Research Article

Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment

Number: 2026 September 27, 2026
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Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment

Abstract

Background: Rural water sources shared by humans and animals may pose microbiological risks to public health. Aim: This study evaluated physicochemical and microbiological water quality in Kars Province, emphasizing temporal variation, persistence of bacterial indicators, and multivariate patterns. Materials and Methods: Thirty-eight sources were sampled monthly for seven months, yielding 266 site-month records. Physicochemical parameters, Escherichia coli (E. coli), and coliform bacteria were analyzed. Monthly differences were assessed with Friedman and Cochran’s Q tests, location-level associations with Spearman correlation, and multivariate patterns with principal component and hierarchical cluster analyses. Results: E. coli positivity ranged from 57.9% to 71.1%, and positivity for coliform bacteria from 52.6% to 73.7%, with significant monthly differences (p=0.018 and p=0.002, respectively). E. coli and coliform bacteria were detected during at least five months at 27 and 28 locations, respectively. Iron, nitrate, aluminum, pH, conductivity, turbidity, and copper differed significantly across months. No physicochemical parameter correlated significantly with either bacterial indicator, whereas the levels of E. coli and coliform bacteria were strongly correlated (ρ=0.940, p<0.001). The first three principal components explained 61.80% of variance, and clustering identified three location groups. Conclusion: Repeated monitoring demonstrated persistent bacterial-indicator contamination and temporal and site-specific heterogeneity. Because pathogens and microbial sources were not directly assessed, contamination sources could not be established. Persistently positive locations should be prioritized for confirmatory testing, sanitary assessment, source protection, and treatment before human consumption

Keywords

Water quality, Physicochemical parameters, Microbiological contamination, Escherichia coli, Kars (Turkey)

Ethical Statement

Ethical approval for this study was granted by the Scientific Research Ethics Committee of the Institute of Science on March 2, 2026, under Decision No. 8 and Reference No. 18.

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APA
Kalaçay, D., İzkübarlas, K., & Savaş, B. (2026). Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1921785
AMA
1.Kalaçay D, İzkübarlas K, Savaş B. Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment. JAES. 2026;(2026). doi:10.35229/jaes.1921785
Chicago
Kalaçay, Destan, Kenan İzkübarlas, and Bedrettin Savaş. 2026. “Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment”. Journal of Anatolian Environmental and Animal Sciences, nos. 2026. https://doi.org/10.35229/jaes.1921785.
EndNote
Kalaçay D, İzkübarlas K, Savaş B (September 1, 2026) Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment. Journal of Anatolian Environmental and Animal Sciences 2026
IEEE
[1]D. Kalaçay, K. İzkübarlas, and B. Savaş, “Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment”, JAES, no. 2026, Sept. 2026, doi: 10.35229/jaes.1921785.
ISNAD
Kalaçay, Destan - İzkübarlas, Kenan - Savaş, Bedrettin. “Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment”. Journal of Anatolian Environmental and Animal Sciences. 2026 (September 1, 2026). https://doi.org/10.35229/jaes.1921785.
JAMA
1.Kalaçay D, İzkübarlas K, Savaş B. Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment. JAES. 2026. doi:10.35229/jaes.1921785.
MLA
Kalaçay, Destan, et al. “Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment”. Journal of Anatolian Environmental and Animal Sciences, no. 2026, Sept. 2026, doi:10.35229/jaes.1921785.
Vancouver
1.Destan Kalaçay, Kenan İzkübarlas, Bedrettin Savaş. Temporal Variation And Persistence Of Bacterial Water-Quality Indicators In Rural Water Sources Of Kars Province, Türkiye: A Seven-Month Physicochemical And Microbiological Assessment. JAES. 2026 Sep. 1;(2026). doi:10.35229/jaes.1921785