ERZURUM’DA SATIŞA SUNULAN TAVUK ETLERİNİN MİKROBİYOLOJİK KALİTESİ VE SALMONELLA SPP. VARLIĞININ BELİRLENMESİ, SALMONELLA SPP. İZOLATLARIN ANTİBİYOTİK DİRENCİ

Öz

Bu çalışma, Erzurum’da marketlerden toplanan 45 adet tavuk eti örneğinde (15’er ciğer, göğüs, baget) belirli mikrobiyolojik kalite karakteristikleri, immunomanyetik separasyon (IMS) ve geleneksel yöntem ile Salmonella spp. varlığını araştırmak için yapılmıştır. Ayrıca, Salmonella spp. izolatlarının antibiyotik dirençleri değerlendirilmiştir. Toplamda 15 örnekte IMS ve geleneksel metot ile Salmonella spp. pozitif bulunmuştur. Bu izolatlar API 20E biyokimyasal analiz kiti kullanılarak %99,9 ihtimalle doğrulanmıştır. Salmonella spp. izolatları siprofloksasin, kloromfenikol ve gentamisin antibiyotiklerine karşı %100 seviyesinde duyarlılık gösterirken, sırasıyla tetrasiklin, trimetoprim, sülfametoksazol/trimethoprim, nalidiksik asit, streptomisin, ampisilin ve kanamisin antibiyotiklerine karşı direnç göstermiştir. Elde edilen sonuçlara göre tavuk etlerinin %84’ü (38/45) toplam bakteri sayısı, %33’ünün (15/45) Salmonella spp. varlığı açısından Türk Gıda Kodeks’ine uygun olmadığı görülmüştür.

Anahtar Kelimeler

• Tavuk etleri,mikrobiyolojik kalite,Salmonella spp.,immunomanyetik separasyon,antibiyotik direnç

DETERMINATION OF MICROBIOLOGICAL QUALITY AND PRESENCE OF SALMONELLA SPP. ON CHICKEN PARTS SOLD AT RETAIL MARKETS IN ERZURUM, ANTIBIOTIC RESISTANCE OF THE SALMONELLA SPP. ISOLATES

Öz

The present study was conducted to investigate the certain microbiological quality characteristics, and presence of Salmonella spp. by immunomagnetic separation (IMS) and conventional method, also to evaluate the antibiotic resistance of Salmonella spp. isolates obtained from 45 chicken meat samples (fifteen livers, chests, baguettes) collected from markets at the Erzurum. Totally, in the 15 samples were found positive for Salmonella spp. by IMS and conventional methods. Isolates were identified with 99.9% probability using API 20E biochemical analysis kit. The results of antibiotic test showed the susceptibility of the isolates to ciprofloxacin, chloramphenicol and gentamicin at 100% level, while these isolates were resistance to tetracycline, trimethoprim, sulfamethoxazole/trimethoprim, nalidixic acid, streptomycin, ampicillin and kanamycin, respectively. The obtained results showed that 84% (38/45) of the chicken meat samples did not show suitability with Turkish Food Codex in terms of total bacteria count and presence of Salmonella in 33% (15/45) of the samples.

Anahtar Kelimeler

• Chicken meats,Microbiological quality,Salmonella spp.,Immunomagnetic separation (IMS),Antibitoic resistance

Kaynakça

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