Araştırma Makalesi
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DETERMINATION OF INTERACTIONS BETWEEN MEAT PROTEINS AND CARRAGEENANS WITH DIFFERANTIAL SCANNIND CALORIMETRY

Yıl 2018, , 776 - 786, 01.10.2018
https://doi.org/10.15237/gida.GD18061

Öz

In this study the interactions
between meat proteins and κ, ι, λ-carrageenans with or without NaCl were
investigated. For this purpose the thermal denaturation (T
p) and
glass transition temperatures (T
g) and gelation temperatures of
carrageenans were determined by using differential scanning calorimetry (DSC).
Three denaturation peaks were found for ground meat which were 57.96
°C for myosin, 66.08°C for sarcoplasmic proteins and
79.23
°C for actin. The Tp of
myosin and actin were decreased while T
p of sarcoplasmic proteins
were increased when NaCl was added. Addition of carrageenans decreased the T
p
of actin and myosin and it was observed that effect of carrageenans was
increased when salt was added. The T
g was observed as step change in
the thermograms. Addition of salt did not affect T
g of samples, only
ι-carrageenan increased T
g of samples (P ≤0.05). On the other hand κ, λ-carrageenan caused a decrease in
the T
g of samples (P >0.05). 

Kaynakça

  • Akkose, A., Aktas, N. (2008). Determination of glass transition temperature of beef and effects of various cryoprotective agents on some chemical changes. Meat Science. 80: 875–878.
  • Amako, D.E.N., Xiong, Y.L. (2001). Effects of carrageenan on thermal stability of proteins from chicken thigh and breast muscles. Food Research International. 34: 247-253.
  • Candogan, K., Kolsarici, N. (2003). The effects of carrageenan and pectin on some quality characteristics of low-fat beef frankfurters. Meat Science. 64: 199–206.
  • Chen, H.H., Xu, S.Y., Wang, Z. (2007). Interaction between flaxseed gum and meat protein, Journal of Food Engineering. 80: 1051-1059.
  • DeFreitas, Z., Sebranek, J.G., Olson, D.G., Carr, J.M. (1997). Carrageenan effects on thermal stability of meat proteins. Journal of Food Science. 62(3): 544-547.
  • Dickensen, E., (2003) Hydrocolloids at interfaces and the influence on the properties of dispersed systems, Food Hydrocolloids. 17: 25-39.
  • Harwalker U.R., Ma, C.Y. (Ed.), Findlay, C.J., Barbut, S. (1990). Thermal analysis of meat. Ch. 4, In Thermal Analysis of Food. Elsevier Applied Science, New York. p. 93–125.
  • Li, Y.T., Jiang, Y.M. (2004). Carrageenan and cooked meat products. Journal Meat. 9: 46–47.
  • Ma, C. Y., Harwalkar V. R., Maurice, T. J., (1990). Instrumentation and Techniques of Thermal Analysis in Food Research, In “Thermal Analysis of Food” p: 1-16.
  • Orlien, V., Anderson, M. L., Jouhtimaki, S., Risbo, J., & Skibsted, L. H. (2004). Effect of temperature and glassy states on the molecular mobility of solutes in frozen tuna muscle as studied by electron spin resonance spectroscopy with spin probe detection. Journal of Agricultural and Food Chemistry. 52: 2269–2276.
  • Pighin, D.G., Sancho, A.M., Gonzalez, C.B. (2008). Effect of salt addition on the thermal behavior of proteins of bovine meat from Argentina. Meat Science, 79: 549–556.
  • Shand, P.J., Sofos, J.N., and Schmidt, G.R. (1994). Differential scanning calorimetry of beef/kappa-carrageenan mixtures. Journal of Food Science. 59: 711–715.
  • Sunooj, K.V., Radhakrishna, K., George, J., Bawa, A.S. (2009). Factors influencing the calorimetric determination of glass transition temperature in foods: A case study using chicken and mutton. Journal of Food Engineering. 91: 347–352.
  • Tomšic, M., Prossnigg, F., Glatter, O., (2008) A thermoreversible double gel: Characterization of a methylcellulose and κ-carrageenan mixed system in water by SAXS, DSC and rheology, Journal of Colloid and Interface Science. 322: 41–50.
  • Towle, G.A. (1973). Carrageenan. Ch. 5 In Industrial Gums, Polysaccharides and Their Derivatives. 2nd Edition, Academic Press. New York p. 83–114.
  • Voutila, L., Ruusunen, M., Puolanne, E. (2008). Comparison of the thermal characteristics of connective tissue in loose structured and normal structured porcine M. Semimembranosus. Meat Science. 80: 1024-1030.
  • Wright, D.J., Leach, I.B., and Wilding, P. (1977). Differential scanning calorimetric studies of muscle and its constituent proteins. J. Sci. Food Agric. 28: 557–564.
  • Zhou, W.W., Meng, L., Li, X., Ma, L., Dai, R. (2010). Effect of the intereaction between carrageenan, gellan gum and flaxseed gum on quality attributes of strach-free emulsion-type sausage. Journal of Muscle Foods. 21: 255-267.

KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ

Yıl 2018, , 776 - 786, 01.10.2018
https://doi.org/10.15237/gida.GD18061

Öz

Bu çalışma kapsamında et proteinlerinin farklı
karragenanlar ile etkileşimi NaCl eklendiği ve eklenmediği durumlarda
araştırılmıştır. Bu amaçla et proteinlerinin denatürasyon pik sıcaklıkları (T
p)
ve camsı geçiş sıcaklıkları (T
g) ve karragenanların jelleşme
sıcaklıkları differansiyel taramalı kalorimetre (DTK) ile incelenmiştir.
DTK’dan elde edilen termogramlarda miyosin için 57.96
°C, sarkoplazmik proteinler için 66.08°C ve aktin için 79.23°C olmak üzere 3 temel Tp değeri bulunmuştur.
Kıyma haline getirilmiş dana eti tuz eklendiğinde miyosin ve aktinin T
p
değerlerinde azalma sarkoplazmik proteinlerininkinde ise artış gözlenmiştir.
Karragenanlar eklendiğinde ise proteinlerin aktin ve miyosinin T
p
değerlerinde azalma gözlenmiştir. Karragenanlar etkilerini tuz varlığında daha
belirgin olarak göstermişlerdir (
P ≤0.05).
Camsı geçiş sıcaklığı DTK termogramlarında basamak değişim olarak gözlenmiştir.
Tuz eklemenin camsı geçiş sıcaklığı üzerine önemli bir etkisi gözlenmezken (
P >0.05), eklenen karragenanlardan
yalnızca ι-karragenan artışa neden olmuştur (
P ≤0.05). κ ve λ-karragenan ise camsı geçiş sıcaklıklarında
azalmaya neden olmuşlardır (
P >0.05). 

Kaynakça

  • Akkose, A., Aktas, N. (2008). Determination of glass transition temperature of beef and effects of various cryoprotective agents on some chemical changes. Meat Science. 80: 875–878.
  • Amako, D.E.N., Xiong, Y.L. (2001). Effects of carrageenan on thermal stability of proteins from chicken thigh and breast muscles. Food Research International. 34: 247-253.
  • Candogan, K., Kolsarici, N. (2003). The effects of carrageenan and pectin on some quality characteristics of low-fat beef frankfurters. Meat Science. 64: 199–206.
  • Chen, H.H., Xu, S.Y., Wang, Z. (2007). Interaction between flaxseed gum and meat protein, Journal of Food Engineering. 80: 1051-1059.
  • DeFreitas, Z., Sebranek, J.G., Olson, D.G., Carr, J.M. (1997). Carrageenan effects on thermal stability of meat proteins. Journal of Food Science. 62(3): 544-547.
  • Dickensen, E., (2003) Hydrocolloids at interfaces and the influence on the properties of dispersed systems, Food Hydrocolloids. 17: 25-39.
  • Harwalker U.R., Ma, C.Y. (Ed.), Findlay, C.J., Barbut, S. (1990). Thermal analysis of meat. Ch. 4, In Thermal Analysis of Food. Elsevier Applied Science, New York. p. 93–125.
  • Li, Y.T., Jiang, Y.M. (2004). Carrageenan and cooked meat products. Journal Meat. 9: 46–47.
  • Ma, C. Y., Harwalkar V. R., Maurice, T. J., (1990). Instrumentation and Techniques of Thermal Analysis in Food Research, In “Thermal Analysis of Food” p: 1-16.
  • Orlien, V., Anderson, M. L., Jouhtimaki, S., Risbo, J., & Skibsted, L. H. (2004). Effect of temperature and glassy states on the molecular mobility of solutes in frozen tuna muscle as studied by electron spin resonance spectroscopy with spin probe detection. Journal of Agricultural and Food Chemistry. 52: 2269–2276.
  • Pighin, D.G., Sancho, A.M., Gonzalez, C.B. (2008). Effect of salt addition on the thermal behavior of proteins of bovine meat from Argentina. Meat Science, 79: 549–556.
  • Shand, P.J., Sofos, J.N., and Schmidt, G.R. (1994). Differential scanning calorimetry of beef/kappa-carrageenan mixtures. Journal of Food Science. 59: 711–715.
  • Sunooj, K.V., Radhakrishna, K., George, J., Bawa, A.S. (2009). Factors influencing the calorimetric determination of glass transition temperature in foods: A case study using chicken and mutton. Journal of Food Engineering. 91: 347–352.
  • Tomšic, M., Prossnigg, F., Glatter, O., (2008) A thermoreversible double gel: Characterization of a methylcellulose and κ-carrageenan mixed system in water by SAXS, DSC and rheology, Journal of Colloid and Interface Science. 322: 41–50.
  • Towle, G.A. (1973). Carrageenan. Ch. 5 In Industrial Gums, Polysaccharides and Their Derivatives. 2nd Edition, Academic Press. New York p. 83–114.
  • Voutila, L., Ruusunen, M., Puolanne, E. (2008). Comparison of the thermal characteristics of connective tissue in loose structured and normal structured porcine M. Semimembranosus. Meat Science. 80: 1024-1030.
  • Wright, D.J., Leach, I.B., and Wilding, P. (1977). Differential scanning calorimetric studies of muscle and its constituent proteins. J. Sci. Food Agric. 28: 557–564.
  • Zhou, W.W., Meng, L., Li, X., Ma, L., Dai, R. (2010). Effect of the intereaction between carrageenan, gellan gum and flaxseed gum on quality attributes of strach-free emulsion-type sausage. Journal of Muscle Foods. 21: 255-267.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Esen Eyiler

Halil Vural

Yayımlanma Tarihi 1 Ekim 2018
Yayımlandığı Sayı Yıl 2018

Kaynak Göster

APA Eyiler, E., & Vural, H. (2018). KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ. Gıda, 43(5), 776-786. https://doi.org/10.15237/gida.GD18061
AMA Eyiler E, Vural H. KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ. GIDA. Ekim 2018;43(5):776-786. doi:10.15237/gida.GD18061
Chicago Eyiler, Esen, ve Halil Vural. “KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ”. Gıda 43, sy. 5 (Ekim 2018): 776-86. https://doi.org/10.15237/gida.GD18061.
EndNote Eyiler E, Vural H (01 Ekim 2018) KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ. Gıda 43 5 776–786.
IEEE E. Eyiler ve H. Vural, “KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ”, GIDA, c. 43, sy. 5, ss. 776–786, 2018, doi: 10.15237/gida.GD18061.
ISNAD Eyiler, Esen - Vural, Halil. “KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ”. Gıda 43/5 (Ekim 2018), 776-786. https://doi.org/10.15237/gida.GD18061.
JAMA Eyiler E, Vural H. KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ. GIDA. 2018;43:776–786.
MLA Eyiler, Esen ve Halil Vural. “KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ”. Gıda, c. 43, sy. 5, 2018, ss. 776-8, doi:10.15237/gida.GD18061.
Vancouver Eyiler E, Vural H. KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ. GIDA. 2018;43(5):776-8.

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