Ateş Dikeni (Pyracantha coccinea) Meyve ve Yaprak Ekstrelerinin, Antienzim, DNA koruma/kırılma ve Antimikrobiyal Aktivitesinin Araştırılması

Yıl 2025, Cilt: 8 Sayı: 1, 67 - 81, 17.01.2025

Merve Balaban , Şule Azime Yeniçeri , Ebru Akkemik , Bülent Hallaç , Mehmet Fidan

https://doi.org/10.47495/okufbed.1481421

Öz

Ateş dikeni (Pyracantha coccinea) bitkisi sahip olduğu sekonder metabolitler sayesinde birçok hastalığın tedavisinde kullanılma potansiyeli taşımaktadır. Ancak literatürde P. coccinea’nın farmakolojide kullanımı ile ilgili çok az sayıda çalışma vardır. Bu nedenle bu çalışmada P. coccinea’nın meyve ve yaprak metanol ekstreleri hazırlanarak antienzim aktivitesi in vitro şartlarda araştırılmak istenmiştir. Bu sebeple kanser tanı ve tedavisi ile ilişkisi olduğu literatürlerce bilinen Glutatyon-S transferaz (GST) ve Tirozinaz (TYR) enzim aktiviteleri üzerine P. coccinea’nın yaprak ve meyve ekstresinin etkisi belirlenmiştir. İlk kez bu çalışmada P. coccinea’nin meyve ve yaprak metanol ekstreleri DNA koruma/kırılma etkisi ve her iki ekstrenin Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29242), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231) mikroorganizmalarına karşı antimikrobiyal etkinliği araştırılmıştır. P. coccinea’nın yaprak ekstresinin GST’yi mikromolar düzeyde inhibe ettiği tespit edilmiştir. Her iki ekstrenin TYR enzim aktivitesi üzerinde yeterli etki göstermediği belirlenmiştir. Ayrıca ekstrelerin DNA kırılmasına yol açmadığı ve 1 mg/mL konsantrasyonda koruma etkisine sahip olduğu gözlemlenmiştir. Ancak ekstreler çalışılan tüm mikroorganizmalara karşı antimikrobiyal etkinliğe sahip olmadığı görülmüştür. Sonuç olarak P. coccinea’nın bitkisinin aday ilaç çalışmaları için doğal kaynak olabileceğini buna karşın ilave preklinik ve klinik çalışmalarla bitkinin tam potansiyelinin ortaya çıkarılması gerektiği düşünülmektedir.

Anahtar Kelimeler

Tirozinaz, Glutatyon S Transferaz, Enzim aktivitesi

Investigation of Antienzyme, DNA protection/breaking and Antimicrobial Activity of Firethorn (Pyracantha coccinea) Fruit and Leaf Extracts

Öz

Firethorn (Pyracantha coccinea) plant has the potential to be used in the treatment of many diseases thanks to its secondary metabolites. However, there are very few studies in the literature on the use of P. coccinea in pharmacology. Therefore, in this study, we aimed to investigate the antienzyme activity of P. coccinea under in vitro conditions by preparing fruit and leaf methanol extracts. The effect of P. coccinea leaf and fruit extract on Glutathione-S transferase (GST) and Tyrosinase (TYR) enzyme activities, which are known in the literature to be related to cancer diagnosis and treatment, was investigated. In this study, for the first time, the DNA protection/breaking effect of fruit and leaf methanol extracts of P. coccinea was investigated. Additionally, the antimicrobial activity of both extracts against Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29242), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231) microorganisms was investigated. It was determined that P. coccinea leaf extract inhibited GST at micromolar level. It was determined that both extracts did not have a sufficient effect on TYR enzyme activity. It was determined that the extracts did not cause DNA breakage and had a protection effect of 1 mg/mL concentration. However, it did not show antimicrobial activity against all microorganisms studied. As a result, we think that P. coccinea plant can be a natural source for candidate drug studies, but the full potential of the plant should be revealed with additional preclinical and clinical studies.

Anahtar Kelimeler

Tyrosinase, Glutathione-S transferase, Enzyme Activity

Kaynakça

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