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MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ

Yıl 2004, Cilt: 13 Sayı: 2, 15 - 21, 01.09.2004

Öz

Bu çalışmada yüzeyel mesane kanserli hastaların transüretral rezeksiyon (TUR) doku örneklerinden hazırlanan tranzisyonel karsinom hücre kültürlerinde mitomisin-C (MMC) ve propolisin mutagenik etkilerine bakıldı. Çalışmamızda mesane kanserli doku kültürlerine 0.1 ml propolis (8 g propolis + %8’lik 15 ml L-Lizin), 1.6 µl mitomisin-C (0.1 mg/ml) ve 0.1 ml L-Lizin (80 mg L-Lizin + 1 ml distile su) ilave edilerek, mikronükleus üzerine etkileri değerlendirildi. Sonuçlarımız propolis ve mitomisinC’nin kontrol grubuna hücrelerdeki mikronükleus (MN) oranını arttırdığını göstermektedir (p<0.05). Hem propolis hem de mitomisin-C mikronükleus oranını arttırıcı etkilerinden dolayı mutagen maddelerdir

Kaynakça

  • Ghisalberti EL. Propolis a review. Bee World 1979, 60:59-84.
  • Ghisalberti EL, Jefferies PR, Lanteri R, Matisons J. Constituents of propolis. Experientia 1978, 34:157-158.
  • Grange JM, Davey RW. Antibacterial properties of propolis (bee glue). J Royal Soc Med 1990, 83:159-160.
  • Greenaway W, May J, Scaysbrook T, Watley FR. Identification by gas chromatography-mas spectromery of 150 compounds in propolis. Z Naturforsch 1991, 46:111-121.
  • Krol W, Czuba Z, Scheller S, Paradowski Z, Shani F. Structure-activity relationship in the ability of flavonoids to inhibit chemiluminescence. Journal of Ethnopharmacology 1993, 41:121-126.
  • Kujumgiev A, Tsvetkova I, Serkedjieva YU, et al. Antibacterial, antifungal and antiviral activity of propolis different geographic origin. J Ethnopharmacol 1999, 64:235-240.
  • Basnet P, Matsushige K, Hase K, Kadota S. Potent antihepatotoxic activity of dicaffe oylquinic acids from propolis. Biol Pharm Bull 1996, 19:1479-1484.
  • Amoros M, Savuager F, Girre L, Cormier M. In vitro antiviral activity of propolis. Apidologie 1992, 23:1732-1740.
  • Mirzoeva OK, Calder PC. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostaglandins Leukotr Essent Fatty Acids 1996, 55:441-449.
  • Anafarta K, Göğüş O, Bedük Y, Arıkan N. Temel Üroloji. Güneş Kitabevi, Ankara 1998, pp 708-713.
  • Goldin A, Serpick AA, Mantel N. Experimental screening procedures and clinical predictability value. Cancer Chemotherapy Report 1966, 50:173-218.
  • Eroğlu HE. Mesane Kanserli Hastaların Doku Örneklerinde Propolis ve Mitomisin-C’nin Mikronükleus, Kardeş Kromatid Değişimi, Kromozom Analizi ve Mitotik İndeks Parametrelerine Etkisinin Karşılaştırılması. Yüksek Lisans Tezi, Erciyes Ün. Sağlık Bilimleri Enstitüsü, Kayseri 2004, ss 89.
  • Sarto F, Tomanin R, Giacomelli L, Canova A, Raimondi F. Evaluation of chromosomal aberrations in lymphocytes and micronuclei in lymphocytes, oral mucosa and hair root cells of patiens under antiblastic therapy. Mutation Research 1990, 228:157-169.
  • Krishnaja AP, Sharma NK. Ascorbic acid potentiates mitomycin C-induced micronuclei and sister chromatid exchanges in human peripheral blood lymphocytes in vitro. Teratog Carcinog Mutagen 2003, 1:99-112.
  • Grisolia CK. A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin-C and various pesticides. Mutatation Research 2002, 518:145-150.
  • Nikolov N, Marekov N, Bankova V, et al. Method for the preparation of water-soluble derivative of propolis. Veterinary and Comparative Oncology 1987, 1:216-220.
  • Matsuno T, Jung SK, MatsumotoY, Saito M, Morikawa J. Preferential Cytotoxicity to Tumor Cells of 3,5-Diprenyl-4- Hydroxycinnamic Acid (Artepillin C) Isolated from Propolis. Anticancer Research 1997, 17:3565-3568.
  • Prasad S, Naik P, Vijayalaxmi KK. Efficiency of Coleus aromaticus extract in modifying cyclophosphamide and mitomycin-C induced clastogenicity in mouse bone marrow cells. Indian J Exp Biol 2002, 40:1020-1025.
  • Möller M, Stopper H, Haring M, Schleger Y, Epe B, Adam W, Saha-Möller CR. Genotoxicity induced by furocoumarin hydroperoxides in mammalian cells upon UVA irradiation. Biochem Biophys Res Commun 1995, 216:693- 701.
  • Heddle AJ, Stuart E, Salomone MF. The Bone- marrow Micronucleus Test: Handbook of Mutagenicity Test Procedures. BJ Kilbey Elsevier Science Publ BV Second Edition 1984, 20:441-457.
  • Eroğlu HE, Tatlışen A, Özkul Y. Mesane Kanserinde Türk Propolisi ve Mitomisin-C’nin Mitotik Parametrelerinin Karşılaştırılması, VI. Ulusal Prenatal Tanı ve Tıbbi Genetik Kongresi Bildiri Kitabı, Antalya 21-24/Nisan 2004, ss 131.
  • Ortiz R, Medina H, Rodriguez L, Gonzalez- Marquez H, Cortes E. Spontaneous and mitomycin C-induced micronuclei in peripheral blood reticulocytes from severely malnourished rats. Environ Mol Mutagen 2004, 43:179-185.

Effects of Propolis and Mitomycın-C on Micronucleusin Tissue Cultures of Bladder Cancer

Yıl 2004, Cilt: 13 Sayı: 2, 15 - 21, 01.09.2004

Öz

In this study, we have tested the mutagenic effects of mitomycin-C (MMC) and propolis on transitional carcinoma cell cultures. Tissue samples were obtained from transurethral resection (TUR) tissue of patients with superficial bladder cancer. In this study, 0.1 ml propolis (8 g propolis + 8% 15 ml L-Lysin), 1.6 µl mitomycin-C (0.1 mg/ml) and 0.1 ml L-Lysin (80 mg L-Lysin + 1 ml distilled water) were added to cancer tissue cultures and the effects of these solutions on the micronucleus (MN) rate were evaluated. Propolis and mitomycin-C increased the micronucleus rate of the cell when compared to untreated control (p<0.05). Our results showed that both propolis and mitomycin-C are mutagenic materials, because of their micronucleus increasing effects

Kaynakça

  • Ghisalberti EL. Propolis a review. Bee World 1979, 60:59-84.
  • Ghisalberti EL, Jefferies PR, Lanteri R, Matisons J. Constituents of propolis. Experientia 1978, 34:157-158.
  • Grange JM, Davey RW. Antibacterial properties of propolis (bee glue). J Royal Soc Med 1990, 83:159-160.
  • Greenaway W, May J, Scaysbrook T, Watley FR. Identification by gas chromatography-mas spectromery of 150 compounds in propolis. Z Naturforsch 1991, 46:111-121.
  • Krol W, Czuba Z, Scheller S, Paradowski Z, Shani F. Structure-activity relationship in the ability of flavonoids to inhibit chemiluminescence. Journal of Ethnopharmacology 1993, 41:121-126.
  • Kujumgiev A, Tsvetkova I, Serkedjieva YU, et al. Antibacterial, antifungal and antiviral activity of propolis different geographic origin. J Ethnopharmacol 1999, 64:235-240.
  • Basnet P, Matsushige K, Hase K, Kadota S. Potent antihepatotoxic activity of dicaffe oylquinic acids from propolis. Biol Pharm Bull 1996, 19:1479-1484.
  • Amoros M, Savuager F, Girre L, Cormier M. In vitro antiviral activity of propolis. Apidologie 1992, 23:1732-1740.
  • Mirzoeva OK, Calder PC. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostaglandins Leukotr Essent Fatty Acids 1996, 55:441-449.
  • Anafarta K, Göğüş O, Bedük Y, Arıkan N. Temel Üroloji. Güneş Kitabevi, Ankara 1998, pp 708-713.
  • Goldin A, Serpick AA, Mantel N. Experimental screening procedures and clinical predictability value. Cancer Chemotherapy Report 1966, 50:173-218.
  • Eroğlu HE. Mesane Kanserli Hastaların Doku Örneklerinde Propolis ve Mitomisin-C’nin Mikronükleus, Kardeş Kromatid Değişimi, Kromozom Analizi ve Mitotik İndeks Parametrelerine Etkisinin Karşılaştırılması. Yüksek Lisans Tezi, Erciyes Ün. Sağlık Bilimleri Enstitüsü, Kayseri 2004, ss 89.
  • Sarto F, Tomanin R, Giacomelli L, Canova A, Raimondi F. Evaluation of chromosomal aberrations in lymphocytes and micronuclei in lymphocytes, oral mucosa and hair root cells of patiens under antiblastic therapy. Mutation Research 1990, 228:157-169.
  • Krishnaja AP, Sharma NK. Ascorbic acid potentiates mitomycin C-induced micronuclei and sister chromatid exchanges in human peripheral blood lymphocytes in vitro. Teratog Carcinog Mutagen 2003, 1:99-112.
  • Grisolia CK. A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin-C and various pesticides. Mutatation Research 2002, 518:145-150.
  • Nikolov N, Marekov N, Bankova V, et al. Method for the preparation of water-soluble derivative of propolis. Veterinary and Comparative Oncology 1987, 1:216-220.
  • Matsuno T, Jung SK, MatsumotoY, Saito M, Morikawa J. Preferential Cytotoxicity to Tumor Cells of 3,5-Diprenyl-4- Hydroxycinnamic Acid (Artepillin C) Isolated from Propolis. Anticancer Research 1997, 17:3565-3568.
  • Prasad S, Naik P, Vijayalaxmi KK. Efficiency of Coleus aromaticus extract in modifying cyclophosphamide and mitomycin-C induced clastogenicity in mouse bone marrow cells. Indian J Exp Biol 2002, 40:1020-1025.
  • Möller M, Stopper H, Haring M, Schleger Y, Epe B, Adam W, Saha-Möller CR. Genotoxicity induced by furocoumarin hydroperoxides in mammalian cells upon UVA irradiation. Biochem Biophys Res Commun 1995, 216:693- 701.
  • Heddle AJ, Stuart E, Salomone MF. The Bone- marrow Micronucleus Test: Handbook of Mutagenicity Test Procedures. BJ Kilbey Elsevier Science Publ BV Second Edition 1984, 20:441-457.
  • Eroğlu HE, Tatlışen A, Özkul Y. Mesane Kanserinde Türk Propolisi ve Mitomisin-C’nin Mitotik Parametrelerinin Karşılaştırılması, VI. Ulusal Prenatal Tanı ve Tıbbi Genetik Kongresi Bildiri Kitabı, Antalya 21-24/Nisan 2004, ss 131.
  • Ortiz R, Medina H, Rodriguez L, Gonzalez- Marquez H, Cortes E. Spontaneous and mitomycin C-induced micronuclei in peripheral blood reticulocytes from severely malnourished rats. Environ Mol Mutagen 2004, 43:179-185.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA85MU54JG
Bölüm Araştırma Makalesi
Yazarlar

Halil Erhan Eroğlu Bu kişi benim

Atilla Tatlışen Bu kişi benim

Yusuf Özkul Bu kişi benim

Yayımlanma Tarihi 1 Eylül 2004
Gönderilme Tarihi 1 Eylül 2004
Yayımlandığı Sayı Yıl 2004 Cilt: 13 Sayı: 2

Kaynak Göster

APA Eroğlu, H. E., Tatlışen, A., & Özkul, Y. (2004). MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ. Sağlık Bilimleri Dergisi, 13(2), 15-21.
AMA Eroğlu HE, Tatlışen A, Özkul Y. MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ. JHS. Eylül 2004;13(2):15-21.
Chicago Eroğlu, Halil Erhan, Atilla Tatlışen, ve Yusuf Özkul. “MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ”. Sağlık Bilimleri Dergisi 13, sy. 2 (Eylül 2004): 15-21.
EndNote Eroğlu HE, Tatlışen A, Özkul Y (01 Eylül 2004) MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ. Sağlık Bilimleri Dergisi 13 2 15–21.
IEEE H. E. Eroğlu, A. Tatlışen, ve Y. Özkul, “MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ”, JHS, c. 13, sy. 2, ss. 15–21, 2004.
ISNAD Eroğlu, Halil Erhan vd. “MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ”. Sağlık Bilimleri Dergisi 13/2 (Eylül 2004), 15-21.
JAMA Eroğlu HE, Tatlışen A, Özkul Y. MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ. JHS. 2004;13:15–21.
MLA Eroğlu, Halil Erhan vd. “MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ”. Sağlık Bilimleri Dergisi, c. 13, sy. 2, 2004, ss. 15-21.
Vancouver Eroğlu HE, Tatlışen A, Özkul Y. MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ. JHS. 2004;13(2):15-21.