The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue

Year 2022, Volume: 1 Issue: 1, 1 - 7, 30.12.2022

Ahmet Mentese Selim Demir Nihal Türkmen Yüksel Aliyazıcıoğlu Orhan Değer

Abstract

5-fluorouracil (5-FU) is a potent anticancer agent, but its significant tissue toxicity associated with increased oxidative stress and inflammation may limit its clinical use. Chlorogenic acid (CGA) is a dietary polyphenol found in a variety of foods and beverages, including coffee, apples, pears, strawberries, and grapes. It was aimed to evaluate the therapeutic effects of CGA on 5-FU-induced ovotoxicity through oxidative stress and inflammation parameters in this study. Thirty female Sprague-Dawley rats were divided into 5 groups with equal numbers of subjects (n=6): control, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) and CGA (3 mg/kg). The ovarian tissue levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), 8-hydroxy-2' -deoxyguanosine (8-OHdG), catalase (CAT) and interleukin-6 (IL-6) were determined using commercial spectrophotometric kits. While 5-FU treatment increased MDA, TOS, 8-OHdG and IL-6 levels in ovarian tissue, it significantly decreased TAS and CAT levels (p<0.05). These parameters, indicating 5-FU-induced toxicity, were significantly reversed with CGA administrations in a dose-dependent manner. The results support the view that CGA may be a useful modulator in attenuating 5-FU-induced ovotoxicity.

Keywords

5-fluorouracil, Chlorogenic acid, Inflammation, Ovotoxicity, Oxidative stress

Klorojenik Asidin Sıçan Yumurtalık Dokusunda 5-Florourasil ile İndüklenen Oksidatif Hasar Üzerine Etkisi

Abstract

5-fluorourasil (5-FU) güçlü bir antikanser ajandır, ancak artan oksidatif stres ve inflamasyon ile ilişkili önemli doku toksisitesi klinik kullanımını sınırlayabilmektedir. Klorojenik asit (CGA) kahve, elma, armut, çilek ve üzüm dahil olmak üzere çeşitli yiyecek ve içeceklerde bulunan bir diyet polifenolüdür. Bu çalışmada, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesi üzerine terapötik etkilerinin oksidatif stres ve inflamasyon parametreleri üzerinden değerlendirilmesi amaçlandı. Otuz Sprague-Dawley ırkı dişi sıçan eşit sayıda denek (n=6) içeren 5 gruba ayrıldı: kontrol, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) ve CGA (3 mg/kg). Yumurtalık dokularında malondialdehit (MDA), toplam oksidan durum (TOS), toplam antioksidan durum (TAS), 8-hidroksi-2'-deoksiguanozin (8-OHdG), katalaz (CAT) ve interlökin-6 (IL-6) seviyeleri ticari spektrofotometrik kitler kullanılarak belirlendi. 5-FU uygulaması yumurtalık dokusunda MDA, TOS, 8-OHdG ve IL-6 düzeylerini artırırken, TAS ve CAT düzeylerini anlamlı olarak azalttı (p<0.05). 5-FU ile indüklenen toksisiteyi gösteren bu parametreler, CGA tedavisi ile doza bağlı bir şekilde anlamlı derecede tersine çevrildi (p<0.05). Sonuçlar, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesini azaltmada faydalı bir modülatör olabileceği görüşünü desteklemektedir.

Keywords

5-florourasil, İnflamasyon, Klorojenik asit, Yumurtalık dokusu toksisitesi, Oksidatif stres

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