FARKLI ÇÖZÜCÜLERİN TÜRKİYE`DE YETİŞEN CİSTUS CRETICUS EKSTRAKLARININ KİMYASAL KOMPOZİSYON, ANTİOKSİDAN AKTİVİTE VE ANTİMİKROBİYAL POTANSİYELİNE ETKİSİ

Öz

Bu çalışmanın amaçları (1) Türkiye`nin farklı bölgelerinden toplanan Cistus creticus L. ekstrelerinin verim (%), antioksidan ve antimikrobiyal aktivitelerini değerlendirmek, (2) metanol ve su çözücülerinin Cistus creticus L ekstrelerinin kimyasal kompozisyon, toplam fenolik ve flavanoid miktarlarına, IC50 ve FRAP değerlerine etkisini araştırmaktır. Ekstreler zayıf bir antimikrobiyal etki gösterirken, antioksidan deneylerinin sonuçları çok umut vericidir. IC50 değerleri metanol ve su ekstreleri için sırasıyla 13.94 µg/mL ve 34.41 µg/mL'dir. Benzer olarak, metanol ekstresinin FRAP değeri (1.27 M/g) su ekstresininkinden (0.72 M/g) daha yüksek çıkmıştır. HPLC analizinin sonuçları rutinin sadece (%13.252) metanol ekstresinde olduğunu göstermektedir. Ayrıca, metanol ekstresi daha yüksek miktarda kuersetin (%7.909), benzoik asit (%4.226) ve klorojenik asit (%2.168) içerirken, buna karşın su ekstresi daha yüksek miktarda gallik asit değerine (%13.705) sahiptir.

Anahtar Kelimeler

• Bitki ekstresi
• Antioksidan aktivite
• Biyoaktif maddeler
• Flavanoidler
• Fenolikler

THE EFFECT OF DIFFERENT SOLVENTS ON CHEMICAL COMPOSITION, ANTIOXIDANT ACTIVITY, AND ANTIMICROBIAL POTENTIAL OF TURKISH CISTUS CRETICUS EXTRACTS

Öz

The aims of this study were (1) to assess the extract yield, antioxidant activity, and antimicrobial activity of Cistus creticus extracts obtained from different locations in Türkiye (2) to evaluate the effect of water and methanol solvents on chemical composition, total polyphenol and flavonoid content, IC50, and FRAP values of C. creticus. The extracts had a weak inhibitory effect against tested microorganisms. However, the results of antioxidant assays were very promising. The IC50 values of methanol and water extracts were 13.94 µg/mL and 34.41 µg/mL, respectively. Similarly, the FRAP value of methanolic extract (1.27 M/g) was higher than the water extract (0.72 M/g). The results of HPLC analysis demonstrated that rutin was found only in the methanol extract (13.252%). In addition, the methanol extract had a higher content of quercetin (7.909%), benzoic acid (4.226%), and chlorogenic acid (2.168%), whereas the water extract possessed a higher amount of gallic acid (13.705%).

Anahtar Kelimeler

• Plant extract
• Antioxidant activity
• Bioactive substances
• Flavanoids
• Phenolics

Kaynakça

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