Silybum marianum L. TOHUMLARININ YAĞ ASİDİ KOMPOZİSYONU İLE TOHUM YAĞI VE SİLYMARİN EKSTRAKTININ ANTİMİKROBİYAL ETKİSİ

Öz

Bu çalışmada Meryemana dikeni (Silybum marianum L.) tohumlarından elde edilen tohum sabit yağı ile metanol ekstraksiyonuyla elde edilen silmarin etken maddesinin antimikrobiyal aktivitesinin belirlenmesi amaçlanmıştır. Ekstrelerin; Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus pentosus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa ve Candida albicans mikroorganizmalarına karşı antimikrobiyal aktiviteleri belirlenmiştir. Bununla birlikte bitki tohumlarından elde edilen yağın, yağ asitleri kompozisyonunun belirlenmesi için Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) yöntemi kullanılmıştır. Yağın ana bileşenleri; oktadekadienoik asit (% 44.06), oktadekenoik asit (% 20.18), hekzadekanoik asit (% 14.63) ve oktadekanoik asit (% 7.75) olarak saptanmıştır. Çalışmamızda; tohum yağı ekstraktının P. mirabilis, P. aeruginosa ve Lactobacillus pentosus ELB41 üzerine; silmarinin ise Lactobacillus plantarum ELB75, Lactobacillus pentosus ELB37 ve B. subtilis’ e karşı antimikrobiyal etkisinin olduğu saptanmıştır.

Anahtar Kelimeler

• Silybum marianum L., meryemana dikeni, yağ asidi kompozisyonu, silymarin, antimikrobiyal aktivite

FATTY ACID COMPOSITION OF Silybum marianum L. SEEDS AND ANTIMICROBIAL ACTIVITY OF SEED OIL AND SILYMARIN EXTRACT

Öz

In this study, it was aimed to determine fatty acid profile and the antimicrobial activity of the seed oil obtained from the seed of Silybum marianum L. and silymarin which was extracted through methanol extraction of Silybum marianum L. seed. The antimicrobial activity of extracts was determined by testing against the microorganisms of Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus pentosus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Candida albicans. Gas Chromatography-Mass Spectrometry was used to determine the fatty acid profile of the seed oil. The main components of the seed oil were detected as octadecadienoic acid (44.06 %), octadecenoic acid (20.18 %), hexadecenoic acid (14.63 %) and octadecanoic acid (7.75 %). Seed oil revealed antimicrobial effect on P. mirabilis, P. aeruginosa and L. pentosus ELB41 and silymarin had antimicrobial effect on L. plantarum ELB75, L. pentosus ELB37 and B. subtilis.

Anahtar Kelimeler

• Silybum marianum L.
• Milk thistle
• Fatty acid composition
• Antimicrobial activity

Kaynakça

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