Protein ve prebiyotik kaynaklar kullanılarak üretilen içeceklerin fizikokimyasal ve duyusal özelliklerinin belirlenmesi

Yıl 2025, Cilt: 30 Sayı: 2, 487 - 502, 21.08.2025

Emir Ayşe Özer , Neslihan Özbuldu , Beyza Özpalas

https://doi.org/10.37908/mkutbd.1626355

Öz

Bu çalışmanın amacı, her yaş grubundan bireylerin tüketebileceği protein ve prebiyotik içeren içeceklerin fizikokimyasal ve duyusal özelliklerinin belirlenmesidir. Çalışma, Tepki Yüzey Yöntemi (TYY) kullanılarak tasarlanmış ve bağımsız değişken olarak çikolatalı peynir altı suyu protein izolatı (WPI), bezelye protein izolatı (PPI) ve inülin kullanılmıştır. Bu değişkenlerin maksimum ve minimum aralıkları WPI için 5.0-10.0 g/100 mL, PPI için 2.0-6.0 g/100 mL ve inülin için 3.0-7.0 g/100 mL olarak belirlenmiştir. Deneysel tasarım sonucunda, optimum üretim koşulları 9.66 g WPI, 2.00 g PPI ve 5.00 g inülin olarak belirlenmiştir. Bu üretim koşullarında, optimum fizikokimyasal yanıtlar şu şekilde belirlenmiştir: pH değeri 7.02, titrasyon asitliği 0.02, suda çözünür kuru madde içeriği 15.38, L* değeri 46.87, a* değeri 5.79 ve b* değeri 8.54. Duyusal yanıtlar şu şekilde belirlenmiştir: tat değeri 6.16, koku değeri 6.71, ağızda kalan tat değeri 6.50 ve genel kabul edilebilirlik değeri 6.56. Bu koşullar altında optimize edilmiş içeceğin arzu edilebilirlik değerinin %97 olduğu tespit edilmiştir. Bu çalışma ile, duyusal kabul edilebilirliği yüksek yeni bir fonksiyonel içecek formülasyonu geliştirilmiştir.

Anahtar Kelimeler

Fonksiyonel içecekler , Tepki Yüzey Yöntemi , Duyusal değerlendirme , Optimizasyon

Proje Numarası

20.M.041

Determination of physicochemical and sensory properties of beverages produced using protein and prebiotic sources

Öz

The aim of this study was to determine the physicochemical and sensory properties of protein and prebiotic containing beverages that can be consumed by individuals from all age groups. The study was designed by using the Response Surface Method, and chocolate whey protein isolate (WPI), pea protein isolate (PPI) and inulin were used as independent variables. The maximum and minimum ranges of these variables were determined as 5.0-10.0 g/100 mL for WPI, 2.0-6.0 g/100 mL for PPI and 3.0-7.0 g/100 mL for inulin. As a result of the experimental design, the optimum production conditions were determined as 9.66 g WPI, 2.00 g PPI and 5.00 g inulin. Under these production conditions, the optimum physicochemical responses were determined as follows: the pH value was 7.02, the titration acidity was 0.02, the water-soluble dry matter content was 15.38, the L* value was 46.87, the a* value was 5.79, and the b* value was 8.54. Sensory responses were determined as follows: the taste value was 6.16, the odour value was 6.71, the aftertaste value was 6.50, and the overall acceptability value was 6.56. Under these conditions, the desirability value of the optimised beverage was found to be 97%. With this study, a new functional beverage formulation with high sensory acceptability was developed.

Anahtar Kelimeler

Functional beverages , Response Surface Method , Sensory evaluation , Optimisation

Destekleyen Kurum

Hatay Mustafa Kemal University Scientific Research Projects Coordination Office

Proje Numarası

20.M.041

Teşekkür

This study was financially supported by Hatay Mustafa Kemal University Scientific Research Projects Coordination Office (20.M.041).

Kaynakça

• Ahern, N., Arendt, E.K., & Sahin, A.W. (2023). Protein soft drinks: A retail market analysis and selected product characterization. Beverages, 9 (3), 73. https://doi.org/10.3390/beverages9030073
• Akin, Z., & Ozcan, T. (2017). Functional properties of fermented milk produced with plant proteins. LWT - Food Science and Technology, 86, 25-30. https://doi.org/10.1016/j.lwt.2017.07.025
• AOAC. (2000). Official methods of analysis : Association of official analytical chemists (17th ed.). Washington, DC, USA.
• Aribah, S.A., Sanjaya, A.P., Muhammad, D.R.A., & Praseptiangga, D. (2020). Sensorial and physical properties of chocolate beverage prepared using low fat cocoa powder. In AIP Conference Proceedings, 2219 (1), 070007. https://doi.org/10.1063/5.0003435
• Birch, C.S., & Bonwick, G.A. (2019). Ensuring the future of functional foods. International Journal of Food Science & Technology, 54 (5),1467-1485. https://doi.org/10.1111/ijfs.14060
• Boukid, F., Rosell, C.M., & Castellari, M. (2021). Pea protein ingredients: A mainstream ingredient to (re)formulate innovative foods and beverages. Trends in Food Science & Technology, 110, 729-742. https://doi.org/10.1016/j.tifs.2021.02.040
• Bäuerle, L., & Kühn, S. (2022). Development of a protein supplemented fruit smoothie using pea protein isolate as a plant-based protein alternative. Future Foods, 5, 100145. https://doi.org/10.1016/j.fufo.2022.100145
• Carvalho, F., Lahlou, R.A., Pires, P., Salgado, M., & Silva, L.R. (2023). Natural functional beverages as an approach to manage diabetes. International Journal of Molecular Sciences, 24 (23), 16977. https://doi.org/10.3390/ijms242316977
• Çiftçi, M., & Öncül, N. (2022). Some microbiological properties of commercial probiotic drinks (in Turkish). Academic Journal of Agriculture, 11 (1), 165-178. http://dx.doi.org/10.29278/azd.1002242
• Demirbağ, Z., Alan, S., & Öksüztepe, G. (2023). Functional foods and their ımportance in nutrition (in Turkish). Bozok Veterinary Sciences, 4 (2), 54-60. https://doi.org/10.58833/bozokvetsci.1324245
• Doğan, M., Aktar, T., Toker, O.S., & Tatlısu, N.B. (2015). Combination of the simple additive (SAW) approach and mixture design to determine optimum cocoa combination of the hot chocolate beverage. International Journal of Food Properties, 18 (8), 1677-1692. https://doi.org/10.1080/10942912.2014.917662
• Ersus, S., Tekin, İ., Visali, R., Allahverdiyeva, Z., Askerova, İ., Hasanova, A., Kamalov, R., & Aliyeva, D. (2024). Production of functional apple juice enriched with aromatic plant extracts (in Turkish). Journal of Agriculture Faculty of Ege University, 61 (1), 103-111. https://doi.org/10.20289/zfdergi.1433287
• Garay, P.A., Villalva, F.J., Paz, N.F., de Oliveira, E.G., Ibarguren, C., Alcocer, J.C., Curti, C.A., & Ramón, A.N. (2021). Formulation of a protein fortified drink based on goat milk whey for athletes. Small Ruminant Research, 201, 106418. https://doi.org/10.1016/j.smallrumres.2021.106418
• Gayathry, K.S., & John, J.A. (2021). Functional beverages: Special focus on anti-diabetic potential. Journal of Food Processing and Preservation, 45 (11), e15974. https://doi.org/10.1111/jfpp.15974
• Gimhani, K.H.I., & Liyanage, A.L.C.J. (2019). Development and quality evaluation of ready to drink fruit flavoured whey beverage. International Journal of Scientific and Research Publications, 9 (8), 779-783. http://dx.doi.org/10.29322/IJSRP.9.08.2019.p92107
• Hidalgo-Fuentes, B., de Jesús-José, E., Cabrera-Hidalgo, A.J., Sandoval-Castilla, O., Espinosa-Solares, T., González-Reza, R.M., Zambrano-Zaragoza, M.L., Liceaga, A.M., & Aguilar-Toalá, J.E. (2024). Plant-based fermented beverages: Nutritional composition, sensory properties, and health benefits. Foods, 13 (6), 844. https://doi.org/10.3390/foods13060844
• King, J., Leong, S.Y., Alpos, M., Johnson, C., McLeod, S., Peng, M., Sutton, K., & Oey, I. (2024).Role of food processing and incorporating legumes in food products to increase protein intake and enhance satiety. Trends in Food Science & Technology, 147, 104466. https://doi.org/10.1016/j.tifs.2024.104466
• Lawless, H.T., & Heymann, H. (2010). Sensory evaluation of food: principles and practices (2nd ed.). Springer, Springer New York, USA.
• Ling, L., Loong, C., & Loke, W.M. (2021). Food additives in commercial cocoa beverage products and their effects on total polyphenol contents, cellular antioxidant and anti-inflammatory activities. Current Research in Nutrition and Food Science Journal, 9 (1), 20-30. https://dx.doi.org/10.12944/CRNFSJ.9.1.03
• Liu, Y., Toro-Gipson, R.S.D., & Drake, M. (2021). Sensory properties and consumer acceptance of ready-to-drink vanilla protein beverages. Journal of Sensory Studies, 36 (6), e12704. https://doi.org/10.1111/joss.12704
• Lu, Z.X., He, J.F., Zhang, Y.C., & Bing, D.J. (2020). Composition, physicochemical properties of pea protein and its application in functional foods. Critical Reviews in Food Science and Nutrition, 60 (15), 2593-2605. https://doi.org/10.1080/10408398.2019.1651248
• Mantzourani, I., Terpou, A., Bekatorou, A., Mallouchos, A., Alexopoulos, A., Kimbaris, A., Bezirtzoglou, E., Koutinas, A.A., & Plessas, S. (2020). Functional pomegranate beverage production by fermentation with a novel synbiotic L. paracasei biocatalyst. Food Chemistry, 308, 125658. https://doi.org/10.1016/j.foodchem.2019.125658
• Munekata, P.E.S., Pérez-Álvarez, J.A., Pateiro, M., Viuda-Matos, M., Fernández-López, J., & Lorenzo, J.M. (2021). Satiety from healthier and functional foods. Trends in Food Science & Technology, 113, 397-410. https://doi.org/10.1016/j.tifs.2021.05.025
• Myers, R.H., Montgomery, D.C., & Anderson-Cook, C.M. (2016). Response surface methodology: Process and product optimization using designed experiments (4th ed.). John Wiley & Sons, Inc, USA. pp 825.
• Nam, S.-H., Wagh, A., Martini, S., & Walsh, M.K. (2017). Sensory characterisation of a high-protein beverage. International Journal of Dairy Technology, 70 (3), 432-438. https://doi.org/10.1111/1471-0307.12362
• Oba, Ş., & Yılmaz, O. (2022). Effects of different prebiotic agents on the color and sensory properties of chocolate powder drinks (in Turkish). Harran Journal of Agricultural and Food Science, 26 (4), 501-518. https://doi.org/10.29050/harranziraat.1100382
• Oba, Ş., & Yılmaz, O. (2023). Optimization of cocoa powder beverages in terms of physical properties of prebiotic combination (in Turkish). Journal of Engineering Sciences and Design, 11 (3), 1054-1065. https://doi.org/10.21923/jesd.1094259
• Obayomi, O.V., Olaniran, A.F., & Owa, S.O. (2024). Unveiling the role of functional foods with emphasis on prebiotics and probiotics in human health: A review. Journal of Functional Foods, 119, 106337. https://doi.org/10.1016/j.jff.2024.106337
• Oliveira, D.F., Granato, D., & Barana, A.C. (2018). Development and optimization of a mixed beverage made of whey and water-soluble soybean extract flavored with chocolate using a simplex-centroid design. Food Science and Technology, 38 (3), 413-420. https://doi.org/10.1590/1678-457X.07017
• Oltman, A.E., Lopetcharat, K., Bastian, E., & Drake, M.A. (2015). Identifying key attributes for protein beverages. Journal of Food Science, 80 (6), 1383-S1390. https://doi.org/10.1111/1750-3841.12877
• Pérez-Marroquín, X.A., Estrada-Fernández, A.G., García-Ceja, A., Aguirre-Álvarez, G., & León-López, A. (2023). Agro-food waste as an ingredient in functional beverage processing: sources, functionality, market and regulation. Foods, 12 (8), 1583. https://doi.org/10.3390/foods12081583
• Plasek, B., Lakner, Z., Kasza, G., & Temesi, Á. (2019). Consumer evaluation of the role of functional food products in disease prevention and the characteristics of target groups. Nutrients, 12 (1), 69. https://doi.org/10.3390/nu12010069
• Pérez-Ramírez, I.F., Cariño-Sarabia, A., Castaño-Tostado, E., Vázquez-Landaverde, P.A., Ramos-Gómez, M., Reynoso-Camacho, R., & Amaya-Llano, S.L. (2021). Chemical and sensorial characterization of Tejate, a Mexican traditional maize-cocoa beverage, and improvement of its nutritional value by protein addition. Journal of Food Science and Technology, 58 (9), 3548-3560. https://doi.org/10.1007/s13197-021-05073-w
• Quant, A.J.B., Albis, A.R., & Perez, J.A. (2019). Effect of heat treatment on physical properties of whey protein beverages (WPB). Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry, 20, 209-218.
• Sadler, I., Bauer, A., & Kassam, S. (2024). Dietary habits and self-reported health outcomes in a cross-sectional survey of health-conscious adults eating a plant-based diet. Journal of Human Nutrition and Dietetics, 37 (4), 1061-1074. https://doi.org/10.1111/jhn.13321
• Saubenova, M., Oleinikova, Y., Rapoport, A., Maksimovich, S., Yermekbay, Z., & Khamedova, E. (2024). Bioactive peptides derived from whey proteins for health and functional beverages. Fermentation, 10 (7), 359. https://doi.org/10.3390/fermentation10070359
• Silva, J.D.C., Anghinoni, I.C.A.B., & Gomes, M.B. (2024). Plant-based food for the prevention of Type 2 diabetes: Scoping review. Nutrients, 16 (11), 1671. https://doi.org/10.3390/nu16111671
• Singh, J., Prakash, S., Bhandari, B., & Bansal, N. (2020). Ultra-high temperature (UHT) stability of chocolate flavored high protein beverages. Journal of Food Science, 85 (10), 3012-3019. https://doi.org/10.1111/1750-3841.15397
• Tan, M., Nawaz, M.A., & Buckow, R. (2023). Functional and food application of plant proteins – a review. Food Reviews International, 39 (5), 2428-2456. https://doi.org/10.1080/87559129.2021.1955918
• Thakur, A., Pandey, P., Dalbhagat, C.G., & Mishra, H.N. (2022). Development of grain-based carbonated beverage premix using maize (Zea mays), Bengal gram (Cicer arietinum), and finger millet (Eleusine coracana). Journal of Food Science and Technology, 59 (4), 1637-1648. https://doi.org/10.1007/s13197-021-05175-5
• Tireki, S. (2021). A review on packed non-alcoholic beverages: Ingredients, production, trends and future opportunities for functional product development. Trends in Food Science & Technology, 112, 442-454. https://doi.org/10.1016/j.tifs.2021.03.058
• Tjahyo, A.S., Wu, J.Y., Smith, G., Acuin, C., Maier, A.B., Sim, S.Y.J., Taneja, R., Haldar, S., & Henry, C.J. (2024). Shifting trend of protein consumption in southeast asia: toward health, ınnovation, and sustainability. Current Developments in Nutrition, 8 (10), 104443. https://doi.org/10.1016/j.cdnut.2024.104443
• Topolska, K., Florkiewicz, A., & Filipiak-Florkiewicz, A. (2021). Functional food—consumer motivations and expectations. International Journal of Environmental Research and Public Health, 18 (10), 5327. https://doi.org/10.3390/ijerph18105327
• Trikusuma, M., Paravisini, L., & Peterson, D.G. (2020). Identification of aroma compounds in pea protein UHT beverages. Food Chemistry, 312, 126082. https://doi.org/10.1016/j.foodchem.2019.126082
• Vasquez-Orejarena, E., Simons, C.T., Litchfield, J.H., & Alvarez, V.B. (2018). Functional properties of a high protein beverage stabilized with oat-β-glucan. Journal of Food Science, 83 (5), 1360-1365. https://doi.org/10.1111/1750-3841.14119
• Wanyonyi, M., Kiluyi, J.W., & Okango, A.A. (2025). Optimization of a functional beverage formulation using a constrained mixture design with multiple responses. Asian Journal of Probability and Statistics, 27 (1), 1-16. https://doi.org/10.9734/ajpas/2025/v27i1699
• Yılmaz Ersan, L., & Topçuoğlu, E. (2019). Microbiological and some physico-chemical properties of probiotic yogurt enriched with almond milk (in Turkish). Journal of Agricultural Faculty of Bursa Uludag University, 33 (2), 321-339.
• Yuan, L., Jiang, Q.Q., Zhai, Y., Zhao, Z., Liu, Y., Hu, F., & Sun, J. (2024). Association between plant-based diet and risk of chronic diseases and all-cause mortality in centenarians in China: A cohort study. Current Developments in Nutrition, 8 (1), 102065. https://doi.org/10.1016/j.cdnut.2023.102065