Research Article

Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes

Number: 2026 July 18, 2026
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Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes

Abstract

Palbociclib (Pal), is one of the best-in class oral cyclin-dependent kinase inhibitors (CDKi) used in new generation cancer therapy. Lavandulyl acetate (LA), a monoterpene-derived constituent of lavender essential oil (LEO), is known for its antibacterial, antigenotoxic, and anticancer properties. Although anticancers primarily aims to eliminate cancer cells using anticancer agents, healthy cells are also unintentionally affected during treatment. Therefore, this study aimed to investigate the antigenotoxic and protective potential of LA against Pal-induced DNA damage in healthy human lymphocytes. The alkaline Comet Assay was employed to evaluate: (1) the genotoxic effects of a single dose of Pal (20 µM), (2) the genotoxicity of the highest concentration of LA (LA-H: 0.015 µL/mL), and (3) the antigenotoxic effects of three concentrations of LA administered simultaneously with Pal (LA-H: 0.015 µL/mL, LA-M: 0.007 µL/mL, and LA-L: 0.003 µL/mL). The results demonstrated that Pal induced significant DNA damage, whereas LA-H showed no genotoxic effects. In contrast, LA exhibited a protective effect against Pal-induced genotoxicity. Among the tested concentrations, LA-M displayed the highest antigenotoxic activity, providing protection rates of 114.67%, 125.33%, and 106.93% in tail intensity, tail length, and tail moment, respectively. In conclusion, this study is the first to comprehensively evaluate both the intrinsic genotoxic potential of lavandulyl acetate and its antigenotoxic activity against the anticancer drug Pal, highlighting its possible chemoprotective potential.

Keywords

Alkaline comet assay, antigenotoxicity, genotoxicity, lavandulyl acetate, palbociclib, peripheral human lymphocytes

Supporting Institution

Gazi University Scientific Research Projects Coordination Unit (BAP)

Project Number

FDK-2024-9044

Ethical Statement

This thesis study was carried out with the approval of the Gazi University Faculty of Medicine Clinical Research Ethics Committee (approval date: 21 November 2022; approval number: 854).

Thanks

This study was supported by the Gazi University Scientific Research Projects Coordination Unit (Project No: FDK-2024-9044).

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APA
Duran, İ. F., Ünal, F., & Yüzbaşıoğlu, D. (2026). Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1951594
AMA
1.Duran İF, Ünal F, Yüzbaşıoğlu D. Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes. JAES. 2026;(2026). doi:10.35229/jaes.1951594
Chicago
Duran, İrem Funda, Fatma Ünal, and Deniz Yüzbaşıoğlu. 2026. “Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes”. Journal of Anatolian Environmental and Animal Sciences, nos. 2026. https://doi.org/10.35229/jaes.1951594.
EndNote
Duran İF, Ünal F, Yüzbaşıoğlu D (July 1, 2026) Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes. Journal of Anatolian Environmental and Animal Sciences 2026
IEEE
[1]İ. F. Duran, F. Ünal, and D. Yüzbaşıoğlu, “Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes”, JAES, no. 2026, July 2026, doi: 10.35229/jaes.1951594.
ISNAD
Duran, İrem Funda - Ünal, Fatma - Yüzbaşıoğlu, Deniz. “Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes”. Journal of Anatolian Environmental and Animal Sciences. 2026 (July 1, 2026). https://doi.org/10.35229/jaes.1951594.
JAMA
1.Duran İF, Ünal F, Yüzbaşıoğlu D. Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes. JAES. 2026. doi:10.35229/jaes.1951594.
MLA
Duran, İrem Funda, et al. “Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes”. Journal of Anatolian Environmental and Animal Sciences, no. 2026, July 2026, doi:10.35229/jaes.1951594.
Vancouver
1.İrem Funda Duran, Fatma Ünal, Deniz Yüzbaşıoğlu. Protective Potential of Lavandulyl Acetate Against Palbociclib-Induced DNA Damage: A Comet Assay Analysis in Human Lymphocytes. JAES. 2026 Jul. 1;(2026). doi:10.35229/jaes.1951594