Starter Kültürlerin Fermente Balık Sucukların Fizikokimyasal ve Besinsel Özellikleri Üzerine Etkileri
Yıl 2025,
Cilt: 15 Sayı: 2, 865 - 880, 15.06.2025
Fatma Öztürk
,
Hatice Gündüz
,
Fettah Gündüz
Öz
Bu çalışmada, amin negatif starter kültürler olan Lactobacillus sakei subsp. sakei, Staphylococcus xylosus ve Pediococcus pentosaceus'un, olgunlaşma ve depolama süresince balık sucuklarının besin bileşimi, tekstür, renk ve amino asit profili üzerindeki etkileri ayrı ayrı ve birlikte incelenmiştir. Fermantasyon sırasında nem içeriğinde anlamlı farklılıklar gözlenmiş ancak depolama sürecinde fark tespit edilmemiştir (P> 0.05). En yüksek kül içeriği S. xylosus içeren sucuklarda bulunmuş ve bu içerik depolama sonunda artmıştır. Renk ölçümleri, L* ve b* değerlerinde azalma olduğunu, a* değerlerinin ise değişkenlik gösterdiğini ortaya koymuştur. Tekstür analizinde gruplar arasında sertlik açısından fark bulunmamıştır. Amino asit analizinde, fermantasyondan sonra toplam serbest amino asit içeriğinde anlamlı bir artış gözlenmiş ve en yüksek seviyeler karışık kültürlerle aşılanmış sucuklarda kaydedilmiştir (P< 0.05). Özellikle lezzet gelişiminde önemli amino asitlerin P. pentosaceus ve karışık kültürlerde daha yüksek olduğu tespit edilmiştir. Sonuçlar, S. xylosus, P. pentosaceus ve karışık kültürlerin, kalite, stabilite ve lezzet geliştirme açısından balık sucuklarında kullanımı için uygun olduğunu göstermektedir.
Proje Numarası
2021-GAP-SUÜF-0003
Kaynakça
-
Afifah, D.N., Ratna Sari, I., Prastifani, N.T., Fulyani, F., Anjani, G., Widyastuti, N., Hastuti, V. N. (2023). Effect of fermentation time on nutrition content, physical properties, ph, amino acids, fatty acids composition and organoleptics on fermented mackerel sausage (Rastrelliger kanagurta Cuvier) characteristics. International Journal of Food Studies, 12(1), 57–70. https://doi.org/10.7455/ijfs/12.1.2023.a4
-
An, Y., Cai, X., Cong, L., Hu, Y., Liu, R., Xiong, S., Hu, X. (2022). Quality ımprovement of zhayu, a fermented fish product in china: effects of inoculated fermentation with three kinds of lactic acid bacteria. Foods, 11(18). https://doi.org/10.3390/foods11182756
-
Antoine, F.R., Wei, C.I., Littell, R.C., Marshall, M.R. (1999). HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. Journal of Agricultural and Food Chemistry, 47(12), 5100–5107. https://doi.org/10.1021/jf990032
-
AOAC. (1984). Official methods of analysis. Association of official analytical chemist (14th ed). Arlington, Washington DC. Washington DC.
-
Aristoy, M.C., Toldra, F. (1991). Deproteinization techniques for HPLC amino acid analysis in fresh pork muscle and dry-cured ham. Journal of Agricultural and Food Chemistry, 39(10), 1792–1795. https://doi.org/10.1021/jf00010a020
-
Arslan, A., Di̇nçoğlu, A.H., Gönülalan, Z. (2001). Fermented Cyprinus carpio L . sausage. Turkish Journal of Veterinary & Animal Sciences, 25(5), 667–673. https://journals.tubitak.gov.tr/veterinary
-
Bıyıklı, M., Akoğlu, A., Kurhan, Ş., Akoğlu, İ.T. (2020). Effect of different Sous Vide cooking temperature-time combinations on the physicochemical, microbiological, and sensory properties of turkey cutlet. International Journal of Gastronomy and Food Science, 20, 100204. https://doi.org/10.1016/j.ijgfs.2020.100204
-
Bligh, E.G., Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. https://doi.org/10.1139/o59-099
-
Chambers E., Bowers J.R. (1993). Consumer perception of sensory qualities in muscle foods. Food Technology, 47(11), 116–120.
-
Hu, Y., Xia, W., Ge, C. (2007). Effect of mixed starter cultures fermentation on the characteristics of silver carp sausages. World Journal of Microbiology and Biotechnology, 23(7), 1021–1031. https://doi.org/10.1007/s11274-006-9330-2
-
Hu, Y., Xia, W., Ge, C. (2008). Characterization of fermented silver carp sausages inoculated with mixed starter culture. LWT-Food Science and Technology, 41(4), 730–738. https://doi.org/10.1016/j.lwt.2007.04.004
-
Ismail, B.P. (2017). Ash content determination (pp. 117–119). https://doi.org/10.1007/978-3-319-44127-6_11
-
Kamiloğlu, A., Kaban, G., Kaya, | Mükerrem. (2019). Effects of autochthonous Lactobacillus plantarum strains on Listeria monocytogenes in sucuk during ripening. Journal of Food Safety, 39(e12618). https://doi.org/10.1111/jfs.12618
-
Lauková, A. (2011). Using natural and novel antimicrobials to improve the safety and shelf-life stability of processed meat products. In Processed meats (pp. 299-330). Woodhead Publishing.
-
Liu, J., Lin, C., Zhang, W., Yang, Q., Meng, J., He, L., Deng, L., Zeng, X. (2021). Exploring the bacterial community for starters in traditional high-salt fermented Chinese fish (Suanyu). Food Chemistry, 358, 129863. https://doi.org/10.1016/j.foodchem.2021.129863
-
Nie, X., Lin, S., Zhang, Q. (2014a). Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus. Food Chemistry, 145, 840–844. https://doi.org/10.1016/j.foodchem.2013.08.096
-
Nie, X., Zhang, Q., Lin, S. (2014b). Biogenic amine accumulation in silver carp sausage inoculated with Lactobacillus plantarum plus Saccharomyces cerevisiae. Food Chemistry, 153, 432–436. https://doi.org/10.1016/j.foodchem.2013.12.093
-
Obadina, A.O., Akinola, O.J., Shittu, T.A., Bakare, H.A. (2013). Effect of natural fermentation on the chemical and nutritional composition of fermented soymilk nono. Nigerian Food Journal, 31(2), 91–97. https://doi.org/10.1016/S0189-7241(15)30081-3
-
Ormancı, H.B. (2013). Palamut (Sarda sarda) lakerdasının olgunlaşması süresince serbest amino asit ve biyojen amin oluşumunun ürün kalitesine etkileri. Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Çanakkale, Türkiye.
-
Rahman, M.S., Alwaili, H., Guizani, N., Kasapis, S. (2007). Instrumental-sensory evaluation of texture for fish sausage and its storage stability. Fisheries Science, 73(5), 1166–1176. https://doi.org/10.1111/j.1444-2906.2007.01449.x
-
Roldán, M., Antequera, T., Martín, A., Mayoral, A.I., Ruiz, J. (2013). Effect of different temperature–time combinations on physicochemical, microbiological, textural and structural features of sous-vide cooked lamb loins. Meat Science, 93(3), 572–578. https://doi.org/10.1016/j.meatsci.2012.11.014
-
Sadeghi, A., Hakimzadeh, V., Salehi, E., Rashidi, H. (2021). Investigation of the physicochemical, organoleptic and dietary properties of trout fermented sausages through probiotic strains and fat replacer. Food Science and Technology, 15(1). https://doi.org/10.15673/fst.v15i1.1968
-
Tagawa, J., Noma, S., Demura, M., Hayashi, N. (2023). Comparison of reduced-salt fish sauces produced under pressurized carbon dioxide treatment from sardinops melanostictus, trachurus japonicus, konosirus punctatus, odontamblyopus lacepedii, and their mixture. Food and Bioprocess Technology, 16(2), 434–446. https://doi.org/10.1007/s11947-022-02920-2
-
TSE (2002), Türk Standartları Enstitüsü. Türk Sucuğu, TS:1070., Ankara.
-
Xu, Y., Xia, W., Yang, F., Kim, J.M., Nie, X. (2010a). Effect of fermentation temperature on the microbial and physicochemical properties of silver carp sausages inoculated with Pediococcus pentosaceus. Food Chemistry, 118(3), 512–518. https://doi.org/10.1016/j.foodchem.2009.05.008
-
Xu, Y., Xia, W., Yang, F., Nie, X. (2010b). Physical and chemical changes of silver carp sausages during fermentation with Pediococcus pentosaceus. Food Chemistry, 122(3), 633–637. https://doi.org/10.1016/j.foodchem.2010.03.023
-
Yin, L.J., Jiang, S.T. (2001). Pediococcus pentosaceus L and S utilization in fermentation and storage of mackerel sausage. Journal of Food Science, 66(5), 742–746. https://doi.org/10.1111/j.1365-2621.2001.tb04631.x
-
Yorukoglu, T., Dayısoylu, K.S. (2016). Some chemical and functional properties of maras tarhana. Atatürk University Journal of Agricultural Faculty, 47(1), 53–63.
-
Yu, S., Yeang, S.B. (1993). Effect of type of starch on the quality of fishballs.In:Liang, O.B., Buchanan, A. and Fardiaz, D. (Eds.), Development of Food Science and Technology in Southeast Asia, IPB Press, 325-332.
-
Yuan, L., Zhang, Q., Zhao, F., Jin, W., Shi, T., Xiong, Z., Gao, R. (2023). Dynamic changes and correlation analysis of protease activities, texture and flavour compounds during fish fermentation (suanyu) using mixed culture ( Enterococcus rivorum and Enterococcus lactis ). International Journal of Food Science & Technology, 58(5), 2312–2324. https://doi.org/10.1111/ijfs.16360
-
Zaman, M.Z., Abu Bakar, F., Jinap, S., Bakar, J. (2011). Novel starter cultures to inhibit biogenic amines accumulation during fish sauce fermentation. International Journal of Food Microbiology, 145(1), 84–91. https://doi.org/10.1016/j.ijfoodmicro.2010.11.031
-
Zeng, X., Xia, W., Jiang, Q., Yang, F. (2013b). Effect of autochthonous starter cultures on microbiological and physico-chemical characteristics of Suan yu, a traditional Chinese low salt fermented fish. Food Control, 33(2), 344–351. https://doi.org/10.1016/j.foodcont.2013.03.001
-
Zeng, X., Xia, W., Yang, F., Jiang, Q. (2013a). Changes of biogenic amines in Chinese low-salt fermented fish pieces (Suan yu) inoculated with mixed starter cultures. International Journal of Food Science and Technology, 48(4), 685–692. https://doi.org/10.1111/ijfs.12010
-
Zhang, Q., Lin, S., Nie, X. (2013). Reduction of biogenic amine accumulation in silver carp sausage by an amine-negative Lactobacillus plantarum. Food Control, 32(2), 496–500. https://doi.org/10.1016/j.foodcont.2013.01.029
-
Zheng, Y., Zhao, G., Zhao, S., Li, X., Cui, W., Xu, L., ... & Tong, L. (2025). Don’t Judge a Sausage by Its Cover: Effects of Inoculating Three Indigenous Lactic Acid Bacteria on Quality, Moisture Distribution, and Protein Structure in Fermentation. Fermentation, 11(3), 134. https://doi.org/10.3390/fermentation11030134
Effects of Starter Cultures on the Physicochemical and Nutritional Characteristics of Fermented Fish Sausages
Yıl 2025,
Cilt: 15 Sayı: 2, 865 - 880, 15.06.2025
Fatma Öztürk
,
Hatice Gündüz
,
Fettah Gündüz
Öz
This study investigated the effects of amine-negative starter cultures, Lactobacillus sakei subsp. sakei, Staphylococcus xylosus, and Pediococcus pentosaceus, both individually and in combination, on the nutritional composition, texture, color, and amino acid profile of fish sausages during ripening and storage. The moisture content differences were significant during fermentation but not during storage (P> 0.05). Sausages with S. xylosus had the highest ash content, which increased by the end of storage. Color measurements showed declines inrevealed decreases in the L* and b* values, whereas the a* values varied with bacterial addition. Texture analysis revealed no significant differences in hardness between the groups. Amino acid analysis demonstrated a notable increase in total free amino acids postfermentation, particularly in sausages inoculated with mixed cultures (P< 0.05). Significant increases in flavor-related amino acids such as alanine, valine, and leucine were observed in sausages with P. pentosaceus and mixed cultures. The results highlight the potential of S. xylosus, P. pentosaceus, and mixed cultures to improve fish sausage quality, stability, and flavor development during storage.
Etik Beyan
The authors declares that this study complies with Research and Publication Ethics.
Destekleyen Kurum
Izmir Katip Celebi University Scientific Research Project Coordination
Proje Numarası
2021-GAP-SUÜF-0003
Teşekkür
This study was supported by Izmir Katip Celebi University Scientific Research Project Coordination (Project number: 2021-GAP-SUÜF-0003).
Kaynakça
-
Afifah, D.N., Ratna Sari, I., Prastifani, N.T., Fulyani, F., Anjani, G., Widyastuti, N., Hastuti, V. N. (2023). Effect of fermentation time on nutrition content, physical properties, ph, amino acids, fatty acids composition and organoleptics on fermented mackerel sausage (Rastrelliger kanagurta Cuvier) characteristics. International Journal of Food Studies, 12(1), 57–70. https://doi.org/10.7455/ijfs/12.1.2023.a4
-
An, Y., Cai, X., Cong, L., Hu, Y., Liu, R., Xiong, S., Hu, X. (2022). Quality ımprovement of zhayu, a fermented fish product in china: effects of inoculated fermentation with three kinds of lactic acid bacteria. Foods, 11(18). https://doi.org/10.3390/foods11182756
-
Antoine, F.R., Wei, C.I., Littell, R.C., Marshall, M.R. (1999). HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. Journal of Agricultural and Food Chemistry, 47(12), 5100–5107. https://doi.org/10.1021/jf990032
-
AOAC. (1984). Official methods of analysis. Association of official analytical chemist (14th ed). Arlington, Washington DC. Washington DC.
-
Aristoy, M.C., Toldra, F. (1991). Deproteinization techniques for HPLC amino acid analysis in fresh pork muscle and dry-cured ham. Journal of Agricultural and Food Chemistry, 39(10), 1792–1795. https://doi.org/10.1021/jf00010a020
-
Arslan, A., Di̇nçoğlu, A.H., Gönülalan, Z. (2001). Fermented Cyprinus carpio L . sausage. Turkish Journal of Veterinary & Animal Sciences, 25(5), 667–673. https://journals.tubitak.gov.tr/veterinary
-
Bıyıklı, M., Akoğlu, A., Kurhan, Ş., Akoğlu, İ.T. (2020). Effect of different Sous Vide cooking temperature-time combinations on the physicochemical, microbiological, and sensory properties of turkey cutlet. International Journal of Gastronomy and Food Science, 20, 100204. https://doi.org/10.1016/j.ijgfs.2020.100204
-
Bligh, E.G., Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. https://doi.org/10.1139/o59-099
-
Chambers E., Bowers J.R. (1993). Consumer perception of sensory qualities in muscle foods. Food Technology, 47(11), 116–120.
-
Hu, Y., Xia, W., Ge, C. (2007). Effect of mixed starter cultures fermentation on the characteristics of silver carp sausages. World Journal of Microbiology and Biotechnology, 23(7), 1021–1031. https://doi.org/10.1007/s11274-006-9330-2
-
Hu, Y., Xia, W., Ge, C. (2008). Characterization of fermented silver carp sausages inoculated with mixed starter culture. LWT-Food Science and Technology, 41(4), 730–738. https://doi.org/10.1016/j.lwt.2007.04.004
-
Ismail, B.P. (2017). Ash content determination (pp. 117–119). https://doi.org/10.1007/978-3-319-44127-6_11
-
Kamiloğlu, A., Kaban, G., Kaya, | Mükerrem. (2019). Effects of autochthonous Lactobacillus plantarum strains on Listeria monocytogenes in sucuk during ripening. Journal of Food Safety, 39(e12618). https://doi.org/10.1111/jfs.12618
-
Lauková, A. (2011). Using natural and novel antimicrobials to improve the safety and shelf-life stability of processed meat products. In Processed meats (pp. 299-330). Woodhead Publishing.
-
Liu, J., Lin, C., Zhang, W., Yang, Q., Meng, J., He, L., Deng, L., Zeng, X. (2021). Exploring the bacterial community for starters in traditional high-salt fermented Chinese fish (Suanyu). Food Chemistry, 358, 129863. https://doi.org/10.1016/j.foodchem.2021.129863
-
Nie, X., Lin, S., Zhang, Q. (2014a). Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus. Food Chemistry, 145, 840–844. https://doi.org/10.1016/j.foodchem.2013.08.096
-
Nie, X., Zhang, Q., Lin, S. (2014b). Biogenic amine accumulation in silver carp sausage inoculated with Lactobacillus plantarum plus Saccharomyces cerevisiae. Food Chemistry, 153, 432–436. https://doi.org/10.1016/j.foodchem.2013.12.093
-
Obadina, A.O., Akinola, O.J., Shittu, T.A., Bakare, H.A. (2013). Effect of natural fermentation on the chemical and nutritional composition of fermented soymilk nono. Nigerian Food Journal, 31(2), 91–97. https://doi.org/10.1016/S0189-7241(15)30081-3
-
Ormancı, H.B. (2013). Palamut (Sarda sarda) lakerdasının olgunlaşması süresince serbest amino asit ve biyojen amin oluşumunun ürün kalitesine etkileri. Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Çanakkale, Türkiye.
-
Rahman, M.S., Alwaili, H., Guizani, N., Kasapis, S. (2007). Instrumental-sensory evaluation of texture for fish sausage and its storage stability. Fisheries Science, 73(5), 1166–1176. https://doi.org/10.1111/j.1444-2906.2007.01449.x
-
Roldán, M., Antequera, T., Martín, A., Mayoral, A.I., Ruiz, J. (2013). Effect of different temperature–time combinations on physicochemical, microbiological, textural and structural features of sous-vide cooked lamb loins. Meat Science, 93(3), 572–578. https://doi.org/10.1016/j.meatsci.2012.11.014
-
Sadeghi, A., Hakimzadeh, V., Salehi, E., Rashidi, H. (2021). Investigation of the physicochemical, organoleptic and dietary properties of trout fermented sausages through probiotic strains and fat replacer. Food Science and Technology, 15(1). https://doi.org/10.15673/fst.v15i1.1968
-
Tagawa, J., Noma, S., Demura, M., Hayashi, N. (2023). Comparison of reduced-salt fish sauces produced under pressurized carbon dioxide treatment from sardinops melanostictus, trachurus japonicus, konosirus punctatus, odontamblyopus lacepedii, and their mixture. Food and Bioprocess Technology, 16(2), 434–446. https://doi.org/10.1007/s11947-022-02920-2
-
TSE (2002), Türk Standartları Enstitüsü. Türk Sucuğu, TS:1070., Ankara.
-
Xu, Y., Xia, W., Yang, F., Kim, J.M., Nie, X. (2010a). Effect of fermentation temperature on the microbial and physicochemical properties of silver carp sausages inoculated with Pediococcus pentosaceus. Food Chemistry, 118(3), 512–518. https://doi.org/10.1016/j.foodchem.2009.05.008
-
Xu, Y., Xia, W., Yang, F., Nie, X. (2010b). Physical and chemical changes of silver carp sausages during fermentation with Pediococcus pentosaceus. Food Chemistry, 122(3), 633–637. https://doi.org/10.1016/j.foodchem.2010.03.023
-
Yin, L.J., Jiang, S.T. (2001). Pediococcus pentosaceus L and S utilization in fermentation and storage of mackerel sausage. Journal of Food Science, 66(5), 742–746. https://doi.org/10.1111/j.1365-2621.2001.tb04631.x
-
Yorukoglu, T., Dayısoylu, K.S. (2016). Some chemical and functional properties of maras tarhana. Atatürk University Journal of Agricultural Faculty, 47(1), 53–63.
-
Yu, S., Yeang, S.B. (1993). Effect of type of starch on the quality of fishballs.In:Liang, O.B., Buchanan, A. and Fardiaz, D. (Eds.), Development of Food Science and Technology in Southeast Asia, IPB Press, 325-332.
-
Yuan, L., Zhang, Q., Zhao, F., Jin, W., Shi, T., Xiong, Z., Gao, R. (2023). Dynamic changes and correlation analysis of protease activities, texture and flavour compounds during fish fermentation (suanyu) using mixed culture ( Enterococcus rivorum and Enterococcus lactis ). International Journal of Food Science & Technology, 58(5), 2312–2324. https://doi.org/10.1111/ijfs.16360
-
Zaman, M.Z., Abu Bakar, F., Jinap, S., Bakar, J. (2011). Novel starter cultures to inhibit biogenic amines accumulation during fish sauce fermentation. International Journal of Food Microbiology, 145(1), 84–91. https://doi.org/10.1016/j.ijfoodmicro.2010.11.031
-
Zeng, X., Xia, W., Jiang, Q., Yang, F. (2013b). Effect of autochthonous starter cultures on microbiological and physico-chemical characteristics of Suan yu, a traditional Chinese low salt fermented fish. Food Control, 33(2), 344–351. https://doi.org/10.1016/j.foodcont.2013.03.001
-
Zeng, X., Xia, W., Yang, F., Jiang, Q. (2013a). Changes of biogenic amines in Chinese low-salt fermented fish pieces (Suan yu) inoculated with mixed starter cultures. International Journal of Food Science and Technology, 48(4), 685–692. https://doi.org/10.1111/ijfs.12010
-
Zhang, Q., Lin, S., Nie, X. (2013). Reduction of biogenic amine accumulation in silver carp sausage by an amine-negative Lactobacillus plantarum. Food Control, 32(2), 496–500. https://doi.org/10.1016/j.foodcont.2013.01.029
-
Zheng, Y., Zhao, G., Zhao, S., Li, X., Cui, W., Xu, L., ... & Tong, L. (2025). Don’t Judge a Sausage by Its Cover: Effects of Inoculating Three Indigenous Lactic Acid Bacteria on Quality, Moisture Distribution, and Protein Structure in Fermentation. Fermentation, 11(3), 134. https://doi.org/10.3390/fermentation11030134