EXTRACTION OF BIOACTIVE COMPOUNDS FROM FENUGREEK LEAVES BY MACERATION WITH D-OPTIMAL DESIGN

Öz

In this study, optimum extraction conditions of bioactive compounds from fenugreek leaves (Trigonella-foenum graecum L.) were investigated using response surface methodology and the extracts having the highest total phenolic content, total flavonoid compounds, antioxidant activity and total saponin content were achieved. The independent process variables were solvent mixture ratio (water and ethanol, 0-100%), temperature (25-65°C) and sample–solvent ratio (10-50 g/L), and a constant extraction time of 120 minutes was used for all the design points. The experimental study was arranged according to D-optimal combined design and the process conditions were optimized using desirability function approach. Results showed that the extraction of phenolic compounds and antioxidant activity were increased at increasing water ratios and temperature and decreasing sample-solvent ratio. Saponins were extracted better when 25% ethanol was used as solvent. The optimum extraction conditions were determined as 100% of water, 49.71°C of temperature, and 10 g/L of sample–solvent ratio.

Anahtar Kelimeler

• fenugreek leaves
• phenolic compounds
• extraction
• optimization
• saponin

D-OPTİMAL TASARIM İLE ÇEMEN OTU YAPRAKLARINDAN BİYOAKTİF BİLEŞENLERİN MASERASYON YOLUYLA EKSTRAKSİYONU

Öz

Bu çalışmada çemen otu (Trigonella-foenum graecum L.) yapraklarından biyoaktif bileşenlerin optimum ekstraksiyon koşulları yanıt yüzey yöntemi kullanılarak belirlenmiş ve en yüksek toplam fenolik madde, toplam flavonoid, antioksidan aktivite ve toplam saponin içeriğine sahip ekstraktlar elde edilmiştir. Çözgen karışım oranı (su ve etanol, %0-100), sıcaklık (25-65°C) ve örnek–çözgen oranı (10-50 g/L) bağımsız işlem değişkenleri olarak seçilmiş ve tasarımın tüm noktalarında 120 dakikalık ekstraksiyon süresi sabit olarak uygulanmıştır. Deneysel çalışma D-optimal birleşik tasarıma göre düzenlenmiş ve istenirlik fonksiyonu yaklaşımı kullanılarak işlem koşulları optimize edilmiştir. Sonuçlara göre çözgende bulunan suyun yüzdesi ve ekstraksiyon sıcaklığı arttıkça, örnek–çözgen oranı azaldıkça fenolik bileşenlerin miktarı artış göstermiştir. Saponinler ise çözgen olarak %25 etanol kullanıldığında daha iyi ekstrakte edilmişlerdir. Optimum ekstraksiyon koşulları %100 saf su, 49.71°C ekstraksiyon sıcaklığı ve 10 g/L örnek–çözgen oranı olarak belirlenmiştir.

Anahtar Kelimeler

• çemen otu yaprağı
• fenolik bileşenler
• ekstraksiyon
• optimizasyon
• saponin

Kaynakça

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