Effects of Organic Acid Treatments and Modified Atmosphere Packaging on the Presence of Staphylococcus aureus and Escherichia coli O157:H7 and Shelf-life in Meatballs

Öz

This study was carried out to determine the effects of modified atmosphere packaging (ambient air, 80:20/O2:CO2 - MAP-O2, 0.4:30:69.60/CO:CO2:N2 - MAP-CO) combined with organic acid (1% and 2% sodium lactate, 0.5% potassium sorbate, 0.5% sodium citrate and 1% sodium acetate) addition on the presence of Staphylococcus aureus and Escherichia coli O157:H7 in meatballs and the quality changes during cold storage. In this context, meatball samples experimentally contaminated with S. aureus and E. coli O157:H7 strains were treated with the relevant organic acids and then subjected to modified atmosphere packaging process. The samples were evaluated in terms of microbiological, physicochemical and sensory parameters during the 15-day storage period. The addition of 2% sodium lactate provided the highest inhibition on the number of S. aureus and E. coli O157:H7 in meatballs among the organic acids, while the combined CO packaging was the highest inhibition among the treatments. It was determined that off-odour formation was decreased in meatballs added with organic acids and packaged with CO, while red colour stability was achieved in sodium lactate-treated samples, the highest red colour values were observed in meatball samples packaged with CO. In conclusion, the application of organic acid treatment in combination with modified atmosphere packaging inhibits pathogenic microorganisms while prolonging the shelf-life and preserving the sensory properties of meatballs.

Anahtar Kelimeler

• Escherichia coli O157:H7
• Meatballs
• Modified Atmosphere Packaging
• Organic acid
• Shelf-life
• Staphylococcus aureus

Organik Asit Uygulamalarının ve Modifiye Atmosfer Paketlemenin Köftelerde Staphylococcus aureus ve Escherichia coli O157:H7 Varlığı ve Raf Ömrü Üzerine Etkileri

Öz

Bu çalışma, organik asit (%1 ve %2 sodyum laktat, %0,5 potasyum sorbat, %0,5 sodyum sitrat ve %1 sodyum asetat) ilavesi ile kombine modifiye atmosfer paketleme uygulamalarının (ortam havası, 80:20/O2:CO2 - MAP-O2, 0,4:30:69,60/CO:CO2:N2 - MAP-CO) köftelerde Staphylococcus aureus ile Escherichia coli O157:H7 varlığı üzerine etkilerini belirlemek ve soğuk muhafaza süresince kalite değişimlerini ortaya koymak amacıyla gerçekleştirilmiştir. Bu kapsamda, deneysel olarak S. aureus ve E. coli O157:H7 suşları ile kontamine edilen köfte örnekleri ilgili organik asitler ile muamele edildikten sonra modifiye atmosfer paketleme işlemine tabi tutulmuştur. Örnekler 15 günlük muhafaza süresi boyunca mikrobiyolojik, fizikokimyasal ve duyusal parametreler yönünden değerlendirilmiştir. %2 sodyum laktat ilavesi köftelerdeki S. aureus ve E. coli O157:H7 sayısı üzerinde organik asitler içerisinde en yüksek inhibisyonu sağlarken, kombine olarak CO ile paketleme uygulamalar arasında en yüksek inhibisyon kaydedilen işlem olmuştur. Organik asit ilave edilen ve CO ile paketlenen köftelerde kötü koku oluşumunun azaldığı, sodyum laktat ile muamele edilen örneklerde kırmızı renk stabilitesi sağlanırken en yüksek kırmızı renk değerlerinin CO ile paketli köfte örneklerinde gözlendiği belirlenmiştir. Sonuç olarak, organik asit muamelesinin modifiye atmosfer paketlemeyle birlikte uygulanması patojen mikroorganizmalar üzerinde inhibisyon oluştururken, köftelerin raf ömrünün uzamasına ve duyusal özelliklerinin de korunmasına olanak sağlamaktadır.

Anahtar Kelimeler

• Escherichia coli O157:H7
• Köfte
• Modifiye Atmosfer Paketleme
• Organik Asit
• Raf Ömrü
• Staphylococcus aureus

Kaynakça

1. Abu-Ghazaleh, B. M. (2013). Effects of ascorbic acid, citric acid, lactic acid, NaCl, potassium sorbate and Thymus vulgaris extract on Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 7, 7-12. https://doi.org/10.5897/AJMR12.042
2. Amin Zare, M., Razavi Rohani, S. M., Raeisi, M., Javadi Hosseini, S. H., & Hashemi, M. (2014). Antibacterial effects of monolaurin, sorbic acid and potassium sorbate on Staphylococcus aureus and Escherichia coli. Journal of Food Quality and Hazards Control, 1, 52-55.
3. AOAC (2005). Official methods of analysis of the Associa-tion of the Analytical Chemists (18th Ed.) (Washington). Determination of pH. Method 940.23.
4. Beterams, A., Tolksdorf, T., Martin, A., Stingl, K., Bandick, N., & Reich, F. (2023). Change of Campylobacter, Escherichia coli and Salmonella counts in packaged broiler breast meat stored under modified atmosphere and vacuum conditions at 4 and 10ºC based on cultural and molecular biological quantification. Food Control, 145, 109337. https://doi.org/10.1016/j.foodcont.2022.109337
5. Bingol, E. B., Colak, H., Cetin, O., & Hampikyan, H. (2014). Effects of sodium lactate on the shelf life and sensory characteristics of cig köfte - A Turkish traditional raw meatball. Journal of Food Processing and Preservation, 38, 1024-1036. https://doi.org/10.1111/jfpp.12059
6. Bingol, E. B., Colak, H., Cetin, O., Kahraman, T., Hampikyan, H. & Ergun, O. (2012). Effects of high-Oxygen modified atmosphere packaging on the microbiological quality and shelf-life of Tekirdag kofte: A Turkish type meatball. Journal of Animal and Veterinary Advances, 11, 3148-3155. https://doi.org/10.3923/javaa.2012.3148.3155
7. Bingol, E. B., & Ergun, O. (2011). Effects of modified atmosphere packaging (MAP) on the microbiological quality and shelf life of ostrich meat. Meat Science, 88, 774-785. https://doi.org/10.1016/j.meatsci.2011.03.013
8. Byrne, C. M., Bolton, D. J., Sheridan, J. J., Blair, I. S., & McDowell, D. A. (2002). Determination of the effect of sodium lactate on the survival and heat resistance of Escherichia coli O157:H7 in two commercial beef patty formulations. Food Microbiology, 19, 211-219. https://doi.org/10.1006/fmic.2001.0462

9. Coban, H. B. (2020). Organic acids as antimicrobial food agents: Applications and microbial productions. Bioprocess and Biosystems Engineering, 43, 569-591. https://doi.org/10.1007/s00449-019-02256-w
10. Cornforth, D., & Hunt, M. (2008). The American Meat Science Association. Low-oxygen packaging of fresh meat with carbon monoxide. Meat quality, microbiology and safety. White Paper Ser.2.
11. Djenane, D., Beltrán, J. A., Camo, J. & Roncalés, P. (2016). Influence of vacuum at different ageing times and subsequent retail display on shelf life of beef cuts packaged with active film under high O2. Journal of Food Science and Technology, 53, 4244-4257. https://doi.org/10.1007/s13197-016-2419-1
12. Djenane, D., & Roncalés, P. (2018). Carbon Monoxide in Meat and Fish Packaging: Advantages and Limits. Foods, 7, 12. https://doi.org//10.3390/foods7020012
13. Djordjević, J., Bošković, M., Starčević, M., Ivanović, J., Karabasil, N., Dimitrijević, M., Lazić, I. B., & Baltić, M. Ž. (2018). Survival of Salmonella spp. in minced meat packaged under vacuum and modified atmosphere. Brazilian Journal of Microbiology, 49, 607-613. https://doi.org/10.1016/j.bjm.2017.09.009
14. Farber, J. M. (1991). Microbiological aspects of modified-atmosphere packaging technology-a review. Journal of Food Protection, 54, 58-70. https://doi.org/10.4315/0362-028X-54.1.58
15. Hoffman, L. C., Muller, M., & Vermaak, A. (2008). Sensory and preference testing of selected beef muscles infused with a phosphate and lactate blend. Meat Science, 80, 1055-1060. https://doi.org/10.1016/j.meatsci.2008.04.025
16. Hwang, C. A., & Juneja, V. (2011). Effects of salt, sodium pyrophosphate, and sodium lactate on the probability of growth of Escherichia coli O157:H7 in ground beef. Journal of Food Protection, 74, 622-626. https://doi.org/10.4315/0362-028X.JFP-10-325
17. Igwegbe, A. O., Idakwo, P. Y., Yusuf, H. L., Agbara, G. I., Maijalo, A. I. & Abubakar, F. (2019). Effects of sodium citrate and garlic on organoleptic properties, proximate composition, free fatty acid and thiobarbituric acid levels of treated smoke-dried meat stored at ambient temperatures. CPQ Medicine, 5, 1-14.
18. ISO 13299 (2016). Sensory Analysis - Methodology, General Guidance for Establishing a Sensory Profile.
19. ISO 16654 (2001). Microbiology of the food and animal feding stuffs. Horizontal method for the detection of Escherichia coli O157.
20. ISO 18787 (2017). Foodstuffs - Determination of water activity.
21. ISO 6887-1 (2017). Microbiology of the food chain. Preparation of test samples, initial suspension and decimal dilutions for microbiological examination-Part 1: General rules for the preparation of the initial suspension and decimal dilutions.
22. ISO 6888-1 (2021). Microbiology of the food chain. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Method using Baird-Parker agar medium.
23. ISO 8586 (2023). Sensory analysis. General guidelines for the selection, training and monitoring of selected assessors and expert sensory assessors.
24. Jaspal, M. H., Ijaz, M., Anwaar ul Haq, A., Yar, M. K., Asghar, B., Manzoor, A., Badar, I. H., Ullah, S., Islam, Md. S. & Hussain, J. (2021). Effect of oregano essential oil or lactic acid treatments combined with air and modified atmosphere packaging on the quality and storage properties of chicken breast meat. LWT-Food Science and Technology, 146, 111459. https://doi.org/10.1016/j.lwt.2021.11145
25. Kim, Y. H., Hunt, M. C., Mancini, R. A., Seyfert, M., Loughin, T. M., Kropf, D. H. & Smith, J. S. (2006). Mechanism for lactate-color stabilization in injection-enhanced beef. Journal of Agricultural and Food Chemistry, 54, 7856-7862. https://doi.org/10.1021/jf061225h
26. Lee, S., Decker, E. A. & Faustman, C. (2005). The effects of antioxidant combinations on color and lipid oxidation in n-3 oil fortified ground beef patties. Meat Science, 70, 683-689. https://doi.org/10.1016/j.meatsci.2005.02.017
27. Lim, K., & Mustapha, A. (2004). Effects of cetylpyridinium chloride, acidified sodium chlorite, and potassium sorbate on populations of Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on fresh beef. Journal of Food Protection, 67, 310-315. https://doi.org/10.4315/0362-028x-67.2.310
28. Mancini, R. A., Suman, S. P., Konda, M. K., & Ramanathan, R. (2009). Effect of carbon monoxide packaging and lactate enhancement on the color stability of beef steaks stored at 1°C for 9 days. Meat Science, 81, 71-76. https://doi.org/10.1016/j.meatsci.2008.06.021
29. Mancini, R. A., Ramanathan, R., Suman, S. P., Konda, M. K., Joseph, P., Dady, G. A., Naveena, B. M., & López-López, I. (2010). Effects of lactate and modified atmospheric packaging on premature browning in cooked ground beef patties. Meat Science, 85, 339-346. https://doi.org/10.1016/j.meatsci.2010.02.001
30. María, C., Adriana, L. B., Guadalupe, M., & Luis, R. P. J. (2015). Thermal diffusivities and influence of cooking temperature combined with sodium lactate addition on microbiological characteristics of rabbit ham. Advance Journal of Food Science and Technology, 7, 242-249. http://dx.doi.org/10.19026/ajfst.7.1302
31. Meng, X., Wu, D., Zhang, Z., Wang, H., Wu, P., Xu, Z., Gao, Z., Mintah, B. K., & Dabbour, M. (2022). An overview of factors affecting the quality of beef meatballs: Processing and preservation. Food Science & Nutrition, 10, 1961-1974. https://doi.org/10.1002/fsn3.2812
32. Mir, S. A., Masoodi, F. A. (2018). Use of organic acids for preservation and safety of traditional meat products. Journal of Food Safety, 38, 12514.
33. Mortazavi, S.M.H., Kaur, M., Farahnaky, A., Torley, P.J., Osborn, A.M. (2023). The pathogenic and spoilage bacteria associated with red meat and application of different approaches of high CO2 packaging to extend product shelf-life. Critical Reviews in Food Science and Nutrition, 63(12), 1733-1754. https://doi.org/10.1080/10408398.2021.1968336.
34. Ozturk, H. M., Ozturk, H. K., & Koçar, G. (2017). Microbial analysis of meatballs cooled with vacuum and conventional cooling. Journal of Food Science and Technology, 54, 2825-2832. https://doi.org/10.1007/s13197-017-2720-7
35. Pegg, R. B., & Shahidi, F. (2000). Nitrite curing of meat. The N-Nitrosamine Problem and Nitrite Alternatives. Food and Nutrition Press, UK.
36. Ponrajan, A., Harrison, M. A., Segers, J. R., Lowe, B. K., McKeith, R. O., Pringle, T. D., Martino, K. G., Mulligan, J. H., & Stelzleni, A. M. (2011). Effects of sodium citrate plus sodium diacetate and buffered vinegar on Escherichia coli O157:H7 and psychrotrophic bacteria in brine-injected beef. Journal of Food Protection, 74, 359-364. https://doi.org/10.4315/0362-028X.JFP-10-294
37. Quilo, S. A., Pohlman, F. W., Dias-Morse, P. N., Brown, A. H., Crandall, P. G., Baublits, R. T. & Aparicio, J. L. (2009). The impact of single antimicrobial intervention treatment with potassium lactate, sodium metasilicate, peroxyacetic acid, and acidified sodium chlorite on non-inoculated ground beef lipid, instrumental color, and sensory characteristics. Meat Science, 83, 345-350. https://doi.org/10.1016/j.meatsci.2009.05.015
38. Quilo, S. A., Pohlman, F. W., Dias-Morse, P. N., Brown, A. H., Crandall, P. G., & Story, R. P. (2010). Microbial, instrumental color and sensory characteristics of inoculated ground beef produced using potassium lactate, sodium metasilicate or peroxyacetic acid as multiple antimicrobial interventions. Meat Science, 84, 470-476. https://doi.org/10.1016/j.meatsci.2009.09.018
39. Sallam, K. I., & Samejima, K. (2004). Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. Lebensmittel-Wissenschaft & Technologie, 37, 865-871. https://doi.org/10.1016/j.lwt.2004.04.003
40. SPSS 21.00. Statistical Package for the Social Sciences. SPSS Inc., Chicago, IL, USA. Stopforth, J., & Kudron, T. (2020). Sorbic Acid and Sorbates. In: Antimicrobials in Food (4th Ed), CRC Press.
41. Suman, S. P., Mancini, R. A., Joseph, P., Ramanathan, R., Konda, M. K. R., Dady, G., Naveena, B. M., & López-López, I. (2010). Color-stabilizing effect of lactate on ground beef is packaging-dependent. Meat Science, 84, 329-333. https://doi.org/10.1016/j.meatsci.2009.08.051
42. Tenderis, B., Kılıç, B., Yalçın, H., & Şimşek, A. (2020). Impact of sodium lactate, encapsulated or unencapsulated polyphosphates T and their combinations on Salmonella Typhimurium, Escherichia coli O157:H7 and Staphylococcus aureus growth in cooked ground beef. International Journal of Food Microbiology, 321, 108560. https://doi.org/10.1016/j.ijfoodmicro.2020.108560
43. Theron, M. M., & Lues, J. F. (2007). Organic acids and meat preservation: A review. Food Reviews International, 23, 141-158. https://doi.org/10.1080/87559120701224964
44. Uyttendaele, M., Jozwik, E., Tutenel, A., De Zutter, L., Uradzinski, J., Pierard, D., & Debevere, J. (2001). Effect of acid resistance of Escherichia coli O157:H7 on efficacy of buffered lactic acid to decontaminate chilled beef tissue and effect of modified atmosphere packaging on survival of Escherichia coli O157:H7 on red meat. Journal of Food Protection, 64, 1661-1666. https://doi.org/10.4315/0362-028x-64.11.1661
45. Yu, H. H., Song, Y. J., Kim, Y. J., Lee, H. Y., Choi, Y.-S., Lee, N.-K., & Paik, H.-D. (2020). Predictive model of growth kinetics for Staphylococcus aureus in raw beef under various packaging systems. Meat Science, 165, 108108. https://doi.org/10.1016/j.meatsci.2020.108108