Research Article

Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans

Number: 2026 September 29, 2026
EN TR

Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans

Abstract

Serratia proteamaculans is an environmentally widespread member of the genus Serratia isolated from aquatic animals, food products, plants, insects, environmental habitats and clinical sources. Ecologically broadly distributed, little is known about its genomic diversity and factors associated with antimicrobial resistance and virulence. The present study reports a comparative genomic analysis of 38 publicly available S. proteamaculans genomes to explore genomic diversity, antimicrobial resistance determinants, integrons, and bacterial secretion systems. Genome sizes were 4.99–5.93 Mb with GC contents of 54.5–55.5%. Pairwise average nucleotide identity (ANI) values of 97.0-99.9% confirmed that all genomes belonged to the same species, while rpoB-based phylogenetic analysis revealed moderate intraspecies diversity. None of the genomes had any integrons. Comparative resistome analysis identified 260 predicted antimicrobial resistance elements, including a conserved core resistome mainly composed of multidrug efflux pumps, aminoglycoside-modifying enzymes, regulatory proteins and chromosomally encoded β-lactamase-associated genes, and a variable accessory resistome likely acquired through horizontal gene transfer. Analysis of secretion systems revealed that the Type I secretion system (T1SS), Type IVa pili (T4aP), Type V secretion system (T5SS), and the flagellar export apparatus were present in all genomes, while the Type II secretion system (T2SS), Type VI secretion system (T6SS), Type IV secretion systems (T4SSs), Type IVb pili (T4bP), and Tight adherence (Tad) systems were present in a variable manner. These results suggest a conserved genomic backbone with accessory traits which are variable and probably responsible for ecological adaptation, environmental persistence and strain-specific functional diversity in S. proteamaculans.

Keywords

Antimicrobial resistance, comparative genomics, secretion systems, Serratia proteamaculans, virulence factors

References

  1. Abby, S. S., Cury, J., Guglielmini, J., Neron, B., Touchon, M., & Rocha, E. P. (2016). Identification of protein secretion systems in bacterial genomes. Sci Rep, 6, 23080. https://doi.org/10.1038/srep23080
  2. Abby, S.S., Denise, R., & Rocha, E.P.C. (2024). Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder Version 2. Methods Mol Biol, 2715, 1-25. https://doi.org/10.1007/978-1-0716-3445-5_1
  3. Abby, S.S., & Rocha, E.P.C. (2017). Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder. Methods Mol Biol, 1615, 1- 21. https://doi.org/10.1007/978-1-4939-7033-9_1
  4. Alcock, B. P., Huynh, W., Chalil, R., Smith, K. W., Raphenya, A. R., Wlodarski, M. A., Edalatmand, A., Petkau, A., Syed, S. A., Tsang, K. K., Baker, S. J. C., Dave, M., McCarthy, M. C., Mukiri, K. M., Nasir, J. A., Golbon, B., Imtiaz, H., Jiang, X., Kaur, K., Kwong, M., Liang, Z. C., Niu, K. C., Shan, P., Yang, J. Y. J., Gray, K. L., Hoad, G. R., Jia, B., Bhando, T., Carfrae, L. A., Farha, M. A., French, S., Gordzevich, R., Rachwalski, K., Tu, M. M., Bordeleau, E., Dooley, D., Griffiths, E., Zubyk, H. L., Brown, E. D., Maguire, F., Beiko, R. G., Hsiao, W. W. L., Brinkman, F. S. L., Van Domselaar, G., & McArthur, A. G. (2023). CARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database. Nucleic Acids Res, 51(D1), D690-D699. https://doi.org/10.1093/nar/gkac920
  5. Angiuoli, S. V., Gussman, A., Klimke, W., Cochrane, G., Field, D., Garrity, G., Kodira, C. D., Kyrpides, N., Madupu, R., Markowitz, V., Tatusova, T., Thomson, N., & White, O. (2008). Toward an online repository of Standard Operating Procedures (SOPs) for (meta)genomic annotation. OMICS, 12(2), 137-141. https://doi.org/10.1089/omi.2008.0017
  6. Austin, B., & Austin, D.A. (2016). Bacterial Fish Pathogens: Disease of Farmed and Wild Fish (pp. 1 online resource (XXXV, 732 pages 748 illustrations in color). https://doi.org/10.1007/978-3-319-32674-0
  7. Begrem, S., Jerome, M., Leroi, F., Delbarre-Ladrat, C., Grovel, O., & Passerini, D. (2021). Genomic diversity of Serratia proteamaculans and Serratia liquefaciens predominant in seafood products and spoilage potential analyses. Int J Food Microbiol, 354, 109326. https://doi.org/10.1016/j.ijfoodmicro.2021.10932 6
  8. Bernardet, J.F., & Bowman, J.P. (2015). Bergey’s Manual of Systematics of Archaea and Bacteria. https://doi.org/10.1002/9781118960608.gbm003 12
  9. Blair, J.M., Webber, M.A., Baylay, A.J., Ogbolu, D.O., & Piddock, L.J. (2015). Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol, 13(1), 42-51. https://doi.org/10.1038/nrmicro3380
  10. Cabello, F.C., Godfrey, H.P., Buschmann, A.H., & Dolz, H.J. (2016). Aquaculture as yet another environmental gateway to the development and globalisation of antimicrobial resistance. Lancet Infect Dis, 16(7), e127-e133. https://doi.org/10.1016/S1473-3099(16)00100-6
APA
Kumru, S. (2026). Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1992369
AMA
1.Kumru S. Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans. JAES. 2026;(2026). doi:10.35229/jaes.1992369
Chicago
Kumru, Salih. 2026. “Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia Proteamaculans”. Journal of Anatolian Environmental and Animal Sciences, nos. 2026. https://doi.org/10.35229/jaes.1992369.
EndNote
Kumru S (September 1, 2026) Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans. Journal of Anatolian Environmental and Animal Sciences 2026
IEEE
[1]S. Kumru, “Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans”, JAES, no. 2026, Sept. 2026, doi: 10.35229/jaes.1992369.
ISNAD
Kumru, Salih. “Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia Proteamaculans”. Journal of Anatolian Environmental and Animal Sciences. 2026 (September 1, 2026). https://doi.org/10.35229/jaes.1992369.
JAMA
1.Kumru S. Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans. JAES. 2026. doi:10.35229/jaes.1992369.
MLA
Kumru, Salih. “Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia Proteamaculans”. Journal of Anatolian Environmental and Animal Sciences, no. 2026, Sept. 2026, doi:10.35229/jaes.1992369.
Vancouver
1.Salih Kumru. Comparative Genomic Analysis Exposes Conserved Antimicrobial Resistance Elements and Diverse Secretion Systems in Serratia proteamaculans. JAES. 2026 Sep. 1;(2026). doi:10.35229/jaes.1992369