Kekik, Nane, Uçkun, Üzüm Çekirdeği ve Yeşil Çay Çöpü Lifinde Optimum Etanolik Ekstraksiyon Şartlarının ve Fenolik Profillerinin Belirlenmesi

Öz

Bu çalışmanın amacı beş bitkinin antioksidan özelliklerini ve farklı etanol oranlarının ektraksiyona etkisini belirlemektir. Kekik, nane, uçkun, üzüm çekirdeği ve yeşil çay çöpü lifinin toplam fenolik madde içeriği (TPC) ve ABTS ve DPPH radikal giderme aktivitesi testleri kullanılarak antioksidan aktiviteleri ölçülmüştür. Bireysel fenolik maddeler ters faz yüksek performanslı sıvı kromotografisi (HPLC) kullanılarak analiz edildi. TPC bitki türü ve çözücü etanol oranına bağlı olarak 2.00±0.27 ile 172.68±0.19 mg GAE g-1 dw arasında değişti. Etanol oranının etkisi farklı bitkiler için değişiklik gösterdi. En yüksek antioksidan aktiviteye sahip ekstraktların HPLC analizleri en yüksek fenolik bileşen konsantrasyonu yeşil çay çöpü lifinde (699.89 mg 100 g-1 dw), en düşük ise nanede (173.67 g 100 g-1 dw) bulundu. Toplam fenolik madde içeriği ve antioksidan aktivite arasında daha önceki çalışmalarda da gösterildiği gibi kayda değer bir korelasyon belirlendi.

Anahtar Kelimeler

• antioksidan
• antioksidan aktivite
• fenolik bileşenler
• etanolik ekstraksiyon

Determination of Optimum Ethanolic Extraction Conditions and Phenolic Profiles of Thyme, Mint, Uckun, Grape Seeds and Green Tea Waste Fiber

Abstract

The objective of this study was to investigate the effect of different ethanol ratios in extraction solvent as well as the antioxidant properties of five plants. Thyme, mint, uckun, grape seeds and green tea waste fiber was analyzed to determine total phenolic content (TPC) and antioxidant activity by ABTS and DPPH radical scavenging activity assays. Individual phenolic components were analyzed with reverse phase high performance liquid chromatography (HPLC). TPC varied significantly from 2.00±0.27 to 172.68±0.19 mg GAE g-1 dw depending on the plant type and ethanol ratio of the solvent. The effect of ethanol ratio also varied among different plants. HPLC analysis was performed for the extracts showing highest antioxidant activity, and green tea waste fiber (699.89 mg 100 g-1 dw) had the highest concentration of phenolic compounds overall, while mint (173.67 mg 100 g-1 dw) had the lowest amount. Correlations between TPC and antioxidant activity was significant which is comparable to the previous report.

Keywords

• Antioxidants
• Antioxidant activity
• Phenolic compounds
• Ethanolic extraction

Kaynakça

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