ZIZIPHUS JUJUBE (HÜNNAP) İÇ VE ÇEKİRDEK DOKULARINDAN FENOLİKLERİN IN VITRO SİNDİRİM SIRASINDA BAZI BİYOAKTİF ÖZELLİKLERİNDEKİ DEĞİŞİMİN ARAŞTIRILMASI

Öz

sindirim sırasında anjiyotensin-I dönüştürücü enzim (ADE) inhibisyon, α-amilaz inhibisyon ve antioksidan aktivitesi ile toplam fenolik madde (TFM) ve toplam monomerik antosiyanin miktarı (TMAM) üzerindeki değişiklikler incelenmiştir. In vitro sindirim sonrasında, çekirdek ve iç ekstraktlarındaki biyoerişilebilir fenolik fraksiyonlar sırasıyla %23.24±4.46 ve %9.43±0.24 olarak hesaplanmıştır. Ayrıca, çekirdek ekstraktlarında toplam monomerik antosiyaninlerin biyoerişilebilirliği (147.83±9.20%) iç ekstraktların toplam monomerik antosiyaninlerinin biyoerişilebilirliğinden (15.76±3.89%) daha yüksek bulunmuştur (P<0.05). In vitro sindirim sonrasında ekstraktların antioksidan aktivitesinde azalma meydana gelmiştir (P<0.05). Çekirdek ekstraktlarının sindirim öncesi ADE inhibisyon aktivitesi (%86.04±0.00), sindirim öncesi iç ekstraktın ADE inhibisyon aktivitesinden (%42.74±8.57) daha yüksektir (P<0.05). Çekirdek ve iç ekstraktların α-amilaz inhibisyon aktivitesi sırasıyla %49.18±0.35 ve %36.07±5.83 olarak belirlenmiştir. Çalışmanın sonuçlarına göre iç fraksiyonundan elde edilen ekstraktların ADE inhibisyon ve α-amilaz inhibisyon aktivitesi in vitro sindirimden sonra artmıştır.

Anahtar Kelimeler

• Ziziphus jujuba
• antioksidan aktivite
• ACE inhibisyon aktivite
• α-amilaz inhibisyon aktivite
• antosiyanin.

INVESTIGATION OF CHANGES IN SOME BIOACTIVE PROPERTIES OF PHENOLIC EXTRACTS FROM PULP AND SEED TISSUES OF ZIZIPHUS JUJUBA DURING IN VITRO DIGESTION

Öz

In this study, changes in angiotensin-I converting enzyme (ACE) inhibitory, α-amylase inhibitory and antioxidant activities, total phenolic content (TPC), total monomeric anthocyanin content (TMAC) of ultrasonic phenolic extracts from pulp and seed of Ziziphus jujuba were investigated during in vitro digestion. Bioaccessible fractions of phenolics in seed and pulp extracts were calculated as 23.24±4.46% and 9.43±0.24%, respectively. Moreover, bioaccessibility for TMAC in seed extracts (147.83±9.20%) was higher than pulp (15.76±3.89%) (P<0.05). A decrease in the antioxidant activity of the extracts occurred after in vitro digestion (P<0.05). The ACE inhibitory activity of undigested extracts from seed (86.04±0.00%) was higher than that of the undigested pulp extract (42.74±8.57%) (P<0.05). The α-amylase inhibitory activity of seed and pulp extracts was determined as 49.18±0.35% and 36.07±5.83%, respectively. The results of the study showed that ACE inhibitory activity and α-amylase inhibitory activity of the polyphenolics from pulp increased after in vitro digestion.

Anahtar Kelimeler

• Ziziphus jujuba
• antioxidant activity
• ACE inhibitory activity
• α-amylase inhibitory activity
• anthocyanin.

Kaynakça

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