Changes in bioactive compounds and antioxidant activity of Gaziantep and Kastamonu garlic during black garlic production
Abstract
Garlic (Allium sativum L.), a member of the Alliaceae family, has been widely used in cuisine and traditional medicine since ancient times. Black garlic is produced by fermentation of fresh garlic under controlled conditions for a certain period at high temperature (60-90°C) and high humidity (70-90%). According to the Turkish Statistical Institute (TURKSTAT) data, Kastamonu and Gaziantep garlic varieties are the most cultivated garlic varieties in our country. Changes in protein, sugar content, antioxidant capacity (DPPH and ABTS methods), total phenolic content, 5-hydroxymethylfurfural (HMF) content, and organosulfur compound profiles were investigated in samples taken from Kastamonu and Gaziantep fresh garlic at 7, 14, 21, and 28 days of black garlic production under 65°C temperature and 70% humidity conditions. With these analyses, the differences between black garlic and fresh garlic and the changes in black garlic during the production process were revealed in detail. It was determined that the amount of total phenolic content and antioxidant capacities increased in the black garlic production processes of both regions compared to fresh garlic. While sucrose was fresh garlic’s dominant sugar, fructose was black garlic’s dominant sugar. Among the organosulfur compounds, allicin was dominant in fresh garlic and SAC in black garlic. It was determined that SAC was formed after the enzymatic conversion of ɣ-glutamyl-S-alk(en)yl-L-cysteine and ɣ-glutamyl and the temperature and fermentation time used in black garlic production increased the formation of SAC. The protein content ranging between 5.8%-7.3% in fresh garlic was 13.1-14.2% in black garlic. Fresh and black garlic from the Gaziantep region was determined to have higher total phenolic content, antioxidant capacity, and organosulfur compound contents.
Keywords
Black garlic, Antioxidant capacity, HMF, Organosulfur compounds
Project Number
This study was supported by TUBITAK (The Scientific and Technological Research Council of Turkey) within the scope of the 1001 project with project number "219O174".
References
- Abu-Lafi, S., Dembicki, J. W., Goldshlag, P., Hanuš, L. O., & Dembitsky, V. M. (2004). The use of the ‘Cryogenic ’GC/MS and on-column injection for study of organosulfur compounds of the Allium sativum. Journal of Food Composition and Analysis, 17(2), 235-245. https://doi.org/10.1016/j.jfca.2003.09.002
- AOAC, 1997. Official methods of analysis of AOAC International 16th ed., Association of Official Analytical Chemists, Washington, DC
- Atanasova-Goranova, V. K., Dimova, P. I., & Pevicharova, G. T. (1997). Effect of food products on endogenous generation of n− nitrosamines in rats. British Journal of Nutrition, 78(2), 335-345. https://doi.org/10.1079/BJN19970151
- Brand-Williams, W., Cuvelier, M.E., Berset, C.L.W.T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
- Choi, D. J., Lee, S. J., Kang, M. J., Cho, H. S., Sung, N. J., & Shin, J. H. (2008). Physicochemical characteristics of black garlic (Allium sativum L.). Journal of the Korean Society of Food Science and Nutrition, 37(4), 465-471.
- Condurso, C., Cincotta, F., Tripodi, G., Merlino, M., & Verzera, A. (2019). Influence of drying technologies on the aroma of Sicilian red garlic. Lwt, 104, 180-185. https://doi.org/10.1016/j.lwt.2019.01.026
- Haber‐Mignard, D., Suschetet, M., Bergès, R., Astorg, P., & Siess, M. H. (1996). Inhibition of aflatoxin B1‐and N‐nitrosodiethylamine‐induced liver preneoplastic foci in rats fed naturally occurring allyl sulfides. https://doi.org/10.1080/01635589609514428
- Hamma, I. L., Ibrahim, U., & Mohammed, A. B. (2013). Growth, yield, and economic performance of garlic (Allium sativum L.) as influenced by farm yard manure and spacing in Zaria, Nigeria. Journal of Agricultural Economics and Development, 2(1), 1-5.
- Hofmann, T., & Schieberle, P. (2000). Formation of aroma-active Strecker-aldehydes by a direct oxidative degradation of Amadori compounds. Journal of Agricultural and Food Chemistry, 48(9), 4301-4305. https://doi.org/10.1021/jf000076e
- Huang, C. H., Hsu, F. Y., Wu, Y. H., Zhong, L., Tseng, M. Y., Kuo, C. J., ... & Chiou, S. H. (2015). Analysis of the lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach. The Journal of Nutritional Biochemistry, 26(8), 808-817. https://doi.org/10.1016/j.jnutbio.2015.02.010
