A RESEARCH ON THE VIRAL RESPIRATORY SYSTEM DISEASES COMPLEX IN THE CATTLE OF SAMSUN REGION IN TERMS OF CLINICAL, HAEMATOLOGICAL AND ACUTE PHASE PROTEINS

Öz

The purpose of this study was to research the presence of viral respiratory system diseases which are very common in Samsun and to analyze the relationship between these diseases and hematological, blood gases and acute phase proteins. For this purpose, 404 cattle older than nine months, which were not vaccinated with the Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea (BVD), Para influenza -3Virus (PI-3), and Bovine Respiratory Synctial Virus (BRSV) viruses, were used. Of the 404 cattle used in the study, 200 were included in the disease group and 204 cattle, which clinically did not show any diseases symptoms, were included in the healthy group. The antibody seroprevalance rates of the disease group cattle against IBR, BVD, BRSV, PI-3 viruses were found to be 44.5%, 55.5%, 93.5%, 87% respectively. 4 of the cattle in the disease group did not develop antibody against any of the aforementioned viruses. The antigen ELISA rates of the disease group cattle against IBR, BVD, BRSV, PI-3 viruses were found to be 0.5%, 0%, 1%, 1.5% respectively. The antibody seroprevalance rates of the healthy group cattle against IBR, BVD, BRSV, PI-3 viruses were found to be 48.5%, 64.2%, 91.6%, 90.6% respectively. 2 of the cattle in the healthy group did not develop antibody against any of the aforementioned viruses and none of the antigen ELISA test results of the healthy group cattle was positive. When the pH, pCO2, pO2 and O2SAT values in blood gas analyses were compared, statistically significant differences were not found between the two groups. There were statistically significant differences only in terms of actual bicarbonate (HCO3) (P<0.01), base excess (BE) (P<0.05) and total CO2 (P<0.01) values. Of the hematological parameters, only (P<0.01), WBC (P<0.01) and Monocyte (P<0.05) values of the disease group cattle were significantly higher than those of the healthy group cattle. When the red blood cell (RBC), hemoglobin (Hgb) and hematocrit (Hct) values and lymphocytes and granulocyte percentages of the two groups were compared, statistically significant differences were not found between the two groups. While acute phase proteins Haptoglobin (Hp) (P<0.01), Serum Amyloid A (SAA) (P<0.001), C Reactive Protein (CRP) (P<0.01) and Fibrinogen (Fb) (P<0.01) concentrations were found to be statistically higher in the disease group cattle, negative acute phase protein Albumin concentration (P<0.01) was found to be significantly lower. As a result, it was concluded that the serologic test results for the viral elements in cattle were similar to the results of the previous studies in our country, it was also concluded that viral respiratory system diseases showed changes on blood gases and hematological parameters in animals and especially increased acute phase protein levels significantly. 

Anahtar Kelimeler

• Acute phase protein
• blood gases
• bovine
• clinical
• hematological

SAMSUN YÖRESİNDEKİ SIĞIRLARDA VİRAL SOLUNUM SİSTEMİ HASTALIKLARI KOMPLEKSİNİN KLİNİK, HEMATOLOJİK VE AKUT FAZ PROTEİNLERİ YÖNÜNDEN ARAŞTIRILMASI

Öz

Bu çalışmada Samsun yöresinde sık görülen viral solunum sistemi hastalıklarının varlığı ve hematolojik, kan gazları ve akut faz proteinlerle ilişkisinin araştırılması amaçlandı. Bu amaçla çalışmada InfectiousBovineRhinotracheitis (IBR), BovineViralDiarrhea (BVD), Parainfluenza -3Virus (PI-3) ve BovineRespiratorySynctialVirus(BRSV) virüslerle aşılanmamış, yaşları dokuz aydan büyük olan 200 adeti hasta ve 204 adeti ise sağlıklı olan toplam 404 adet sığır kullanıldı. Hastalık grubundaki sığırların (n= 200) IBR, BVD, PI-3, BRSV virüsüne karşı oluşturulan antikor seroprevalansı oranları sırasıyla % 44.5, % 55.5, % 93.5, % 87 olarak tespit edildi. Hastalık grubundaki 4 sığırda söz konusu virüslerin hiçbirine karşı antikor oluşmadığı belirlendi. Hastalık grubundaki sığırların IBR, BVD, BRSV, PI-3 virüsüne karşı oluşan antijen ELISA oranları sırasıyla, % 0.5, % 0, % 1, % 1.5 olarak tespit edildi. Sağlıklı grubundaki sığırların (n= 204) IBR, BVD, BRSV, PI-3 virüsüne karşı oluşan antikor seroprevalansı oranları sırasıyla % 48.5, % 64.2, % 91.6, % 90.6 olarak belirlendi. Sağlıklı grubundaki 2 sığırda söz konusu virüslerin hiçbirine karşı antikor oluşmadığı ve sağlıklı grubundaki sığırların hiçbirinde antijen ELISA testinin pozitif sonuç vermediği tespit edildi. Kan gazları analizlerinde pH, pCO2, pO2 ve O2SAT değerleri karşılaştırıldığında her iki grup arasında istatistiksel olarak önemli farklılıklar tespit edilemedi. Sadece aktüel bikarbonat (HCO3) (P<0.01), baz fazlalığı değerleri (P<0.05) ve total CO2 (P<0.001) değerleri yönünden istatistiksel olarak anlamlı farklılıklar bulundu. Hematolojik parametrelerden sadece PLT (P<0.01), WBC (P<0.01) ve monosit (P<0.05) değerleri hasta hayvanlarda sağlıklı olanlara göre istatistiksel olarak önemli derecede yüksek bulundu. Her iki grubun eritrosit (RBC), hemoglobin (Hgb) ve hematokrit (Hct) değerleri ile lenfosit ve granülosit %’lerikarşılaştırıldığında gruplar arasında istatistiksel olarak önemli farklılıkların bulunmadığı tespit edildi. Hasta grubundaki hayvanlarda akut faz proteinlerinden Haptoglobin (Hp) (P<0.01), Serum Amiloid A (SAA) (P<0.01), C Reaktif Protein (CRP) (P<0.01) ve Fibrinojen (Fb) (P<0.01) konsantrasyonları sağlıklılara göre istatistiksel olarak yüksek bulunurken, negatif akut faz protein olarak değerlendirilen albümin konsantrasyonu (P<0.01) ise sağlıklılara göre önemli derecede düşük bulundu. Sonuç olarak sığırlarda viral etkenlere yönelik olarak elde edilen serolojik test sonuçlarının ülkemizde daha önce yapılmış olan çalışma sonuçlarına benzer olduğu, viral kökenli solunum sistemi hastalıklarının hayvanlarda kan gazları ve hematolojik parametreleri üzerinde değişimler gösterdiği ve özellikle akut faz protein düzeylerini önemli ölçüde arttırdığı tespit edildi.

Anahtar Kelimeler

• Akut Faz Proteinleri
• Hematolojik
• Kan Gazları
• Klinik
• Sığır

Kaynakça

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