BROİLER ETLERİNDEKİ TETRASİKLİN VE FLORFENİKOL ANTİBİYOTİK KALINTILARININ ELISA İLE GÖRÜNTÜLENMESİ VE SIVI KROMATOGRAFİSİ KÜTLE/ KÜTLE SPEKTROMETRESİ İLE DOĞRULANMASI

Öz

Bu çalışmada Aydın ilinde tüketime sunulan broiler etlerindeki tetrasiklin ve florfenikol antibiyotik kalıntılarının araştırılması amaçlanmıştır. Bu amaçla farklı satış yerlerinden farklı zamanlarda toplanan 80 adet broiler örneği kullanılmıştır. Antibiyotik kalıntı varlığı ELISA test kitleriyle belirlenmiş ve LC-MS/MS cihazı ile doğrulama yapılmıştır. ELISA test sonuçlarına göre incelenen örneklerin 24’ünün (%30) tetrasiklin pozitif olduğu ve örneklerin hiçbirinde florfenikole rastlanılmadığı gözlenmiştir. Tetrasiklin içeren ve LC-MS/MS’de doğrulaması yapılan 24 örnekteki antibiyotik düzeyi ortalama 30 ppm olarak bulunmuştur. Bulunan değerin maksimum kalıntı düzeyinin (MRL) altıda olduğu görülmüştür.

Anahtar Kelimeler

• tetrasiklin,ELISA,Florfenikol,LC-MS/MS

SCREENING OF TETRACYCLINE AND FLORFENICOL ANTIBIOTIC RESIDUES IN BROILER MEAT USING ELISA AND CONFIRMATION BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY

Öz

The aim of this study was to investigate the presence of tetracycline and florfenicol residues in broiler meat that sailed in Aydın city, Turkey. For this purpose, 80 broiler meats that brands of local and national commercial were used. ELISA technique was used to investigate the presence of antibiotic residues and liquid chromatography/ tandem mass spectrometry (LC-MS/MS) technique was used to confirm the residue. As result of the ELISA, 24 samples 30%) were positive for tetracycline. Florfenicol residues were found in any of the samples. 24 samples that is confirmed by LC-MS/MS were included an average of 30 ppb level. Residual amounts of the samples were found to be below the maximum residue limit (MRL) values. 

Anahtar Kelimeler

• Florfenicol,tetracycline,ELISA,LC-MS/MS

Kaynakça

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