Effects of shoot tip colchicine applications on some grape cultivars

Year 2021, Volume: 5 Issue: 1, 78 - 84, 31.03.2021

Zeki Kara , Kevser Yazar

https://doi.org/10.31015/jaefs.2021.1.11

Cited By: 1

Abstract

Polyploidization can provide changes in vital features such as growth, development, environmental stress tolerance in plants. Colchicine is one of the most commonly used chemicals as a polyploidization agent. In this study, 2-year-old ‘Ekşi Kara’, ‘Gök Üzüm’ and ‘Trakya İlkeren’ (2x, Vitis vinifera L.) saplings grown on their own roots were used. When the enforced shoots reached about 15 cm length, colchicine applied (0, 2.5 g L-1, 5 g L-1, 7.5 g L-1) 24 and 48 hours to the lateral shoot tips. The effects of treatments were evaluated by shoot tip viability, stoma size and density, chloroplast counts, and flow cytometry (FC) analysis, and ‘Kyoho’ (4x) used as control. The maximum stomatal variations were determined in Ekşi Kara cultivar at 2.5 g L-1 24-h application. Based on morphological differences, FC analysis was performed only in ‘Ekşi Kara’ but, there was no genomic duplication. Since the morphological differences were not sufficient in the diagnosis of polyploid in grape cultivars, FC analysis should be performed to achieve confirmed results. 

Keywords

Grapevine , Cultivar development , Breeding , Chemical mutagen , Autotetraploidy

Supporting Institution

Selçuk University

Project Number

Project Number: 15101013

References

• Acanda, Y., Martínez, Ó., González, M., Prado, M., Rey, M. (2015). Highly efficient in vitro tetraploid plant production via colchicine treatment using embryogenic suspension cultures in grapevine (Vitis vinifera cv. Mencía). Plant Cell, Tissue and Organ Culture, 123(3), 547-555. Doi: https://doi.org/10.1007/s11240-015-0859-3
• Acanda, Y., Prado, M., González, M., Rey, M. (2013). Somatic embryogenesis from stamen filaments in grapevine (Vitis vinifera L. cv. Mencía): changes in ploidy level and nuclear DNA content. In Vitro Cellular & Developmental Biology-Plant, 49(3), 276-284. Doi: https://doi.org/10.1007/s11627-013-9499-7
• Allum, J., Bringloe, D., Roberts, A. (2007). Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time. Plant Cell Reports, 26(11), 1977-1984. Doi: https://doi.org/10.1007/s00299-007-0411-y
• Chen, J., Tang, X., Ma, X., Zhao, Q., Liang, F. (2009). Identification of the ploidy structure of bud sport of Red Globe grape cultivar. Journal of Fruit Science, 26(5), 619-622, Retrieved from https://www.cabdirect.org/cabdirect/abstract/20093358995
• Değirmenci Karataş,D. ,Kunter,B., Coppola,G. And Velasco,R. (2010). Determination of polymorphism using SSCP markers in gamma –irradiated (Co60) grape varieties (Vitis vinifera L.). Genetics and Molecular Research, 9 (4),2357-2363. Doi: https://doi.org/10.4238/vol9-4gmr864
• Dhooghe, E., Van Laere, K., Eeckhaut, T., Leus, L.,Van Huylenbroeck, J. (2011). Mitotic chromosome doubling of plant tissues in vitro. Plant Cell, Tissue and Organ Culture, 104(3), 359-373. Doi: https://doi.org/10.1007/s11240-010-9786-5
• Gülcü, M., Ghafoor, K., Al-Juhaimi, F., Özcan, M. M., Uslu, N., Babiker, E. E., Mohamed Ahmed I.A., Azmi, I. U. (2020). Effect of grape (Vitis vinifera L.) varieties and harvest periods on bioactive compounds, antioxidant activity, phenolic composition, mineral contents, and fatty acid compositions of Vitis leave and oils. Journal of Food Processing and Preservation, 44(11), 1-17. Doi: https://doi.org/10.1111/jfpp.14890
• He, M., Gao, W., Gao, Y., Liu, Y., Yang, X., Jiao, H., Zhou, Y. (2016). Polyploidy induced by colchicine in Dendranthema indicum var. aromaticum, a scented chrysanthemum. European Journal of Horticulture Science, 81(4), 219-226. Doi: https://doi.org/10.17660/eJHS.2016/81.4.5
• Kara, Z., Sabır, A., Yazar, K., Doğan, O., Khaleel, A. (2017a). Fertilization Biology Of Ancient Grape ‘Ekşi Kara’(Vitis vinifera L.). Selcuk Journal of Agriculture and Food Sciences, 31(2), 92-97. Doi: http://dx.doi.org/10.15316/SJAFS.2017.25
• Kara, Z., Sabır, A., Yazar, K., Doğan, O., Omar, A. (2017b). Fruitfulness of Ancient Grapevine Variety ‘Ekşi Kara’(Vitis vinifera L.). Selcuk Journal of Agriculture and Food Sciences, 31(3), 62-68. Doi: http://dx.doi.org/10.15316/SJAFS.2017.36
• Kara, Z., Sabır, A., Yazar, K., Doğan, O., Şit, M. (2018). Effects of colchicine treatments on some grape rootstock and grape varieties at cotyledon stage. Selcuk Journal of Agriculture and Food Sciences, 32(3), 424-429. Doi: http://dx.doi.org/10.15316/SJAFS.2018.117
• Kuliev, V. (2011). Induced autotetraploid grape mutants. Cytology and Genetics, 45(3), 163. Doi: https://doi.org/10.3103/S0095452711030054
• Köse, B., Ateş, S. (2017). Seasonal changes of shoot carbohydrates and growth characteristics of ‘Trakya İlkeren’grape variety (Vitis vinifera L.). Erwerbs-Obstbau, 59(1), 61-70. Doi: https://doi.org/10.1007/s10341-016-0298-2
• Ma, X., Dong, Z., Zhao, Q., Li, X., Tan, W., Tang, X., Chen, J. (2014). A series of Polyploid grape cultivars and their structural identification of ploidy character. Paper presented at the XI International Conference on Grapevine Breeding and Genetics 1082. Doi: https://doi.org/10.17660/ActaHortic.2015.1082.7
• Moghbel, N., Borujeni, M., Bernard, F. (2015). Colchicine effect on the DNA content and stomata size of Glycyrrhiza glabra var. glandulifera and Carthamus tinctorius L. cultured in vitro. Journal of Genetic Engineering and Biotechnology, 13(1), 1-6. Doi: https://doi.org/10.1016/j.jgeb.2015.02.002
• Motosugi, H., Okudo, K., Kataoka, D., Naruo, T. (2002). Comparison of growth characteristics between diploid and colchicine-induced tetraploid grape rootstocks. Journal of the Japanese Society for Horticultural Science, 71(3), 335-341. Doi: https://doi.org/10.2503/jjshs.71.335
• Notsuka, K., Tsuru, T., Shiraishi, M. (2000). Induced polyploid grapes via in vitro chromosome doubling. Journal of the Japanese Society for Horticultural Science, 69(5), 543-551. Doi: https://doi.org/10.2503/jjshs.69.543
• OIV. (2019). 2019 World Vitiviniculture Situation. (20.07.2020) Retrieved from http://www.oiv.int/en/oiv-life/oiv-2019-report-on-the-world-vitivinicultural-situation
• Olmo, H. (1937). Chromosome numbers in the European grape (Vitis vinifera). Cytologia(1), 606-613. Doi: https://doi.org/10.1508/cytologia.FujiiJubilaei.606
• Park, K., Yun, H., Seo, H., Jeong, S., Chung, K., Jun, J., Kang, S. (2004). Breeding of a Black Table Grape Cultivar ‘Heukgoosul’ (Vitis sp.) with Large Berries and High Quality. Korean Journal of Horticultural Science and Technology, 22(4), 462-466. Retrieved from https://www.koreascience.or.kr/article/JAKO200410103435190.page
• Pazuki, A., Aflaki, F., Gürel, E., Ergül, A., Gürel, S. (2018). Gynogenesis induction in sugar beet (Beta vulgaris) improved by 6-benzylaminopurine (BAP) and synergized with cold pretreatment. Sugar Tech, 20(1), 69-77. Doi: https://doi.org/10.1007/s12355-017-0522-x
• Planchais, S., Glab, N., Inzé, D., Bergounioux, C. (2000). Chemical inhibitors: a tool for plant cell cycle studies. Febs Letters, 476(1-2), 78-83. Doi: https://doi.org/10.1016/S0014-5793(00)01675-6
• Sattler, M., Carvalho, C., Clarindo, W. (2016). The polyploidy and its key role in plant breeding. Planta, 243(2), 281-296. Doi: https://doi.org/10.1007/s00425-015-2450-x
• Sekiguchi, F., Yamakawa, K.,Yamaguchi, H. (1971). Radiation damage in shoot apical meristems of Antirrhinum majus and somatic mutations in regenerated buds. Radiation Botany, 11(2), 157-169. Doi: https://doi.org/10.1016/S0033-7560(71)90693-4
• Tuna, M. (2014). Flow sitometri ve tarımsal araştırmalarda kullanımı çalıştayı. Namık Kemal Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü. Tekirdağ. (in Turkish).
• Waite, H., Morton, L. (2007). Hot water treatment, trunk diseases and other critical factors in the production of high-quality grapevine planting material. Phytopathologia Mediterranea, 46(1), 5-17. Retrieved from https://www.jstor.org/stable/pdf/26463266.pdf
• Xie, X., Agüero, C., Wang, Y., Walker, M. (2015). In vitro induction of tetraploids in Vitis x Muscadinia hybrids. Plant cell, tissue and organ culture, 122(3), 675-683. Doi: https://doi.org/10.1007/s11240-015-0801-8
• Yamada, M., Sato, A. (2016). Advances in table grape breeding in Japan. Breeding science, 66(1), 34-45. Doi: https://doi.org/10.1270/jsbbs.66.34
• Yang, X., Cao, Z., An, L., Wang, Y., Fang, X. (2006). In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.). Euphytica, 152(2), 217-224. Doi: https://doi.org/10.1007/s10681-006-9203-7
• Yuan, S., Liu, Y.M., Fang, Z.Y., Yang, L.M., Zhuang, M., Zhang, Y.Y., Sun, P.T. (2009). Study on the relationship between the ploidy level of microspore-derived plants and the number of chloroplast in stomatal guard cells in Brassica oleracea. Agricultural Sciences in China, 8(8), 939-946. Doi: https://doi.org/10.1016/S1671-2927(08)60298-9
• Yue, Y., Zhu, Y., Fan, X., Hou, X., Zhao, C., Zhang, S., Wu, J. (2017). Generation of octoploid switchgrass in three cultivars by colchicine treatment. Industrial Crops and Products, 107, 20-21. Doi: https://doi.org/10.1016/j.indcrop.2017.05.021