DETERMINATION OF FATTY ACID COMPOSITION AND BIOACTIVE PROPERTIES OF PUMPKIN SEED AND APRICOT KERNEL OILS
Yıl 2021,
, 608 - 620, 15.05.2021
İlkin Yücelşengün
,
Ersin Yücel
,
Gülden Kılıç
,
Berna Öztürk
Öz
In the study, fatty acid compositions and bioactive properties of fixed oils obtained from seeds of pumpkin grown in Ankara and kernels of apricot grown in Malatya were investigated. The main components in the composition of pumpkin seed and apricot kernel oils were identified as linoleic and oleic acids. The total phenolic contents of pumpkin seed and apricot kernel oils were determined as 123.60 and 86.75 mg GAE/100 g, respectively. According to DPPH method, the antioxidant activities of pumpkin seed and apricot kernel oils were determined as 46.53% and 39.61%, respectively, while the values were 74.73% and 49.05% according to ABTS method. The most sensitive microorganism against pumpkin seed oil was Escherichia coli O157:H7, while Listeria monocytogenes, Enterococcus feacalis and Salmonella Typhimurium were determined as the most sensitive microorganisms to apricot kernel oil. The results showed that fixed oils could be used as natural antioxidant products in the food industry.
Kaynakça
- Ababouch, L., Bouqartacha, F., Busta, F.F. (1994). Inhibition of Bacillus cereus spores and vegetative cells by fatty acids and glyceryl monododecanoate. Food Microbiol, 55: 327-336, doi: 10.1006/fmic.1994.1037.
- Adnan, M., Gul, S., Batool, S., Fatima, B., Rehman, A., Yaqoob, S., Shabir, H., Yousaf, T., Mussarat, S., Ali, N., Khan, S.N., Rahman, H., Aziz, M.A. (2017). A review on the ethnobotany, phytochemistry, pharmacology and nutritional composition of Cucurbita pepo L. The Journal of Phytopharmacology, 6(2): 133-139.
- Ali, S., Masud, T., Abbasi, K.S. (2011). Physico-chemical characteristics of apricot (Prunus armeniaca L.) grown in Northern Areas of Pakistan. Sci Hortic, 130(2): 386-392, doi: 10.1016/j.scienta.2011.05.040.
- Amiran, F., Shafaghat, A., Shafaghatlonbar, M. (2015). Omega-6 content, antioxidant and antimicrobial activities of hexanic extract from Prunus armeniaca L. kernel from North-West Iran. National Academy Science Letters, 38(2): 107-111, doi: 10.1007/s40009-014-0284-x.
- Badr, S.E., Shaaban, M., Elkholy, Y.M., Helal, M.H., Hamza, A.S., Masoud, M.S., El Safty, M.M. (2011). Chemical composition and biological activity of ripe pumpkin fruits (Cucurbita pepo L.) cultivated in Egyptian habitats. Nat Prod Res, 25(16): 1524-1539, doi: 10.1080/14786410903312991.
- Bardaa, S., Halima, N.B., Aloui, F., Mansour, R.B., Jabeur, H., Bouaziz, M., Sahnoun, Z. (2016). Oil from pumpkin (Cucurbita pepo L.) seeds: evaluation of its functional properties on wound healing in rats. Lipids in Health and Disease, 15(1): 1-12, doi: 10.1186/s12944-016-0237-0.
- Benalia, M., Djeridane, A., Gourine, N., Nia, S., Ajandouz, E., Yousfi, M. (2015). Fatty acid profile, tocopherols content and antioxidant activity of algerian pumpkin seeds oil (Cucurbita pepo L.). Med J Nutrition Metab, 8(1): 9-25, doi: 10.3233/mnm-140023.
- Coşkun, F. (2006). Gıdalarda bulunan doğal koruyucular. Gıda Teknolojileri Elektronik Dergisi, 2: 27-33.
- Dar, P., Farman, M., Dar, A., Khan, Z., Munir, R., Rasheed, A., Waqas, U. (2017). Evaluation of antioxidant potential and comparative analysis of antimicrobial activity of various extracts of Cucurbita pepo L. leaves. J Agric Sci Food Technol, 3: 103-109.
- Deng, Y., Yang, G., Yue, J., Qian, B., Liu, Z., Wang, D., Zhong, Y., Zhao, Y. (2014). Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control, 38: 184-191 doi: 10.1016/j.foodcont.2013.10.023.
- Dulf, F.V., Vodnar, D.C., Dulf, E.H., Pintea, A. (2017). Phenolic compounds, flavonoids, lipids and antioxidant potential of apricot (Prunus armeniaca L.) pomace fermented by two filamentous fungal strains in solid state system. Chem Cent J, 11(1): 1-10, doi: 10.1186/s13065-017-0323-z.
- El-Sayed, M.M., Hashash, M.M., Abdel-Hady, A.A., Abdel-Hady, H., Abdel-Lateef, E.E., Morsi, E.A. (2017). Total phenolic and flavonoid contents and antioxidant activity of Lantana camara and Cucurbita pepo (Squash) extracts as well as GC-MS analysis of Lantana camara essential oils. World J Pharm Res, 6(1): 137-153.
- Ghaffar, F., Kainat, B., Shah, H., Akram, M. (2018). Nutritional, physico-chemical, antimicrobial and antioxidant screening of seed and seed oil of Cucurbita pepo grown in Kpk, Pakistan. FUUAST Journal of Biology, 8(1): 41-48.
- Gohari, A.A., Farhoosh, R., Haddad, K.M. (2011). Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo subsp. pepo var. Styriaka) grown in Iran. J Agric Sci Technol, 13: 1053-1063.
- Gomaa, E.Z. (2013). In vitro antioxidant, antimicrobial, and antitumor activities of bitter almond and sweet apricot (Prunus armeniaca L.) kernels. Food Sci Biotechnol, 22(2): 455-463, doi: 10.1007/s10068-013-0101-1.
- Gupta, A., Sharma, P.C., Tilakratne, B.M.K.S., Verma, A.K. (2012). Studies on physico-chemical characteristics and fatty acid composition of wild apricot (Prunus armeniaca Linn.) kernel oil. Indian Journal of Natural Products and Resources, 3(3): 366-370.
- Gyawali, R., Ibrahim, S.A. (2014). Natural products as antimicrobial agents. Food Control, 46: 412-429, doi: 10.1016/j.foodcont.2014.05.047.
- Hashash, M.M., El-Sayed, M.M., Abdel-Hady, A.A., Hady, H.A., Morsi, E.A. (2017). Nutritional potential, mineral composition, and antioxidant activity Squash (Cucurbıta pepo L.) fruits grown in Egypt. Inflammation, 9(10): 11-12.
- Juhaimi, F.A., Özcan, M.M., Ghafoor, K., Babiker, E.E. (2018). The effect of microwave roasting on bioactive compounds, antioxidant activity and fatty acid composition of apricot kernel and oils. Food Chem, 243: 414-419, doi: 10.1016/j.foodchem.2017.09.100.
- Karaca, E., Aytaç, S. (2007). Yağ bitkilerinde yağ asitleri kompozisyonu üzerine etki eden faktörler. Anadolu Tarım Bilimleri Dergisi, 22(1): 123-131.
- Kasnak, C., Palamutoğlu, R. (2015). Doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri. Turkish Journal of Agriculture-Food Science and Technology, 3(5): 226-234, doi: 10.24925/turjaf.v3i5.226-234.171.
- Kiralan, M., Özkan, G., Bayrak, A., Ramadan, M.F. (2014). Physicochemical properties and stability of black cumin (Nigella sativa) seed oil as affected by different extraction methods. Ind Crop Prod, 57: 52-58, doi: 10.1016/j.indcrop.2014.03.026.
- Korekar, G., Stobdan, T., Arora, R., Yadav, A., Singh, S.B. (2011). Antioxidant capacity and phenolics content of apricot (Prunus armeniaca L.) kernel as a function of genotype. Plant Foods Hum Nutr, 66(4): 376-383, doi: 10.1007/s11130-011-0246-0.
- Lee, H.H., Ahn, J.H., Kwon, A.R., Lee, E.S., Kwak, J.H., Min, Y.H. (2014). Chemical composition and antimicrobial activity of the essential oil of apricot seed. Phytotherapy Res, 28(12): 1867-1872, doi: 10.1002/ptr.5219.
- Li, X.J., Li, Z.G., Wang, X., Han, J.Y., Zhang, B., Fu, Y.J., Zhao, C.J. (2016). Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect. Food Chem, 212: 403-410, doi: 10.1016/j.foodchem.2016.05.185.
- Manzoor, M., Anwar, F., Ashraf, M., Alkharfy, K.M. (2012). Physico-chemical characteristics of seed oils extracted from different apricot (Prunus armeniaca L.) varieties from Pakistan. Grasas Y Aceites, 63(2): 193-201, doi: 10.3989/gya.095011.
- Meru, G., Fu, Y., Leyva, D., Sarnoski, P., Yagiz, Y. (2018). Phenotypic relationships among oil, protein, fatty acid composition and seed size traits in Cucurbita pepo. Sci Hortic, 233: 47-53, doi: 10.1016/j.scienta.2018.01.030.
- Morittu, V.M., Musco, N., Mastellone, V., Bonesi, M., Britti, D., Infascelli, F., Loizzo, M.R., Tundis, R., Sicari, V., Tudisco, R., Lombardi, P. (2019). In vitro and in vivo studies of Cucurbita pepo L. flowers: chemical profile and bioactivity. Nat Prod Res, 30: 1-5, doi: 10.1080/14786419.2019.1672067.
- Nafis, A., Kasrati, A., Jamali, C. A., Custódio, L., Vitalini, S., Iriti, M., Hassani, L. (2020). A Comparative study of the in vitro antimicrobial and synergistic effect of essential oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with antimicrobial drugs: New approach for health promoting products. Antibiotics, 9(4): 140, doi: 10.3390/antibiotics9040140.
- Naik, D.G., Dandge, C.N., Rupanar, S.V. (2011). Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from Gymnema sylvestre R. Br. leaves. J Essent Oil Res, 23(3): 12-19, doi: 10.1080/10412905.2011.9700451.
- Nourmohammadi, E., SadeghiMahoonak, A., Alami, M., Ghorbani, M. (2017). Amino acid composition and antioxidative properties of hydrolysed pumpkin (Cucurbita pepo L.) oil cake protein. Int J Food Prop, 20(12): 3244-3255, doi: 10.1080/10942912.2017.1283516.
- Preuss, H.G., Echard, B., Enig, M., Brook, I., Elliott, T.B. (2005). Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram-positive and gram-negative bacteria. Mol Cell Biochem, 272(1-2): 29-34, doi: 10.1007/s11010-005-6604-1.
- Proestos, C., Boziaris, I.S., Nychas, G.J.E., Komaitis, M. (2006). Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chem, 95: 664-671, doi: 10.1016/j.foodchem.2005.01.049.
- Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26: 1231-1237, doi: 10.1016/s0891-5849(98)00315-3.
- Sener, B., Orhan, I., Ozcelik, B., Kartal, M., Aslan, S., Ozbilen, G. (2007). Antimicrobial and antiviral activities of two seed oil samples of Cucurbita pepo L. and their fatty acid analysis. Natural Product Communications, 2(4): 395-398.
- Sharma, S., Satpathy, G., Gupta, R.K. (2014). Nutritional, phytochemical, antioxidant and antimicrobial activity of Prunus armenicus. J Pharmacogn Phytochem: 3(3), 23-28.
- Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult, 16: 144-158.
- Sójka, M., Kołodziejczyk, K., Milala, J., Abadias, M., Viñas, I., Guyot, S., Baron, A. (2015). Composition and properties of the polyphenolic extracts obtained from industrial plum pomaces. J Funct Foods, 12: 168-178, doi: 10.1016/j.jff.2014.11.015.
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KABAK VE KAYISI ÇEKİRDEĞİ YAĞLARININ YAĞ ASİDİ KOMPOZİSYONU, BİYOAKTİF ÖZELLİKLERİNİN BELİRLENMESİ
Yıl 2021,
, 608 - 620, 15.05.2021
İlkin Yücelşengün
,
Ersin Yücel
,
Gülden Kılıç
,
Berna Öztürk
Öz
Bu çalışmada, Ankara’da yetiştirilen kabak çekirdeği ve Malatya’da yetiştirilen kayısı çekirdeğinden elde edilen sabit yağların yağ asidi kompozisyonları ve biyoaktif özellikleri incelenmiştir. Kabak ve kayısı çekirdeği yağlarının bileşiminde bulunan ana bileşenler linoleik ve oleik asit olarak tespit edilmiştir. Kabak ve kayısı çekirdeği yağlarının toplam fenolik madde miktarı sırasıyla 123.60 ve 86.75 mg GAE/100 g olarak belirlenmiştir. DPPH yöntemine göre kabak ve kayısı çekirdeği yağlarının antioksidan aktivite değerleri sırasıyla %46.53 ve %39.61 iken ABTS yöntemine göre değerler %74.73 ve %49.05 olarak tespit edilmiştir. Kabak çekirdeği yağına karşı en hassas mikroorganizma Escherichia coli O157:H7 olarak tespit edilirken, Listeria monocytogenes, Enterococcus feacalis ve Salmonella Typhimurium, kayısı çekirdeği yağlarına karşı en hassas mikroorganizmalar olarak belirlenmiştir. Sonuçlar, sabit yağların gıda endüstrisinde doğal antioksidan ürünler olarak kullanılabileceğini göstermiştir.
Kaynakça
- Ababouch, L., Bouqartacha, F., Busta, F.F. (1994). Inhibition of Bacillus cereus spores and vegetative cells by fatty acids and glyceryl monododecanoate. Food Microbiol, 55: 327-336, doi: 10.1006/fmic.1994.1037.
- Adnan, M., Gul, S., Batool, S., Fatima, B., Rehman, A., Yaqoob, S., Shabir, H., Yousaf, T., Mussarat, S., Ali, N., Khan, S.N., Rahman, H., Aziz, M.A. (2017). A review on the ethnobotany, phytochemistry, pharmacology and nutritional composition of Cucurbita pepo L. The Journal of Phytopharmacology, 6(2): 133-139.
- Ali, S., Masud, T., Abbasi, K.S. (2011). Physico-chemical characteristics of apricot (Prunus armeniaca L.) grown in Northern Areas of Pakistan. Sci Hortic, 130(2): 386-392, doi: 10.1016/j.scienta.2011.05.040.
- Amiran, F., Shafaghat, A., Shafaghatlonbar, M. (2015). Omega-6 content, antioxidant and antimicrobial activities of hexanic extract from Prunus armeniaca L. kernel from North-West Iran. National Academy Science Letters, 38(2): 107-111, doi: 10.1007/s40009-014-0284-x.
- Badr, S.E., Shaaban, M., Elkholy, Y.M., Helal, M.H., Hamza, A.S., Masoud, M.S., El Safty, M.M. (2011). Chemical composition and biological activity of ripe pumpkin fruits (Cucurbita pepo L.) cultivated in Egyptian habitats. Nat Prod Res, 25(16): 1524-1539, doi: 10.1080/14786410903312991.
- Bardaa, S., Halima, N.B., Aloui, F., Mansour, R.B., Jabeur, H., Bouaziz, M., Sahnoun, Z. (2016). Oil from pumpkin (Cucurbita pepo L.) seeds: evaluation of its functional properties on wound healing in rats. Lipids in Health and Disease, 15(1): 1-12, doi: 10.1186/s12944-016-0237-0.
- Benalia, M., Djeridane, A., Gourine, N., Nia, S., Ajandouz, E., Yousfi, M. (2015). Fatty acid profile, tocopherols content and antioxidant activity of algerian pumpkin seeds oil (Cucurbita pepo L.). Med J Nutrition Metab, 8(1): 9-25, doi: 10.3233/mnm-140023.
- Coşkun, F. (2006). Gıdalarda bulunan doğal koruyucular. Gıda Teknolojileri Elektronik Dergisi, 2: 27-33.
- Dar, P., Farman, M., Dar, A., Khan, Z., Munir, R., Rasheed, A., Waqas, U. (2017). Evaluation of antioxidant potential and comparative analysis of antimicrobial activity of various extracts of Cucurbita pepo L. leaves. J Agric Sci Food Technol, 3: 103-109.
- Deng, Y., Yang, G., Yue, J., Qian, B., Liu, Z., Wang, D., Zhong, Y., Zhao, Y. (2014). Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control, 38: 184-191 doi: 10.1016/j.foodcont.2013.10.023.
- Dulf, F.V., Vodnar, D.C., Dulf, E.H., Pintea, A. (2017). Phenolic compounds, flavonoids, lipids and antioxidant potential of apricot (Prunus armeniaca L.) pomace fermented by two filamentous fungal strains in solid state system. Chem Cent J, 11(1): 1-10, doi: 10.1186/s13065-017-0323-z.
- El-Sayed, M.M., Hashash, M.M., Abdel-Hady, A.A., Abdel-Hady, H., Abdel-Lateef, E.E., Morsi, E.A. (2017). Total phenolic and flavonoid contents and antioxidant activity of Lantana camara and Cucurbita pepo (Squash) extracts as well as GC-MS analysis of Lantana camara essential oils. World J Pharm Res, 6(1): 137-153.
- Ghaffar, F., Kainat, B., Shah, H., Akram, M. (2018). Nutritional, physico-chemical, antimicrobial and antioxidant screening of seed and seed oil of Cucurbita pepo grown in Kpk, Pakistan. FUUAST Journal of Biology, 8(1): 41-48.
- Gohari, A.A., Farhoosh, R., Haddad, K.M. (2011). Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo subsp. pepo var. Styriaka) grown in Iran. J Agric Sci Technol, 13: 1053-1063.
- Gomaa, E.Z. (2013). In vitro antioxidant, antimicrobial, and antitumor activities of bitter almond and sweet apricot (Prunus armeniaca L.) kernels. Food Sci Biotechnol, 22(2): 455-463, doi: 10.1007/s10068-013-0101-1.
- Gupta, A., Sharma, P.C., Tilakratne, B.M.K.S., Verma, A.K. (2012). Studies on physico-chemical characteristics and fatty acid composition of wild apricot (Prunus armeniaca Linn.) kernel oil. Indian Journal of Natural Products and Resources, 3(3): 366-370.
- Gyawali, R., Ibrahim, S.A. (2014). Natural products as antimicrobial agents. Food Control, 46: 412-429, doi: 10.1016/j.foodcont.2014.05.047.
- Hashash, M.M., El-Sayed, M.M., Abdel-Hady, A.A., Hady, H.A., Morsi, E.A. (2017). Nutritional potential, mineral composition, and antioxidant activity Squash (Cucurbıta pepo L.) fruits grown in Egypt. Inflammation, 9(10): 11-12.
- Juhaimi, F.A., Özcan, M.M., Ghafoor, K., Babiker, E.E. (2018). The effect of microwave roasting on bioactive compounds, antioxidant activity and fatty acid composition of apricot kernel and oils. Food Chem, 243: 414-419, doi: 10.1016/j.foodchem.2017.09.100.
- Karaca, E., Aytaç, S. (2007). Yağ bitkilerinde yağ asitleri kompozisyonu üzerine etki eden faktörler. Anadolu Tarım Bilimleri Dergisi, 22(1): 123-131.
- Kasnak, C., Palamutoğlu, R. (2015). Doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri. Turkish Journal of Agriculture-Food Science and Technology, 3(5): 226-234, doi: 10.24925/turjaf.v3i5.226-234.171.
- Kiralan, M., Özkan, G., Bayrak, A., Ramadan, M.F. (2014). Physicochemical properties and stability of black cumin (Nigella sativa) seed oil as affected by different extraction methods. Ind Crop Prod, 57: 52-58, doi: 10.1016/j.indcrop.2014.03.026.
- Korekar, G., Stobdan, T., Arora, R., Yadav, A., Singh, S.B. (2011). Antioxidant capacity and phenolics content of apricot (Prunus armeniaca L.) kernel as a function of genotype. Plant Foods Hum Nutr, 66(4): 376-383, doi: 10.1007/s11130-011-0246-0.
- Lee, H.H., Ahn, J.H., Kwon, A.R., Lee, E.S., Kwak, J.H., Min, Y.H. (2014). Chemical composition and antimicrobial activity of the essential oil of apricot seed. Phytotherapy Res, 28(12): 1867-1872, doi: 10.1002/ptr.5219.
- Li, X.J., Li, Z.G., Wang, X., Han, J.Y., Zhang, B., Fu, Y.J., Zhao, C.J. (2016). Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect. Food Chem, 212: 403-410, doi: 10.1016/j.foodchem.2016.05.185.
- Manzoor, M., Anwar, F., Ashraf, M., Alkharfy, K.M. (2012). Physico-chemical characteristics of seed oils extracted from different apricot (Prunus armeniaca L.) varieties from Pakistan. Grasas Y Aceites, 63(2): 193-201, doi: 10.3989/gya.095011.
- Meru, G., Fu, Y., Leyva, D., Sarnoski, P., Yagiz, Y. (2018). Phenotypic relationships among oil, protein, fatty acid composition and seed size traits in Cucurbita pepo. Sci Hortic, 233: 47-53, doi: 10.1016/j.scienta.2018.01.030.
- Morittu, V.M., Musco, N., Mastellone, V., Bonesi, M., Britti, D., Infascelli, F., Loizzo, M.R., Tundis, R., Sicari, V., Tudisco, R., Lombardi, P. (2019). In vitro and in vivo studies of Cucurbita pepo L. flowers: chemical profile and bioactivity. Nat Prod Res, 30: 1-5, doi: 10.1080/14786419.2019.1672067.
- Nafis, A., Kasrati, A., Jamali, C. A., Custódio, L., Vitalini, S., Iriti, M., Hassani, L. (2020). A Comparative study of the in vitro antimicrobial and synergistic effect of essential oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with antimicrobial drugs: New approach for health promoting products. Antibiotics, 9(4): 140, doi: 10.3390/antibiotics9040140.
- Naik, D.G., Dandge, C.N., Rupanar, S.V. (2011). Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from Gymnema sylvestre R. Br. leaves. J Essent Oil Res, 23(3): 12-19, doi: 10.1080/10412905.2011.9700451.
- Nourmohammadi, E., SadeghiMahoonak, A., Alami, M., Ghorbani, M. (2017). Amino acid composition and antioxidative properties of hydrolysed pumpkin (Cucurbita pepo L.) oil cake protein. Int J Food Prop, 20(12): 3244-3255, doi: 10.1080/10942912.2017.1283516.
- Preuss, H.G., Echard, B., Enig, M., Brook, I., Elliott, T.B. (2005). Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram-positive and gram-negative bacteria. Mol Cell Biochem, 272(1-2): 29-34, doi: 10.1007/s11010-005-6604-1.
- Proestos, C., Boziaris, I.S., Nychas, G.J.E., Komaitis, M. (2006). Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chem, 95: 664-671, doi: 10.1016/j.foodchem.2005.01.049.
- Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26: 1231-1237, doi: 10.1016/s0891-5849(98)00315-3.
- Sener, B., Orhan, I., Ozcelik, B., Kartal, M., Aslan, S., Ozbilen, G. (2007). Antimicrobial and antiviral activities of two seed oil samples of Cucurbita pepo L. and their fatty acid analysis. Natural Product Communications, 2(4): 395-398.
- Sharma, S., Satpathy, G., Gupta, R.K. (2014). Nutritional, phytochemical, antioxidant and antimicrobial activity of Prunus armenicus. J Pharmacogn Phytochem: 3(3), 23-28.
- Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult, 16: 144-158.
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