Evaluation of Gibberellic Acid Pretreatment in the Alleviation of Salinity-induced Stress in Buckwheat Seeds

Year 2025, Volume: 9 Issue: 3, 900 - 905, 27.09.2025

Nilüfer Koçak , Onur Okumuş

https://doi.org/10.31015/2025.3.29

Abstract

This study investigated the effects of salinity stress on buckwheat (Fagopyrum esculentum) seed germination and the contribution of different gibberellic acid (GA₃) doses to mitigate this stress. Salinity is one of the major abiotic stress factors that negatively affect crop yield and quality worldwide. Improved seed germination has been achieved through various seed pre-treatment methods. Gibberellic acid is an important plant growth regulator that plays a role in the growth and development processes of many plant species. This research was conducted on the var. Aktas of buckwheat treated with three doses of (0, 5, 10, 20 dS m-1) of NaCl and post-treated with three different GA₃ doses (0, 100, 200 mg L-1). The study analyzed mean germination time, germination percentage, shoot and root length, fresh and dry weight data of the buckwheat seeds. The results revealed significant differences among salinity levels and alleviating effects of gibberellic acid post treatments on NaCl pretreated seedlings. It was observed that seed post-treatment with gibberellic acid positively affected germination parameters at increasing salt concentrations. The best results were observed in seedlings treated with 200 mg L-1 gibberellic acid on non-salt treated seeds under controlled conditions (0 dS m⁻¹, control).These findings suggest that gibberellic acid treatments have a potential to enhance buckwheat germination under salinity stress and could be used commercially.

Keywords

Abiotic stress , Gibberellic acid , Germination , Fagopyrum esculentum

Supporting Institution

Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara

References

• Abdel-Hamid, A.M., & Mohamed, H.I. (2014). The effect of the exogenous gibberellic acid on two salt stressed barley cultivars. European Scientific Journal, 10(6), 228-245.
• Anwar, T., Munwwar, F., Qureshi, H., Siddiqi, E. H., Hanif, A., Anwaar, S.,Gul, S., Waheed, A., Alwahibi, M.S., & Kamal, A. (2023). Synergistic effect of biochar-based compounds from vegetable wastes and gibberellic acid on wheat growth under salinity stress. Scientific Reports, 13(1), 19024.
• Arif, Y., Singh, P., Siddiqui, H., Bajguz, A., & Hayat, S. (2020). Salinity induced physiological and biochemical changes in plants: An omic approach towards salt stress tolerance. Plant Physiology and Biochemistry, 156, 64-77.
• Attia, H., Alamer, K., Algethami, B., Zorrig, W., Hessini, K., Gupta, K., & Gupta, B. (2022). Gibberellic acid interacts with salt stress on germination, growth and polyamine gene expression in fennel (Foeniculum vulgare Mill.) seedlings. Physiology and Molecular Biology of Plants, 28(3), 607-622.
• Chrungoo, N. K. and Chettry, U. (2021). Buckwheat: A critical approach towards assessment of its potential as a super crop. Indian Journal of Genetics and Plant Breeding, 81(01), 1-23.
• Day, S., & Koçak-Şahin, N. (2023). Seed Priming with MgCl2, CaCl2, and ZnCl2 as a Biofortification Based-Approach Induces Changes in Anise Seedlings Emergence. Phyton, (0031-9457), 92(8).
• Day, S., Koçak, N., & Önol, B. (2024). Hemp seed priming via different agents to alleviate temperature stress. Journal of Agricultural Sciences, 30(3), 562-569.
• El Sabagh, A., Islam, M. S., Skalicky, M., Ali Raza, M., Singh, K., Anwar Hossain, M., Hussain, A., Mahboob, W., Iqbal, M.A. Ratnasekera, D., Signhal, D., Signhal, R.K., Ahmed, S., Kumari, A., Wasaya, A., Sytar, O., Brectic, M., Çıg, F., Erman, M., Rahman, M.H., Ullah, N., & Arshad, A. (2021). Salinity stress in wheat (Triticum aestivum L.) in the changing climate: Adaptation and management stpercentagegies. Frontiers in Agronomy, 3, 661932.
• Essa, T.A., Salama, A.M., & El-Tayeb, M.A. (2019). Influence of gibberellic acid on seed germination of Hordeum vulgare under salt stress conditions. Journal of Crop Science, 30(2), 312-320.
• Gueridi, S., Boucelha, L., Abrous-Belbachir, O., & Djebbar, R. (2024). Effects of hormopriming and pretreatment with gibberellic acid on fenugreek (Trigonella foenum graecum L.) seed germination. Acta Botanica Croatica, 83(2), 0-0.
• Hayat, F., Khan, U., Zeeshan, M., Ali, Q., Nawaz, M. A., Ahmed, N., Ercişli, S., Kandhan, K., Al- Zayadneh, W., Sulieman, S., Sheteıwy, M., & Alyafei, M. (2024). Mechanistic understanding of hormone regulation of abiotic stresses in horticultural plants: A review. Turkish Journal of Agriculture and Forestry, 48(6), 825-840.
• Janah, I., Ben-Laouane, R., Elhasnaoui, A., Anli, M., Meddich, A. (2024). The induction of salt stress tolerance by gibberellic acid treatment in Stevia rebaudiana Bertoni plants. International Journal of Plant Biology, 15(2), 505-516.
• Jiang, X.W., Zhang, C.R., Wang, W.H., Xu, G.H., & Zhang, H.Y. (2020). Seed priming improves seed germination and seedling growth of Isatis indigotica Fort. under salt stress. HortScience, 55(5), 647-650.
• Kahraman, N.D., & Okumuş, O. (2024). Role of Gibberellic Acid (GA3) in Improving Salt Stress Tolerance of Wheat (Triticum avestivum). Erciyes Tarım ve Hayvan Bilimleri Dergisi, 7(2), 86-93.
• Kan, A. (2011). Investigation of some characteristic of buckwheat (Fagopyrum esculentum Moench) growing in Konya ecological conditions. Selcuk Journal of Agriculture and Food Sciences, 25(4), 67-71.
• Kaur, S., Kumar, K., Singh, L., Sharanagat, V. S., Nema, P. K., Mishra, V., & Bhushan, B. (2024). Gluten-free grains: Importance, processing and its effect on quality of gluten-free products. Critical reviews in food science and nutrition, 64(7), 1988-2015.
• Kodal, A., Sarikamis, G., Sahin, O., & Demir, K. (2025). Salt stress response of cauliflower (Brassica oleracea var. botrytis) seedlings under different LED light wavelengths. International Journal of Agriculture, Environment and Food Sciences, 9 (2): 608-616. https://doi.org/10.31015/2025.2.34
• Mishra, M., & Jain, S. (2019). A comparative study on nutritional profile and antinutrients of buckwheat fractions (Fagopyrum esculentum). International Journal of Current Microbiology and Applied Sciences, 8(3), 3384-3393.
• Mondal, S. (2023). Fagopyrum esculentum: A Nutrient-Dense Part of Nature. Phytochemical Composition and Pharmacy of Medicinal Plants: 2-volume set, 325.
• Munns, R., Rawson, H.M. (1999). Effect of salinity on salt accumulation and reproductive development in the apical meristem of wheat and barley. Functional Plant Biology, 26(5), 459-464.
• Nadeem, M., Shahbaz, M., Ahmad, F., & Waraich, E. A. (2025). Enhancing wheat resistance to salinity: The role of gibberellic acid and β-Carotene in morphological, yielding and ionic adaptations. Journal of Ecological Engineering, 26(6), 76-94.
• Selwal, N., Bedi, M., Hamid, S., & Pujari, M. (2022). Buckwheat (Fagopyrum esculentum) response and tolerance to abiotic stress. In Omics Approach to Manage Abiotic Stress in Cereals (pp. 575-597). Singapore: Springer Nature Singapore.
• Shahzad, K., Hussain, S., Arfan, M., Hussain, S., Waraich, E. A., Zamir, S., Saddique, M., Rauf, A., Kamal, K.Y., Hano, C., & El-Esawi, M. A. (2021). Exogenously applied gibberellic acid enhances growth and salinity stress tolerance of maize through modulating the morpho-physiological, biochemical and molecular attributes. Biomolecules, 11(7), 1005.
• Singh, A. (2022). Soil salinity: A global threat to sustainable development. Soil Use and Management, 38(1), 39-67.
• Singh, M., Malhotra, N., & Sharma, K. (2020). Buckwheat (Fagopyrum sp.) genetic resources: What can they contribute towards nutritional security of changing world?. Genetic Resources and Crop Evolution, 67(7), 1639-1658.
• Snedecor, G. W., & W. G. Cochran. (1967). Statistical Methods, The Iowa State University Press, Ames, Iowa.
• Sofi, S. A., Ahmed, N., Farooq, A., Rafiq, S., Zargar, S. M., Kamran, F., Dar, T.A., Mir, S.A., Dar, B.N., Mousavi Khaneghah, A. (2023). Nutritional and bioactive characteristics of buckwheat, and its potential for developing gluten‐free products: An updated overview. Food Science and Nutrition, 11(5), 2256-2276.
• Sood, R., Singh, V., Chaudhary, S. (2021). Insight into Buckwheat Breeding. Agriculture and Food: E-Newsletter.
• Wang, C.L., Ding, M.Q., Zou, C.Y., Zhu, X.M., Tang, Y., Zhou, M.L., & Shao, J. R. (2017). Comparative analysis of four buckwheat species based on morphology and complete chloroplast genome sequences. Scientific Reports, 7(1), 6514.
• Yadav, S., Irfan, M., Ahmad, A., & Hayat, S. (2011). Causes of salinity and plant manifestations to salt stress: a review. Journal of Environmental Biology, 32(5), 667.
• Yin, C., Jiang, C., Shi, P., Xu, J., Zhai, Y., & Shi, W. (2025). Phytohormone pretreatment mitigates the negative impacts of salt stress on the germination of common buckwheat seeds (Fagopyrum esculentum). New Zealand Journal of Crop and Horticultural Science, 1-12.
• Zhang, K., Khan, M. N., Luo, T., Bi, J., Hu, L., & Luo, L. (2024). Seed priming with gibberellic acid and ethephon improved rice germination under drought stress via reducing oxidative and cellular damage. Journal of Soil Science and Plant Nutrition, 24(2), 2679-2693.