Gastroprotective Effects of Jujuba (Ziziphus jujuba) Fruit Extract Against the Ethanol-Related Gastric Ulcer in Rats

Öz

Abstract Objective: Gastric ulcer is a global health problem. Alcohol consumption and nonsteroidal anti-inflammatory drugs may lead to gastric ulcers. Here, we examined the possible gastroprotective effects of Ziziphus jujuba (Zj) fruit extract on a rat model of ethanol-induced gastric ulcer. Material and Methods: Chemical properties of Zj were specified using performance liquid chromatography coupled to mass spectrometry. The animals were administered Zj extract (4-8 ml/kg) before administering absolute ethanol. Oxidative stress and proinflammatory markers, immunoexpression of NF-ĸB and caspase-3 were measured to evaluate Zj’s effects. Results: Zj fruit extract significantly reduced values of cytokines such as TNF-α, IL-1β, and IL-6 and the expressions of NF-ĸB and caspase-3 in the immunohistologic analysis. We found out ulcerative foci in the ulcer group in the histopathological analysis, while we didn’t observe ulcerative foci in the treatment groups. Conclusion: This experimental study showed that Zj fruit ameliorated the ethanol-induced gastric ulcer model in rats.

Anahtar Kelimeler

• Ethanol
• gastric ulcer
• proinflammatory cytokines
• caspase-3
• Ziziphus jujuba

Jujuba (Ziziphus jujuba) Meyve Ekstraktının Ratlarda Etanol ile Oluşturulan Mide Ülzerine Karşı Gastroprotektif Etkileri

Öz

Öz Amaç: Gastrik ülser global bir sağlık sorunudur. Alkol tüketimi ve nonsteroid anti-inflamatuar ilaç kullanımı mide ülserine yol açabilmektedir. Bu çalışmada, Ziziphus jujuba (Zj) meyve ekstraktının ratlarda oluşturdurğumuz etanol kaynaklı mide ülseri modeli üzerindeki olası gastroprotektif etkilerini inceledik. Gereç ve Yöntem: Zj'nin kimyasal özellikleri kütle spektrometresi ile birleştirilmiş performans sıvı kromatografisi kullanılarak belirlendi. Hayvanlara saf etanol verilmeden önce Zj ekstresi (4-8 ml/kg) uygulandı. Zj'nin etkilerini değerlendirmek için oksidatif stres ve proinflamatuar belirteçler, NF-ĸB ve kaspaz-3'ün immünoekspresyonu ölçüldü. Bulgular: Zj meyve ektraktı, immünohistolojik analizde TNF-α, IL-1β ve IL-6 gibi sitokinlerin değerlerini ve NF-ĸB ve kaspaz-3 ekspresyonlarını önemli ölçüde azaltmıştır. Histopatolojik analizde ülser grubunda ülseratif odaklar tespit edilirken, tedavi gruplarında ülseratif odaklar gözlenmedi. Sonuç: Bu deneysel çalışma, Zj meyvesinin sıçanlarda etanol ile indüklenen gastrik ülser modelini iyileştirdiğini göstermiştir.

Anahtar Kelimeler

• Etanol
• gastrik ülser
• proinflamatuar sitokinler
• kaspaz-3
• Ziziphus jujuba

Kaynakça

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