Yıl 2021, Cilt 14 , Sayı 2, Sayfalar 338 - 345 2021-04-01

Kafein sıçan yavrularında bağırsak hasarı gelişiminde predispozan bir risk faktörü mü?
Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?

Özmert M.a. ÖZDEMİR [1] , Savaş SALDIRAY [2] , Yaşar ENLİ [3] , Nilay TÜRK [4] , Hacer ERGİN [5]


Amaç: Hipoksi/reoksijenizasyon (H/R) ile intestinal hasarlanma oluşturulan sıçan yavrularında kafein sitratın histopatolojik ve biyokimyasal etkilerini araştırmak. Gereç ve yöntem: Bir günlük 32 Wistar albino sıçan yavrusu rastgele dört gruba ayrıldı: kontrol grubu (grup1, n=8), kafein grubu (grup2, subkutan kafein sitrat uygulanan, n=8), H/R grubu (grup3, H/R uygulanan, n=8) ve kafein+H/R grubu (grup4, kafein sitrat verilen ve H/R uygulanan, n=8). Kafein sitrat, 20 mg/kg'lık bir yükleme dozunda başlatıldı, ardından subkutan 5 mg/kg/günlük idame dozu takip edildi. Dördüncü günde, grup 1 ve 2 dışındaki tüm hayvanlar H/R'ye maruz bırakıldı ve H/R prosedüründen 6 saat sonra öldürüldü. Histopatolojik hasarlanma skorları (HIS), interlökin-6 (IL-6), tümör nekroz faktörü-alfa (TNF-α) ve oksidatif stres indeksi (OSI: toplam oksidan durum “TOS”/toplam antioksidan durum “TAS”) seviyeleri bağırsak örnekleri üzerinden değerlendirildi. Bulgular: Histopatolojik olarak en ciddi hasar H/R uygulanan gruplarda görüldü (p <0.01). Kafein grubunun ortalama HIS'leri kontrol grubundan daha yüksek iken, H/R grubundan daha düşüktü, ancak bu istatistiksel olarak anlamlı değildi. Grup 2, 3 ve 4'teki TNF-α, IL-6 ve OSI düzeyleri kontrol grubundan anlamlı olarak yüksekti (p <0.05). Bununla birlikte, kafein grubundaki bu biyokimyasal parametreler, H/R uygulanan gruplardan anlamlı olarak daha düşüktü (p <0.01). Sonuç: Bu çalışma, kafein sitratın TNF-α, IL-6 ve OSI'nin bağırsak doku düzeylerini önemli ölçüde arttırdığını gösterdi. Bu nedenle, kafeinin bağırsak hasarı geliştirmek için predispozan bir risk faktörü olabileceğini düşünüyoruz.

Purpose: To investigate of histopathologic and biochemical effects of caffeine citrate in newborn rats with hypoxia/reoxygenation (H/R)-induced intestinal injury.
Materials and methods: One-day-old, 32 Wistar albino newborn rats (n=8) were randomly divided into four groups: control group (group1, n=8), caffeine group (group2, caffeine citrate administered subcutaneously, n=8), H/R group (group3, exposed to H/R, n=8), and caffeine + H/R group (group4, caffeine citrate administered and exposed to H/R, n=8). Caffeine citrate was initiated at a loading dose of 20 mg/kg, followed by a maintenance dose of 5 mg/kg/day, subcutaneously. On day 4th, all animals except for groups 1 and 2 were exposed to H/R and sacrificed 6 hours after H/R procedure. Histopathological injury scores (HISs), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and oxidative stress index (OSI: total oxidant status “TOS”/total antioxidant status “TAS”) levels were measured in intestinal samples.
Results: As histopathological, the most severe damage was observed in H/R-induced groups (p<0.01). Although not statistically significant, the mean HISs of caffeine group was higher than the control group and lower than the H/R group. The levels of TNF-α, IL-6 and OSI in the groups 2, 3 and 4 were significantly higher than the control group (p<0.05). However, these biochemical parameters in the caffeine group were significantly lower than those of the H/R-induced groups (p<0.01).
Conclusion: This study showed that caffeine citrate significantly increased the intestinal tissue levels of TNF-α, IL-6, and OSI. As a result, caffeine may be a predisposing risk factor for developing intestinal injury.   

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Birincil Dil en
Konular Pediatri
Yayınlanma Tarihi Nisan 2021
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-2499-4949
Yazar: Özmert M.a. ÖZDEMİR (Sorumlu Yazar)
Kurum: Pamukkale Ünv. Tıp Fak. Çocuk Sağlığı ve Hastalıkları, Neonatoloji Bilim Dalı
Ülke: Turkey


Orcid: 0000-0003-0437-9305
Yazar: Savaş SALDIRAY
Kurum: ŞANLIURFA BİRECİK DEVLET HASTANESİ
Ülke: Turkey


Orcid: 0000-0001-5080-3192
Yazar: Yaşar ENLİ
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-8294-558X
Yazar: Nilay TÜRK
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-6002-4202
Yazar: Hacer ERGİN
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Destekleyen Kurum Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Kurulu
Proje Numarası 2017TPF011
Teşekkür Yazarlar olarak Barbaros Şahin ve Pamukkale Üniversitesi Hayvan Araştırmaları Laboratuvarı'na yardımları için çok teşekkür ederiz
Tarihler

Yayımlanma Tarihi : 1 Nisan 2021

Bibtex @araştırma makalesi { patd804703, journal = {Pamukkale Tıp Dergisi}, issn = {}, eissn = {1308-0865}, address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli}, publisher = {Pamukkale Üniversitesi}, year = {2021}, volume = {14}, pages = {338 - 345}, doi = {10.31362/patd.804703}, title = {Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?}, key = {cite}, author = {Özdemir, Özmert M.a. and Saldıray, Savaş and Enli, Yaşar and Türk, Nilay and Ergin, Hacer} }
APA Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . (2021). Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? . Pamukkale Tıp Dergisi , 14 (2) , 338-345 . DOI: 10.31362/patd.804703
MLA Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?" . Pamukkale Tıp Dergisi 14 (2021 ): 338-345 <https://dergipark.org.tr/tr/pub/patd/issue/60284/804703>
Chicago Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?". Pamukkale Tıp Dergisi 14 (2021 ): 338-345
RIS TY - JOUR T1 - Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? AU - Özmert M.a. Özdemir , Savaş Saldıray , Yaşar Enli , Nilay Türk , Hacer Ergin Y1 - 2021 PY - 2021 N1 - doi: 10.31362/patd.804703 DO - 10.31362/patd.804703 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 338 EP - 345 VL - 14 IS - 2 SN - -1308-0865 M3 - doi: 10.31362/patd.804703 UR - https://doi.org/10.31362/patd.804703 Y2 - 2020 ER -
EndNote %0 Pamukkale Tıp Dergisi Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? %A Özmert M.a. Özdemir , Savaş Saldıray , Yaşar Enli , Nilay Türk , Hacer Ergin %T Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? %D 2021 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 14 %N 2 %R doi: 10.31362/patd.804703 %U 10.31362/patd.804703
ISNAD Özdemir, Özmert M.a. , Saldıray, Savaş , Enli, Yaşar , Türk, Nilay , Ergin, Hacer . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?". Pamukkale Tıp Dergisi 14 / 2 (Nisan 2021): 338-345 . https://doi.org/10.31362/patd.804703
AMA Özdemir Ö , Saldıray S , Enli Y , Türk N , Ergin H . Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?. Pam Tıp Derg. 2021; 14(2): 338-345.
Vancouver Özdemir Ö , Saldıray S , Enli Y , Türk N , Ergin H . Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?. Pamukkale Tıp Dergisi. 2021; 14(2): 338-345.
IEEE Ö. Özdemir , S. Saldıray , Y. Enli , N. Türk ve H. Ergin , "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?", Pamukkale Tıp Dergisi, c. 14, sayı. 2, ss. 338-345, Nis. 2021, doi:10.31362/patd.804703