KOMBU ÇAYI ÜRETİMİNDE MUŞMULA (MESPILUS GERMANICA L.) KULLANIMININ ANTİOKSİDAN KAPASİTE VE BİYOERİŞİLEBİLİRLİK ÜZERİNE ETKİSİNİN ARAŞTIRILMASI
Yıl 2023,
, 1021 - 1035, 15.10.2023
Elif Yıldız
,
Gülşah Özcan Sinir
,
Didem Peren Aykas
Ozan Gürbüz
Öz
Kombu çayı, Kombu çayı kültüründe yer alan asetik asit bakterileri ve mayaların simbiyotik ilişkisi ile meydana gelen asetik asit ve alkol fermantasyonu ile karakterize olan ekşimsi-tatlımsı bir içecektir. Bu çalışmada sağlık açısından birçok faydası bulunan Kombu çayının biyoaktif potansiyelinin arttırılması hedeflenmiştir. Muşmula (Mespilus germanica L.) meyvesinin farklı oranlarda (%5, %10 ve %20) yeşil çaya katılmasını takiben gerçekleşen fermantasyon ile Kombu çayı hazırlanmıştır. Bu çaylar, antioksidan kapasite, toplam fenolik madde miktarı ve biyoerişilebilirlik açısından değerlendirilmiştir. Kombu çayı örneklerine ilave edilen muşmula oranının artması ile biyoaktif potansiyelin yükseldiği gözlemlenmiştir. %20 oranında muşmula ilavesi ile biyoerişilebilir fenolik fraksiyonların DPPH metoduna göre antioksidan kapasitesi 26.21±0.25 μmol TE/mL’den 38.54±0.08 μmol TE/mL’ye, toplam fenolik bileşen içeriği ise 42.83±1.02 mg GAE/100 mL’den 58.13±0.64 mg GAE/100 mL’ye yükselmiştir. Muşmula meyvesi ilavesi ile hazırlanan Kombu çayı hem yüksek biyoaktif içeriğe sahip bir içecek ortaya çıkartmış hem de yabani olarak yetişmekte olan muşmula meyvesinin endüstriyel kullanım alanını arttırma potansiyeli oluşturmuştur.
Kaynakça
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- Akçay, M.E., Özdemir, Y., Doğan, A. (2016). Determination of some characteristics of Akçakoca 77® a new cultiard for medlar cultivation. Bahçe Special, 145: 832-837.
- Al-Mohammadi, A.R., Ismaiel, A.A., Ibrahim, R.A., Moustafa, A.H., Abou Zeid, A., Enan, G. (2021). Chemical constitution and antimicrobial activity of kombucha fermented beverage. Molecules, 26(16): 5026. https://doi.org/10.3390/molecules26165026
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- Bouayed J., Deußer H., Hoffmann L., Bohn T. (2012). Bioaccessible and Dialysable Polyphenols in Selected Apple Varieties Following In Vitro Digestion vs. Their Native Patterns. Food Chemistry, 131(4): 1466-1472. https://doi.org/ 10.1016/j.foodchem.2011.10.030
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- Degirmencioglu, N., Yildiz, E., Sahan, Y., Guldas, M., Gurbuz, O. (2021). Impact of tea leaves types on antioxidant properties and bioaccessibility of kombucha. Journal of Food Science and Technology, 58(6): 2304-2312. https://doi.org/10.1007/ s13197-020-04741-7
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- Farhad, M., Kailasapathy, K., Tamang, J.P. (2010). Health aspects of fermented foods. In: Fermented foods and beverages of the world, Prakash Tamang J., Kasipathy K. (ed.), CRC Press, Boca Raton, pp. 391-414.
- Giritlioğlu, N., Yıldız, E., Gürbüz, O. (2020). Kombu Çayı Üretiminde Kapari Tomurcuklarının (Capparis spp.) Kullanımının Fenolikler, Antioksidant Kapasite ve Biyoerişilebilirliğe Etkisi. Akademik Gıda, 18(4): 390-401.
- Glew, R.H., Ayaz, F.A., Sanz, C., VanderJagt, D.J., Huang, H.S., Chuang, L.T., Strnad, M. (2003). Effect of postharvest period on sugars, organic acids and fatty acids composition in commercially sold medlar (Mespilus germanica'Dutch') fruit. European Food Research and Technology, 216: 390-394. https://doi.org/ 10.1007/s00217-002-0654-3
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- Ivanišová, E., Meňhartová, K., Terentjeva, M., Harangozo, Ľ., Kántor, A., Kačániová, M. (2020). The evaluation of chemical, antioxidant, antimicrobial and sensory properties of kombucha tea beverage. Journal of Food Science and Technology, 57, 1840-1846. https://doi.org/ 10.1007/s13197-019-04217-3
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INVESTIGATION OF THE EFFECT OF MEDLAR (MESPILUS GERMANICA L.) USAGE ON ANTIOXIDANT CAPACITY AND BIOACCESSIBILITY IN KOMBUCHA PRODUCTION
Yıl 2023,
, 1021 - 1035, 15.10.2023
Elif Yıldız
,
Gülşah Özcan Sinir
,
Didem Peren Aykas
Ozan Gürbüz
Öz
Kombucha is a sour-sweet beverage that is characterized by acetic acid and alcohol fermentation that occurs with the symbiotic relationship of acetic acid bacteria and yeasts in the kombucha culture. This study aimed to increase the bioactive potential of Kombucha, which has many health benefits. Kombucha was prepared by fermentation following the addition of medlar (Mespilus germanica L.) fruit to green tea at different rates (5%, 10%, and 20%). These teas were evaluated regarding antioxidant capacity, total phenolic content, and bioaccessibility. It was observed that the bioactive potential increased with the increase in the ratio of medlar added to the Kombucha samples. With the addition of medlar at the level of 20%, the antioxidant capacity of bioaccessible phenolic fractions for the DPPH method increased from 26.21±0.25 μmol TE/mL to 38.54±0.08 μmol TE/mL, and the total phenolic content increased from 42.83±1.02 mg GAE/100 mL to 42.83±1.02 mg GAE/100 mL. Kombucha prepared with the addition of medlar fruit has revealed a beverage with high bioactive content and also demonstrated the potential to increase the industrial use of the wild medlar fruit.
Kaynakça
- Abaci, N., Deniz, F.S.S., Orhan, I.E. (2022). Kombucha-An ancient fermented beverage with desired bioactivities: A narrowed review. Food Chemistry: X, 14: 100302. https://doi.org/ 10.1016/j.fochx.2022.100302
- Akçay, M.E., Özdemir, Y., Doğan, A. (2016). Determination of some characteristics of Akçakoca 77® a new cultiard for medlar cultivation. Bahçe Special, 145: 832-837.
- Al-Mohammadi, A.R., Ismaiel, A.A., Ibrahim, R.A., Moustafa, A.H., Abou Zeid, A., Enan, G. (2021). Chemical constitution and antimicrobial activity of kombucha fermented beverage. Molecules, 26(16): 5026. https://doi.org/10.3390/molecules26165026
- Anson, N.M., Selinheimo, E., Havenaar, R., Aura, A.M., Mattila, I., Lehtinen, P., Bast, A., Poutanen, K., Haenen, G.R. (2009). Bioprocessing of wheat bran improves in vitro bioaccessibility and colonic metabolism of phenolic compounds. Journal of Agricultural and Food Chemistry, 57(14): 6148-6155. https://doi.org/10.1021/jf900492h
- AOAC. (1990). Official Methods of Analysis. Maryland, USA: Association of Official Analytical, Chemists International.
- Apak, R., Guclu, K., Ozyurek, M., Celik, S.E. (2008). Mechanism of Antioxidant Capacity Assays and the CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Assay. Microchimica Acta, 160(4): 413-419. https://doi.org/10.1007/ s00604-007-0777-0
- Battikh, H., Bakhrouf, A., Ammar, E. (2012). Antimicrobial effect of Kombucha analogues. LWT-Food Science and Technology, 47(1): 71-77. https://doi.org/10.1016/ j.lwt.2011.12.033
- Benzie, I.F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘‘Antioxidant Power’’: The FRAP assay. Analytical Biochemistry, 239: 70-76. https://doi.org/ 10.1006/abio.1996.0292
- Bhattacharya, D., Bhattacharya, S., Patra, M.M., Chakravorty, S., Sarkar, S., Chakraborty, W., Koley, H., Gachhui, R. (2016). Antibacterial activity of polyphenolic fraction of kombucha against enteric bacterial pathogens. Current Microbiology, 73: 885-896. https://doi.org/ 10.1007/s00284-016-1136-3
- Bishop, P., Pitts, E.R., Budner, D., Thompson-Witrick, K.A. (2022). Chemical composition of kombucha. Beverages, 8(3): 45. https://doi.org/ 10.3390/beverages8030045
- Bouayed J., Deußer H., Hoffmann L., Bohn T. (2012). Bioaccessible and Dialysable Polyphenols in Selected Apple Varieties Following In Vitro Digestion vs. Their Native Patterns. Food Chemistry, 131(4): 1466-1472. https://doi.org/ 10.1016/j.foodchem.2011.10.030
- Brand-Williams W., Cuvelier M.E., Berset, C. (1995). Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT - Food Science and Technology, 28(1): 25-30. https://doi.org/10.1016/ S0023-6438(95)80008-5
- Cardoso, R.R., Neto, R.O., dos Santos D'Almeida, C.T., do Nascimento, T.P., Pressete, C.G., Azevedo, L., Martino, H.S.D., Cameron, L.C., Ferreira, M.S.L., de Barros, F.A.R. (2020). Kombuchas from green and black teas have different phenolic profile, which impacts their antioxidant capacities, antibacterial and antiproliferative activities. Food Research International, 128: 108782. https://doi.org/ 10.1016/j.foodres.2019.108782
- Chang, M.Y., Lin, Y.Y., Chang, Y.C., Huang, W.Y., Lin, W.S., Chen, C.Y., Huang, S.L., Lin, Y.S. (2020). Effects of infusion and storage on antioxidant activity and total phenolic content of black tea. Applied Sciences, 10(8): 2685. https://doi.org/10.3390/app10082685
- Chu, S.C., Chen, C. (2006). Effects of origins and fermentation time on the antioxidant activities of kombucha. Food Chemistry, 98(3): 502-507. https://doi.org/10.1016/j.foodchem.2005.05.080
- da Silva Júnior, J.C., Mafaldo, Í.M., de Lima Brito, I., de Magalhães Cordeiro, A.M.T. (2022). Kombucha: Formulation, chemical composition, and therapeutic potentialities. Current Research in Food Science, 5: 360-365. https://doi.org/ 10.1016/j.crfs.2022.01.023
- Degirmencioglu, N., Yildiz, E., Guldas, M., Gurbuz, O. (2020). Health benefits of kombucha tea enriched with olive leaf and honey. Journal of Obesity and Chronic Diseases, 4(1): 1-5. https://doi.org/10.17756/jocd.2020-031
- Degirmencioglu, N., Yildiz, E., Sahan, Y., Guldas, M., Gurbuz, O. (2021). Impact of tea leaves types on antioxidant properties and bioaccessibility of kombucha. Journal of Food Science and Technology, 58(6): 2304-2312. https://doi.org/10.1007/ s13197-020-04741-7
- Durul, M.S., Unver, H. (2016). Morphological and chemical properties of medlar (Mespilus germanica L.) fruits and changes in quality during ripening. Agrofor, 1(2): 133-140.
- Ercisli, S., Sengul, M., Yildiz, H., Sener, D., Duralija, B., Voca, S., Purgar, D.D. (2012). Phytochemical and antioxidant characteristics of medlar fruits (Mespilus germanica L.). Journal of Applied Botany and Food Quality, 85(1): 86.
- Erdoğan, A., Gürses, M., Güngör Sat, İ. (2006). Effects of the different temperature and saccharose concentrations on some microbiological and chemical characteristics of medlar pickle. International Journal of Food Properties, 9(2): 179-184. https://doi.org/10.1080/ 10942910600596183
- Essawet, N.A., Cvetković, D., Velićanski, A., Čanadanović-Brunet, J., Vulić, J., Maksimović, V., Markov, S. (2015). Polyphenols and antioxidant activities of Kombucha beverage enriched with Coffeeberry® extract. Chemical Industry and Chemical Engineering Quarterly, 21(3): 399-409. https://doi.org/10.2298/CICEQ140528042E
- Farhad, M., Kailasapathy, K., Tamang, J.P. (2010). Health aspects of fermented foods. In: Fermented foods and beverages of the world, Prakash Tamang J., Kasipathy K. (ed.), CRC Press, Boca Raton, pp. 391-414.
- Giritlioğlu, N., Yıldız, E., Gürbüz, O. (2020). Kombu Çayı Üretiminde Kapari Tomurcuklarının (Capparis spp.) Kullanımının Fenolikler, Antioksidant Kapasite ve Biyoerişilebilirliğe Etkisi. Akademik Gıda, 18(4): 390-401.
- Glew, R.H., Ayaz, F.A., Sanz, C., VanderJagt, D.J., Huang, H.S., Chuang, L.T., Strnad, M. (2003). Effect of postharvest period on sugars, organic acids and fatty acids composition in commercially sold medlar (Mespilus germanica'Dutch') fruit. European Food Research and Technology, 216: 390-394. https://doi.org/ 10.1007/s00217-002-0654-3
- Hur, S.J., Lee, S.Y., Kim, Y.C., Choi, I., Kim, G.B. (2014). Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry, 160: 346-356. https://doi.org/10.1016/ j.foodchem.2014.03.112
- Ivanišová, E., Meňhartová, K., Terentjeva, M., Harangozo, Ľ., Kántor, A., Kačániová, M. (2020). The evaluation of chemical, antioxidant, antimicrobial and sensory properties of kombucha tea beverage. Journal of Food Science and Technology, 57, 1840-1846. https://doi.org/ 10.1007/s13197-019-04217-3
- Jakubczyk, K., Kałduńska, J., Kochman, J., Janda, K. (2020). Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants, 9(5): 447. https://doi.org/10.3390/antiox9050447
- Jarrell, J., Cal, T., Bennett, J.W. (2000). The Kombucha consortia of yeasts and bacteria. Mycologist, 14(4): 166-170. https://doi.org/ 10.1016/S0269-915X(00)80034-8
- Jayabalan, R., Malbaša, R.V., Lončar, E.S., Vitas, J.S., Sathishkumar, M. (2014). A review on kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4): 538-550. https://doi.org/ 10.1111/1541-4337.120
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