DETERMINATION OF PHYSICOCHEMICAL, ANTIOXIDANT, ANTIMICROBIAL AND ANTIQUORUM SENSING PROPERTIES OF CHITOSAN FILMS INCORPORATED WITH ETHYL PYRUVATE FILMS

Öz

The aim of this study is to develop an environmentally friendly packaging material by adding ethyl pyruvate (EP) to the chitosan film (CS) formulation in two different concentrations (1% and 3%). Thus, the moisture, water solubility, apparent density, color, biodegradability, and chemical resistance of the films were determined, and the surface morphology was characterized by SEM and functional groups were characterized by FTIR. Additionally, the antimicrobial and anti-qs activities of the films were determined by the disk diffusion method. The moisture, apparent density, biodegradability, and antioxidant activity of chitosan film were increased by adding EP. It was observed that EP caused porous structure in CS. Films showed antimicrobial effect against Escherichia coli O157:H7, Bacillus cereus, Staphylococcus aureus, Alternaria arborescens, Aspergillus flavus, Penicillium digitatum, Penicillium citrinum and Penicillum expansum. It was determined that all films showed anti-qs activity. Results showed that CS-EP1 film can be used as an alternative food packaging

Anahtar Kelimeler

• Chitosan
• ethyl pyruvate
• SEM
• FT-IR
• antimicrobial
• anti-quorum sensing

KİTOSAN BAZLI ETİL PİRÜVAT FİLMLERİN FİZİKOKİMYASAL, ANTİOKSİDAN, ANTİMİKROBİYAL VE ANTİ-QUORUM SENSİNG ÖZELLİKLERİNİN BELİRLENMESİ

Öz

Bu çalışmada kitosan film formülasyonuna iki farklı (%1 ve %3) konsantrasyonda etil pirüvat ilave edilerek, çevre dostu bir ambalaj materyali geliştirilmesi amaçlanmıştır. Bu kapsamda hazırlanan film örneklerinin nem, suda çözünürlük, görünür yoğunluk, renk, biyobozunurluk, kimyasallara karşı direnç özellikleri belirlenerek, filmlerin yüzey morfolojisi SEM, fonksiyonel grupları ise FTIR ile karakterize edilmiştir. Ayrıca film örneklerinin antimikrobiyal ve anti-quorum sensing aktivitesi disk difüzyon yöntemi ile belirlenmiştir. Sonuç olarak formülasyona etil pirüvat eklenmesiyle kitosan filmin nem içeriği, görünür yoğunluğu, biyobozunurluğu ve antioksidan aktivitesi artmıştır. SEM görüntüleri incelendiğinde ise etil pirüvatın, kitosan filmlerde gözenekli yapı oluşumuna neden olduğu gözlemlenmiştir. Film örnekleri Escherichia coli O157:H7, Bacillus cereus, Staphyloccocus aureus, Alternaria arborescens, Aspergillus flavus, Penicillium digitatum, Penicillium citrinum ve Penicillum expansum suşlarına karşı antimikrobiyal etki göstermiştir. Buna ek olarak tüm film örneklerinin anti-quorum sensing aktivite gösterdiği tespit edilmiştir. Elde edilen verilere göre özellikle CS-EP1 filminin, doğa dostu alternatif gıda ambalajı olarak kullanılabileceği belirlenmiştir

Anahtar Kelimeler

• Kitosan
• etil pirüvat
• SEM
• FT-IR
• antimikrobiyal
• anti-quorum sensing

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