Soluble green tea production and determination of changes during the in vitro gastrointestinal system

Yıl 2025, Cilt: 62 Sayı: 4, 451 - 464, 12.12.2025

Merve İnce Palamutoğlu , Recep Palamutoğlu , Cemal Kasnak

https://doi.org/10.20289/zfdergi.1655525

Öz

Objective: This study investigated the transformations and bio-accessibility of soluble green tea components throughout the digestive process by utilizing an in vitro gastrointestinal system simulation.
Material and Methods: Green tea was obtained from a local market considering the expiration date. In preliminary experiments, it was determined that the highest phenolic content was reached when 2% infusions were brewed at 80ºC for 60 seconds. The prepared infusions were filtered, maltodextrin was added and freeze-dried at -59.5ºC, 33 Pa for 48 hours. Color, total phenolic substance and antioxidant activity analyses were performed on green tea infusion (GT) and maltodextrin-coated green tea (SGT). In addition, changes in phenolic substance during the in vitro gastrointestinal process were investigated by HPLC.
Results: In the initial condition, only catechin and epicatechin contents were statistically significant (p<0.01) when comparing GT and SGT. Phenolic acid content, antioxidant activity, and phenolic compounds showed statistically significant differences in different regions of the gastrointestinal system. Additionally, changes in both green tea samples throughout the digestive process were statistically significant (p<0.01).
Conclusion: Significant alterations in phenolic content, antioxidant capacity, and polyphenolic composition were observed in GT and SGT samples; co-application of maltodextrin with other materials may enhance green tea phenolic stability and bioavailability.

Anahtar Kelimeler

DPPH , extraction , flavonoids , gastrointestinal system , green tea , in vitro polyphenols

Etik Beyan

Ethics approval was obtained from the Afyonkarahisar Health Sciences University Non-Interventional Scientific Research Ethics Committee (dated 1 September 2023 and no. 2023/401).

Destekleyen Kurum

This research was funded by the Afyonkarahisar Health Sciences University of BAP commission with project number 23.GENEL.025.

Proje Numarası

This research was funded by the Afyonkarahisar Health Sciences University of BAP commission with project number 23.GENEL.025.

Çözünebilir yeşil çay üretimi ve in vitro gastrointestinal süreçteki değişikliklerin belirlenmesi

Öz

Amaç: Bu çalışma, çözünebilir yeşil çay bileşenlerinin sindirim sürecindeki dönüşümlerini ve biyoyararlanımını in vitro gastrointestinal sistem simülasyonu ile incelemiştir.
Materyal ve Yöntem: Yeşil çay, son kullanma tarihi dikkate alınarak yerel bir marketten temin edilmiştir. Ön deneylerde, 2% infüzyonların 80ºC’de 60 saniye demlendiğinde en yüksek fenolik içeriğe ulaştığı belirlenmiştir. Hazırlanan infüzyonlar süzülerek maltodekstrin eklenmiş ve -59,5ºC, 33 Pa’da 48 saat boyunca dondurarak kurutulmuştur. Yeşil çay infüzyonu (YÇ) ve maltodekstrin kaplı yeşil çayın (MYÇ) renk, toplam fenolik madde ve antioksidan aktivite analizleri yapılmıştır. Ayrıca, fenolik madde değişimi HPLC ile değerlendirilmiştir.
Araştırma Bulguları: Başlangıçta, YÇ ve MYÇ arasındaki kateşin ve epikateşin içerikleri istatistiksel olarak anlamlı bulunmuştur (p<0.01). Gastrointestinal sistemin farklı bölgelerinde fenolik asit içeriği, antioksidan aktivite ve fenolik bileşiklerde önemli farklılıklar gözlenmiştir. Sindirim sürecinde her iki çay örneğinde de belirgin değişimler meydana gelmiştir (p<0.01).
Sonuç: YÇ ve MYÇ örneklerinde fenolik içerik, antioksidan kapasite ve polifenolik bileşimde önemli değişiklikler gözlemlendi; maltodekstrinin diğer materyallerle birlikte uygulanması yeşil çay fenolik stabilitesini ve biyoyararlanımını iyileştirebilir.

Anahtar Kelimeler

DPPH , ekstraksiyon , flavonoid , gastrointestinal sistem , yeşil çay , in vitro fenolik

Etik Beyan

Ethics approval was obtained from the Afyonkarahisar Health Sciences University Non-Interventional Scientific Research Ethics Committee (dated 1 September 2023 and no. 2023/401).

Destekleyen Kurum

This research was funded by the Afyonkarahisar Health Sciences University of BAP commission with project number 23.GENEL.025.

Proje Numarası

This research was funded by the Afyonkarahisar Health Sciences University of BAP commission with project number 23.GENEL.025.

Kaynakça

• Akbulut, A., Ş.M. Kara & A. Özcan, 2020. Comparison of black, green and white teas in terms of quality criteria, mineral contents, antioxidant and antimicrobial activity. Academic Journal of Agriculture, 9 (2): 279-288. https://doi.org/10.29278/azd.720699
• Baş, M., 2019. Life is 9 Meters. 1st edition. İstanbul: Destek Publishing Ltd. Co., 559 pp.
• Brand-Williams, W., M.E. Cuvelier & C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28 (1): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
• Brodkorb, A., L. Egger, M. Alminger, P. Alvito, R. Assunção, S. Ballance, T. Bohn, C. Bourlieu-Lacanal, R. Boutrou, F. Carrière, A. Clemente, M. Corredig, D. Dupont, C. Dufour, C. Edwards, M. Golding, S. Karakaya, B. Kirkhus, S. Le Feunteun, U. Lesmes, A. Macierzanka, A.R. Mackie, C. Martins, S. Marze, D.J. McClements, O. Ménard, M. Minekus, R. Portmann, C.N. Santos, I. Souchon, R.P. Singh, G.E. Vegarud, M.S.J. Wickham, W. Weitschies & I. Recio, 2019. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nat Protoc., 14: 991-1014 pp. https://doi.org/10.1038/s41596-018-0119-1
• Chuysinuan, P., N. Chunshom, P. Kotcharat, T. Thanyacharoen, S. Techasakul & Sarute Ummartyotin, 2021. The encapsulation of green tea extract in cyclodextrin and loading into chitosan‑based composites: controlled‑release behavior and antioxidant properties. Journal of Polymers and the Environment, 29: 2628-2638. https://doi.org/10.1007/s10924-021-02058-w
• Çomak Göçer, E.M., F. Ergin, I. Özen Küçükçetin & A. Küçükçetin, 2021. In vitro gastrointestinal resistance of Lactobacillus acidophilus in some dairy products. Brazilian Journal of Microbiology, 52 (4): 2319-2334. https://doi.org/10.1007/s42770-021-00590-4
• Çomak Göçer, E.M., F. Zeren Ergin, I. Özen Küçükçetin & A. Küçükçetin, 2024. Rheological, microbiological, and in vitro digestive properties of kefir produced with different kefir grains and commercial starter cultures. Food Science and Technology International, https://doi.org/10.1177/10820132241304130
• Cruz-Molina, A.V.D.L., J.F. Ayala Zavala, A.T. Bernal Mercado, M.R. Cruz Valenzuela, G.A. González-Aguilar, J. Lizardi- Mendoza, F. Brown-Bojorquez & B.A. Silva-Espinoza, 2021. Maltodextrin encapsulation improves thermal and pH stability of green tea extract catechins. Journal of Food Processing and Preservation, 45 (9). https://doi.org/10.1111/jfpp.15729
• Friedman, M. & H.S. Jürgens, 2000. Effect of pH on the stability of plant phenolic compounds. Journal of Agricultural and Food Chemistry, 48: 2101-2110. https://doi.org/10.1021/jf990489j
• Fusco, V., F. Fanelli & D. Chieffi, 2022. Authenticity of probiotic foods and dietary supplements: A pivotal issue to address. Critical Reviews in Food Science and Nutrition, 62 (25): 6854-6871. https://doi.org/10.1080/10408398.2021.1907300
• Henning, S.M., C. Fajardo-Lira, H.W. Lee, A.A. Youssefian, V.L.W. Go & D. Heber, 2003. Catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity. Nutrition and Cancer, 45 (2): 226-235. https://doi.org/10.1207/S15327914NC4502_13
• HunterLab., 2001. Hunter L*a*b* Color Scale. Applications Note-Insight on Color, HunterLab. 13 (2): 1-4
• IBM Corp., 2019. IBM SPSS Statistics for Windows. Version 26.0. Armonk, NY. (Computer software)
• İnce-Palamutoğlu, M., G. Köse & M. Baş, 2023. Determination of the viability of lactic acid bacteria by dynamic in vitro gastrointestinal model in household and industrial-type kefir samples. Nutrients, 15 (22): 4808. https://doi.org/10.3390/nu15224808
• Jang, M., R. Park, Y.I. Park, Y.E. Cha, A. Yamamoto, J.I. Lee & J. Park, 2021. EGCG, a green tea polyphenol, inhibits human coronavirus replication in vitro, Biochemical and Biophysical Research Communications 547: 23-28. https://doi.org/10.1016/j.bbrc.2021.02.016
• Khatoon, N., 2023. Benefits of green tea: A review. Journal of Pharmaceutical Sciences and Research, Lincoln University College, Malaysia, 10: 162-168. https://doi.org/10.31674/book.2023pcbs014
• Kızıl, M., 2019. “Food Consumption: Digestion, Absorption, Transport, and Excretion of Nutrients, 2-16”. In: Krause Food and the Nutrition Care Process (Eds. G. Akbulut). Ankara: Nobel Medical Bookstore Ltd. Co., 1152 pp.
• Li, C., W. Yu, P. Wu & X.D. Chen, 2020. Current in vitro digestion systems for understanding food digestion in human upper gastrointestinal tract. Trends in Food Science and Technology, 96: 114-126. https://doi.org/10.1016/j.tifs.2019.12.015
• LIczbínski, P. & B. Bukowska, 2022. Tea and coffee polyphenols and their biological properties based on the latest in vitro investigations. Industrial Crops & Products 175: 114265. https://doi.org/10.1016/j.indcrop.2021.114265
• Liu, G., W. Zhu, J. Zhang, D. Song, L. Zhuang, Q. Ma, X. Yang, X. Liu, J. Zhang, H. Zhang, J. Wang, L. Liang & X. Xu, 2022. Antioxidant capacity of phenolic compounds separated from tea seed oil in vitro and in vivo. Food Chemistry 371: 131122. https://doi.org/10.1016/j.foodchem.2021.131122
• Ma, B., J. Wang, C. Xu, Z. Wang, D. Yin, B. Zhou & C. Ma, 2022. Interrelation analysis between phenolic compounds and in vitro antioxidant activities in Pu-erh tea. LWT - Food Science and Technology 158: 113117. https://doi.org/10.1016/j.lwt.2022.113117
• Medfai, W., I. Oueslati, E. Dumas, Z. Harzalli, C. Viton, R. Mhamdi & A. Gharsallaoui, 2023. Physicochemical and biological characterization of encapsulated olive leaf extracts for food preservation. Antibiotics, 12 (6): 987. https://doi.org/10.3390/antibiotics12060987.hal-04131852
• Minekus, M., M. Alminger, P. Alvito, S. Ballance, T. Bohn, C. Bourlieu, F. Carrière, R. Boutrou, M. Corredig, D. Dupont, C. Dufour, L. Egger, M. Golding, S. Karakaya, B. Kirkhus, S. Le Feunteun, U. Lesmes, A. Macierzanka, A.R. Mackie, S. Marze, D.J. McClements, O. Ménard, I. Recio, C.N. Santos, R.P. Singh, G.E. Vegarud, M.S.J. Wickham, W. Weitschies & A. Brodkorb, 2014. A standardised static in vitro digestion method suitable for food-an international consensus. Food and Function, 5: 1113-1124. https://doi.org/10.1039/c3fo60702j
• Navarro-Flores, M.J., L.M.C. Ventura-Canseco, R. Meza-Gordillo, T. del R. Ayora-Talavera & M. Abud-Archila, 2020. Spray drying encapsulation of a native plant extract rich in phenolic compounds with combinations of maltodextrin and non-conventional wall materials. Journal of Food Science and Technology, 57 (11): 4111-4122. https://doi.org/10.1007/s13197-020-04447-w
• Parvez, S., I. Ahmed Wani & F.A. Masoodi, 2022. Nanoencapsulation of green tea extract using maltodextrin and its characterisation. Food Chemistry, 384: 132579. https://doi.org/10.1016/j.foodchem.2022.132579
• Prasanth, M.I., B.S. Sivamaruthi, C. Chaiyasut & T. Tencomnao, 2019. A review of the role of green tea (camellia sinensis) in antiphotoaging, stress resistance, neuroprotection, and autophagy. Nutrients, 11 (2): 474. https://doi.org/10.3390/nu11020474
• Radeva-Ilieva, M., S. Stoeva, N. Hvarchanova & K.D. Georgiev, 2025. Green tea: current knowledge and issues. Foods, 14: 475. https://doi.org/10.3390/foods14050745
• Saka, M., E. Köseler & S. Metin, 2016. “Gastrointestinal System Diseases and Nutritional Therapy, 541-638”. In: Nutritional Therapy in Diseases, 2nd Edition (Eds. E.M. Tüfekçi Alphan). Ankara: Hatipoğlu Publishing Ltd. Co., 1045 pp.
• Şatır, G., 2023. Determination of the effects of black and green tea use on kombucha fermentation. Journal of Agriculture Faculty of Ege University, 60 (3): 465-472. https://doi.org/10.20289/zfdergi.1333999
• Schanda, J., 2018. CIE 015:2018 Colorimetry, 4th Edition; Wiley & Sons, Inc., 459 pp. ISBN 9783902842138.
• Sensoy, I., 2021. A review on the food digestion in the digestive tract and the used in vitro models. Current Research in Food Science, 4: 308-319. https://doi.org/10.1016/j.crfs.2021.04.004.
• Silva, F.M.R., F.E.A. Magalhaes, F.L.A. Batista, L.M.R. da Silva, N.M.P. Silva-Ricardo, L.B. de Sousa-Sabino & R.W. dr Figueiredo, 2023. Microencapsulation of green tea (Camellia sinensis) phenolic extract: Physical-chemical characterization, antimicrobial and toxicological properties. Food Chemistry Advances 3: 100360. https://doi.org/10.1016/j.focha.2023.100360
• Singh, K., S. Srichairatanakool, T. Chewonarin, C.S. Brennan, M.A. Brennan, W. Klangpetch & N. Utama-Ang, 2022a. Manipulation of the Phenolic Quality of Assam Green Tea through Thermal Regulation and Utilization of Microwave and Ultrasonic Extraction Techniques. Horticulturae, 8 (4). https://doi.org/10.3390/horticulturae8040338
• Singh, V., H.W. Son, G.D. Lee, S. Lee, T. Unno & J.H. Shin, 2022b. Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut-microbial studies. Biotechnology and Bioengineering, 119 (11): 3044-3061. https://doi.org/10.1002/bit.28206
• Singleton, V. & J. Rossi, 1965. Colorimetry of Total Phenolic Compounds with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16: 144-158.
• Taş, S., E. Sarandol, S. Ziyanok, K. Aslan & M. Dirican, 2005. Effects of green tea on serum paraoxonase/arylesterase activities in streptozotocin induced diabetic rats. Nutrition Research, 25 (12): 1061-1074. https://doi.org/10.1016/j.nutres.2005.10.001
• Tengse, D.D., B. Priya, & P.A.R. Kumar, 2017. Optimization for encapsulation of green tea (Camellia sinensis L.) extract by spray drying technology. Journal of Food Measurement and Characterization, 11 (1): 85-92. https://doi.org/10.1007/s11694-016-9374-4
• Wang, H., G.J. Provan & K. Helliwell, 2000. Tea flavonoids: Their functions, utilisation and analysis. Trends Food Sci Technol, 11: 152-160. https://doi.org/10.1016/S0924-2244(00)00061-3
• Zhao, C.N., G.Y. Tang, S.Y. Cao, X.Y. Xu, R.Y. Gan, Q. Liu, Q.Q. Mao, A. Shang & L.H. Bin, 2019. Phenolic profiles and antioxidant activities of 30 tea infusions from green, black, oolong, white, yellow and dark teas. Antioxidants, 8 (7): 9-13. https://doi.org/10.3390/antiox8070215
• Zorzenon, M.R.T., M. Formigoni, S.B. da Silva, F. Hodas, S. Piovan, S.R. Ciotta, C.A. Jansen, A.S. Dacome, E.J. Pilau, C.E. Mareze-Costa, P.G. Milani & S.C. Costa, 2020. Spray drying encapsulation of stevia extract with maltodextrin and evaluation of the physicochemical and functional properties of produced powders. Journal of Food Sciences, 85 (10): 3590-3600. https://doi.org/10.1111/1750-3841.15437