Obtaining of Watermelon Hybrids Superior in term of Some Bioactive Properties

Öz

In this study, 30 watermelon lines which were selected as a result of the data obtained from the “Characterization of Post-Harvest Bioactive Properties of Watermelon Gene Pure Lines in Advanced Breeding Programs” project were hybridized and hybrid seeds were obtained in 2016. These hybrid seeds were planted in an open field in 2017 and 2018 and harvested in July. In 2017, the values of L, a, b, C *, titratable acidity and pH values were found to be statistically insignificant while the value of ho was found to be significant. In 2018, L, a, b, C *, ho, and pH values were found to be significant. Total carotenoid, lycopene and β-carotene values were found to be important in 2017, whereas in 2018, only β-carotene values were found to be significant. Ascorbic acid, total phenol, antioxidant activity, citrullin, pectin methylesterase, chitinase, fructose, glucose, sucrose and total soluble solid values of hybrids in 2017 and 2018 were found to be significant in both years.

Anahtar Kelimeler

• Watermelon
• bioactive characteristics
• hybrid
• lycopene
• carotenoids
• antioxidants

Proje Numarası

TAGEM/BBAD/16/A09/P04/04

Bazı Biyoaktif Özellikler Bakımından Üstün Karpuz Hibritlerin Elde Edilmesi

Öz

Bu çalışmada, “İleri Islah Programlarında Değerlendirmek Üzere Karpuz Gen Havuzundaki Elit Saf Hatların Hasat Sonrası Biyoaktif Özelliklerinin Karakterizasyonu” projesinden elde edilen veriler sonucunda seçilen 30 adet karpuz hattı 2016 yılında melezlenmiş ve hibrit tohumları çıkarılmıştır. Bu hibrit tohumlar 2017 ve 2018 yıllarında açık araziye dikilmiş ve temmuz ayında hasatları yapılmıştır. 2017 yılında çeşitler arasında L, a, b, C*, titre edilebilir asitlik ve pH değerleri açısından farklar istatistiki olarak önemsiz bulunurken, ho değeri önemli bulunmuştur. 2018 yılında ise L, a, b, C*, ho, ve pH değerleri önemli bulunmuştur. Toplam karotenoid, likopen ve β-karoten değerleri açısından 2017 yılında hepsi önemli bulunurken, 2018 yılında ise sadece β-karoten değerleri açısından önemli bulunmuştur. Hibritlerin 2017 ve 2018 yıllarındaki askorbik asit, toplam fenol, antioksidant aktivite, citrullin, pektin metilesteraz, kitinaz, fruktoz, glikoz, sakkaroz ve suda çözünebilir kuru madde değerleri açısından her iki yılda da önemli bulunmuştur.

Anahtar Kelimeler

• Karpuz
• biyoaktif özellikler
• hibrit
• likopen
• karetenoid
• antioksidant

Destekleyen Kurum

Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü

Proje Numarası

TAGEM/BBAD/16/A09/P04/04

Teşekkür

Bu çalışma, Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü tarafından TAGEM/BBAD/16/A09/P04/04 proje numarası ile desteklenmiştir.

Kaynakça

1. Abushita AA, Daood HG, Biacs PA, 2000. Change in Carotenoids and Antioxidant Vitamins in Tomato as a Function of Varietal and Technological Factors. Journal of Agricultural and Food Chemistry, 48: 2075-2081.
2. Bartolome AP, Ruperez P, Fuster C, 1995. Pineapple Fruit: Morphological Characteristics, Chemical Composition And Sensory Analysis Of Red Spanish And Smooth Cayenne Cultivars. Food Chemistry 53. 75-79.
3. Bramley PM, 2000. Is lycopene beneficial to human health? Phytochemistry, 54(3): 233-236
4. Cemeroğlu B, 2010. Gıda Analizleri.Gıda Teknolojisi Derneği yayınları No:34 No: 02-2. Ankara, 18s.
5. Dumas Y, Dadomo M, Lucca GD, Grolier P, Di Lucca G, 2003. Effects of Environmental Factors and Agricultural Techniques on Antioxidant Content of Tomatoes. Journal of the Science of Food and Agriculture 83: 369–382.
6. Fernandez-Caballero C, Romero I, Goni O, Escrıbano MI, Merodio C, Sanchez-Ballesta MT, 2009. Characterization of an Antifungal and Cryoprotective Class I Chitinase from Table Grape Berries (Vitis vinifera Cv. Cardinal). Journal of Agricultural and Food Chemistry, 57 (19): 8893-8900.
7. Fish WW, Perkins-Veazie P, Collins JK, 2002. A quantitative assay for lycopene that utilizes reduced volumes of organic solvents. Journal of Food Composition and Analysis, 15(3): 309-317
8. Gil MI, Aguayo E, Kader AA, 2006. Quality Changes and Nutrient Retention in Fresh-Cut Versus whole Fruits During Storage. Journal of Agricultural and Food Chemistry, 54:4284–4296.

9. Giovannucci E, 1999. Tomatoes, Tomato-Based Products, Lycopene, And Cancer: Review of the Epidemiologic Literature. Journal of the National Cancer Institute, 91: 317–331.
10. Güçdemir İH, 2006. Türkiye Gübre ve Gübreleme Rehberi. Tarım ve Köyişleri Bakanlığı, Tarımsal Araştırmalar Genel Müdürlüğü, Toprak ve Gübre Araştırma Enstitüsü Müdürlüğü yayınları, Genel Yayın Numarası: 231, Teknik Yayın Numarası: T.69. Ankara.
11. ITC, 2016. Uluslararası Ticaret Merkezi Web Sayfası. http://www.intracen.org/
12. Jayaprakasha GK, Chidambara Murthy KN, Patil BS, 2011. Rapid HPLC-UV Method for Quantification of L-Citrulline in Watermelon and its Potential Role on Smooth Muscle Relaxation Markers. Food Chemistry, 127: 240–248.
13. Karaca F, Yetişir H, Solmaz I, Çandır E, Kurt Ş, Sarı N, Güler Z, 2012. Rootstock potential of Turkish Lagenaria siceraria Germplasm for Watermelon: Plant Growth, Yield And Quality. Turkish Journal of Agriculture and Forestry, 36: 167-177.
14. Karaman MR, 2012. Bitki Besleme. 1066 S. Ankara.
15. Klimczak I, Ma"ecka M, Szlachta M, Gliszczynska-Swig"o A, 2007.Effect of Storage on the Content of Polyphenols, Vitamin C and the Antioxidant Activity of Orange Juices. Journal of Food Composition and Analysis, 20: 313–322.
16. Lee HS, Castle WS, Coates GA, 2001. High-Performance Liquid Chromatography for the Characterization of Carotenoids in the New Sweet Orange (Earlygold) Grown in Florida, USA. Journal of Chromatography A, 913 (2001): 371–377.
17. Lee HS, Coates GA,1999. Food Chemistry,65:165-168
18. Lenucci MS, Caccioppola A, Durante M, Serrone L, De Caroli M, Piro G, Dalessandro G, 2009. Carotenoid Content During Tomato (Solanum lycopersicum L.) Fruit Ripening in Traditional and High-Pigment Cultivars. Italian Journal of Food Science, 4 (21): 461–472.
19. Leong LP, Shui G, 2002. An İnvestigation of Antioxidant Capacity of Fruits in Singapore Markets. Food Chemistry, 76:69–75.
20. Leskovar DI, Bang HJ, Crosby K, Maness N, Franco JA, Perkins-Veazie P, 2004. Lycopene, Carbohydrates, Ascorbic Acid, and Yield Components of Diploid and Triploid Watermelon Cultivars are Affected by Deficit İrrigation. Journal of Horticultural Science and Biotechnology, 79 (3): 75–81.
21. Mcguire RG, 1992. Reporting of Objective Color Measurements. HortScience, 27 (12): 1254-1255.
22. Merodio C, Munoz MT, Del Cura B, Buitrago D, Escribano MI, 1998. Effect of High CO2 Level on the Titres of C-Aminobutyric Acid, Total Polyamines and Some Pathogenesis-Related Proteins in Cherimoya Fruit Stored at Low Temperature. Journal of Experimental Botany, 49(325):1339-1347.
23. Muzzarelli RAA, 1977. Chitin. Pergamon Pres, 163.
24. Olives-Barba AI, Hurtado MC, Sanchez-Mata MC, Ruiz VF, Saenz-De Tejado ML, 2006. Application of UV-Vis Detection HPLC Method for a Rapid Determination of Lycopene and B-Carotene in Vegetables. Food Chemistry, 95 (2): 328-336.
25. Oluk AC, Aras V, Ağçam E, Akyildiz A, Sari N, 2017. Some Biochemical Characteristics of Grafted Watermelon. Indian Journal of Horticulture, 74(1): 71-74.
26. Packer L, Rimbach G, Virgili F, 1999. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (pinus maritima) bark, pycnogenol. Free Radical Biology and Medicine, 27(5-6): 704-724.
27. Perkins-Veazie P, 2002. Composition of Orange, Yellow, and Red Fleshed Watermelon. Cucurbitacea, 436–440.
28. Perkins-Veazie P, Collins JK, Clevidence B, 2007. Watermelons and health. Acta Horticulture (ISHS), 731: 121–128.
29. Perkins-Veazie P, Collins JK, Davis AR, Roberts W, 2006. Carotenoid content of 50 watermelon cultivars. Journal of Agricultural and Food Chemistry, 54: 2593–2597
30. Rao AV, 2006. Tomatoes, Lycopene and Human Health. Preventing Chronic Diseases. Caledonian Science Press, Badalona, Spain
31. Rice- Evans CA, Miller NJ,1996. Antioxidant activities of flavonoids as bioactive components of food. Biochemical Society Transactions, 24(3): 790-795.
32. Singleton VL, Rossi JL, 1965. Colorimetry of Total Phenolics with Phosphomolybdic Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16:144-158.
33. Tadmor Y, King S, Levi A, Davis A, Meir A, Wasserman B, Hirchberg J, Lewinsohn E, 2005. Comparative Fruit Colouration in Watermelon and Tomato. Food Research International, 8-9: 837-841.
34. Tarazona-Díaz MP, Viegas J, Moldao-Martins M, Aguayo E, 2011. Bioactive compounds from flesh and by-product of fresh-cut watermelon cultivars. Journal of the Science of Food and Agriculture, 91(5): 805-812.
35. Tlili I, Hdider C, Lenucci, MS, Riadh I, Jebari H, Dalessandro G, 2011. Bioactive Compounds and Antioxidant Activities of Different Watermelon (Citrullus lanatus (Thunb.) Mansfeld) Cultivars as Affected by Fruit Sampling Area. Journal of Food Composition and Analysis, 24: 307-314
36. Tokgöz H, Gölükçü M, Toker R, Yıldız Turgut D, 2015. Karpuzun (Citrullus Lanatus) Bazı Fiziksel Ve Kimyasal Özellikleri Üzerine Aşılı Fide Kullanımı Ve Hasat Zamanının Etkileri. GIDA.
37. Waterman PG, Mole S, 1994. Why Are Phenolic Compounds so İmportant. In: Waterman, P.G., Mole, S. (Eds.), Analysis of Phenolic Plant Metabolites. Blackwell, Oxford, pp. 61–63.
38. Wen CM, Tseng CS, Cheng CY, Li1 YK, 2002. Purification, Characterization and Cloning of a Chitinase from Bacillus sp. NCTU2. Biotechnology and Applied Biochemistry, 35, 213–219.