Purpose: To investigate of histopathologic and biochemical effects of caffeine citrate in newborn rats with hypoxia/reoxygenation (H/R)-induced intestinal injury.
Materials and methods: One-day-old, 32 Wistar albino newborn rats (n=8) were randomly divided into four groups: control group (group1, n=8), caffeine group (group2, caffeine citrate administered subcutaneously, n=8), H/R group (group3, exposed to H/R, n=8), and caffeine + H/R group (group4, caffeine citrate administered and exposed to H/R, n=8). Caffeine citrate was initiated at a loading dose of 20 mg/kg, followed by a maintenance dose of 5 mg/kg/day, subcutaneously. On day 4th, all animals except for groups 1 and 2 were exposed to H/R and sacrificed 6 hours after H/R procedure. Histopathological injury scores (HISs), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and oxidative stress index (OSI: total oxidant status “TOS”/total antioxidant status “TAS”) levels were measured in intestinal samples.
Results: As histopathological, the most severe damage was observed in H/R-induced groups (p<0.01). Although not statistically significant, the mean HISs of caffeine group was higher than the control group and lower than the H/R group. The levels of TNF-α, IL-6 and OSI in the groups 2, 3 and 4 were significantly higher than the control group (p<0.05). However, these biochemical parameters in the caffeine group were significantly lower than those of the H/R-induced groups (p<0.01).
Conclusion: This study showed that caffeine citrate significantly increased the intestinal tissue levels of TNF-α, IL-6, and OSI. As a result, caffeine may be a predisposing risk factor for developing intestinal injury.
| Birincil Dil | en |
|---|---|
| Konular | Pediatri |
| Yayınlanma Tarihi | Nisan 2021 |
| Bölüm | Araştırma Makalesi |
| Yazarlar |
|
| Destekleyen Kurum | Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Kurulu |
| Proje Numarası | 2017TPF011 |
| Teşekkür | Yazarlar olarak Barbaros Şahin ve Pamukkale Üniversitesi Hayvan Araştırmaları Laboratuvarı'na yardımları için çok teşekkür ederiz |
| Tarihler |
Yayımlanma Tarihi : 1 Nisan 2021 |
| Bibtex | @araştırma makalesi { patd804703,
journal = {Pamukkale Tıp Dergisi},
issn = {},
eissn = {1308-0865},
address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli},
publisher = {Pamukkale Üniversitesi},
year = {2021},
volume = {14},
pages = {338 - 345},
doi = {10.31362/patd.804703},
title = {Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?},
key = {cite},
author = {Özdemir, Özmert M.a. and Saldıray, Savaş and Enli, Yaşar and Türk, Nilay and Ergin, Hacer}
} |
| APA | Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . (2021). Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? . Pamukkale Tıp Dergisi , 14 (2) , 338-345 . DOI: 10.31362/patd.804703 |
| MLA | Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?" . Pamukkale Tıp Dergisi 14 (2021 ): 338-345 <https://dergipark.org.tr/tr/pub/patd/issue/60284/804703> |
| Chicago | Özdemir, Ö , Saldıray, S , Enli, Y , Türk, N , Ergin, H . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?". Pamukkale Tıp Dergisi 14 (2021 ): 338-345 |
| RIS | TY - JOUR T1 - Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? AU - Özmert M.a. Özdemir , Savaş Saldıray , Yaşar Enli , Nilay Türk , Hacer Ergin Y1 - 2021 PY - 2021 N1 - doi: 10.31362/patd.804703 DO - 10.31362/patd.804703 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 338 EP - 345 VL - 14 IS - 2 SN - -1308-0865 M3 - doi: 10.31362/patd.804703 UR - https://doi.org/10.31362/patd.804703 Y2 - 2020 ER - |
| EndNote | %0 Pamukkale Tıp Dergisi Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? %A Özmert M.a. Özdemir , Savaş Saldıray , Yaşar Enli , Nilay Türk , Hacer Ergin %T Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats? %D 2021 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 14 %N 2 %R doi: 10.31362/patd.804703 %U 10.31362/patd.804703 |
| ISNAD | Özdemir, Özmert M.a. , Saldıray, Savaş , Enli, Yaşar , Türk, Nilay , Ergin, Hacer . "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?". Pamukkale Tıp Dergisi 14 / 2 (Nisan 2021): 338-345 . https://doi.org/10.31362/patd.804703 |
| AMA | Özdemir Ö , Saldıray S , Enli Y , Türk N , Ergin H . Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?. Pam Tıp Derg. 2021; 14(2): 338-345. |
| Vancouver | Özdemir Ö , Saldıray S , Enli Y , Türk N , Ergin H . Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?. Pamukkale Tıp Dergisi. 2021; 14(2): 338-345. |
| IEEE | Ö. Özdemir , S. Saldıray , Y. Enli , N. Türk ve H. Ergin , "Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?", Pamukkale Tıp Dergisi, c. 14, sayı. 2, ss. 338-345, Nis. 2021, doi:10.31362/patd.804703 |
