4(1), 55-61.
• Koffuor, G.A., Boye, A., Ameyaw, E.O., Amoateng, P., Abaitey, A.K. 2012. “Hypotensive effect of an aqueous extract of Heliotropium indicum Linn (Boraginaceae)”, International Research Journal of Pharmacy and Pharmacology, 2(5), 103-109.
• Luz, D.A., Pinheiro, A.M., Silva, M.L., Monteiroa, M.C., Prediger, R.D., Maiaa, C.S.F., Andrad, E., Júniora, F. 2016. “Ethnobotany, phytochemistry and neuropharmacological effects of Petiveria alliacea L. (Phytolaccaceae): A review”, J Ethnopharmacol, 185, 182-201.
• Perry, E.K., Pickering, A.T., Wang, W.W., Houghton, P., Perry, N.L. 1998. “Medicinal Plants and Alzheimer's Disease: Integrating Ethnobotanical and Contemporary Scientific Evidence”, The Journal of Alternative and Complementary Medicine, 4, 419-428.
• Rossi, F., Jullian, V., Pawlo, W.R., Roine´, S., Haddad, M., Darius, H., Mazouni, N., Chinain, M., Laurent, D. 2012. “Protective effect of Heliotropium foertherianum (Boraginaceae) folk remedy and its active compound, rosmarinic acid,against a Pacific ciguatoxin”, Journal of Ethnopharmacology, 143(2012), 33-40.
• Sadaoui, N., Bec, N., Barragan-Montero, V., Kadrie, N., Cuisinier, F., Larroque, C, Karim, A., Bachra, K. 2018. “The essential oil of Algerian Ammodaucus leucotrichus Coss. & Dur. and its effect on the cholinesterase and monoamine oxidase activities”, Fitoterapia 130, 1-5.
• Senol, F.S., Woz´niak, K.S.K., Khan, M.T.H., Orhan, I.E., Sener, B., Głowniak, K. 2011. “An in vitro and in silico approach to cholinesterase inhibitory and antioxidant effects of the methanol extract, furanocoumarin fraction, and major coumarins of Angelica officinalis L. fruits”, Phytochem Lett, 4, 462-467.
• Shah, S.M., Hussain, S., Khan, A.U., Shah, A.H., Khan, H., Barkatullah, U.F. 2012. “Cytotoxic and phytotoxic actions of Heliotropium strigosum”, Toxicology and Industrial Health, 31, 1-4.
• Singh, B., Dubey, M.M. 2001. “Estimation of Triterpenoids from Heliotropium marifolium Koen. ex Retz". In vivo and In vitro. I. Antimicrobial Screening”, Phytother. Res., 15, 231-234.
• Sytar, O., Bruckova, K., Hunkova, E., Zivcak, M., Kiessoun, K., Brestic, M. 2015. “The application of Muliplex flourimetric sensor for analysis flavonoids content in the medical herbs family Asteraceae, Lamiaceae, Rosaceae”, Biological research, 48(5), DOI: 10.1186/0717-6287-48-5.
• Ustun, O., Senol, F.S., Kurkcuoglu, M., Orhan, I.E., Kartal, M., Baser, K.H.C. 2012. “Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol”, Industrial Crops and Products, 38, 115-123.
• World Health Organization. 2015. “The epidemiology and impact of dementia. WHO”, http://www.who.int/mental_health/neurology/dementia/dementia_thematicbrief_epidemiology.pdf. Accessed date:16.09.2019.
• Yerdelen, K.Ö., Tosun, E. 2015. „Synthesis, docking and biological evaluation of oxamide and fumaramide analogs as potential AChE and BuChE inhibitors”, Med Chem Res., 24, 588-602.