6-SHOGAOL, 6-GİNGEROL VE KURKUMİN’ İN A549 HÜCRELERİNDE CANLILIK ÜZERİNE ETKİLERİ

Yıl 2017, Cilt: 26 Sayı: 1, 24 - 28, 01.03.2017

Eren Demirpolat Mükerrem Betül Yerer Aycan

Öz

Çalışmamızda A549 küçük hücreli olmayan akciğer kanseri hücrelerinde Zingiber officinale’de bulunan 6- gingerol ile 6-shogaolün antikanser etkileri canlılık üzerinden araştırılmıştır. Deneyler hem IL-1β’ ya maruz
kalan hem de maruz kalmayan hücrelerde yürütüldü.
Curcuma longa’ da bulunan kurkumin bilinen doğal bir
antikanser molekül olduğundan çalışmada pozitif kontrol olarak kullanıldı ve kurkumine yapıca benzer olan 6
-gingerol ile 6-shogaolün canlılık üzerine olan etkileri
kurkuminle kıyaslandı. MTT (3-(4,5-dimetiltiazol-2-il)- 2,5-difenil tetrazolyum bromür) deneyleri öncelikle 6- gingerol ve 6-shogaolün birbirini logaritmik şekilde
seyreden konsantrasyonlarında yapıldı. Daha sonra etki
görülen konsantrasyon aralığını kapsayacak şekilde IC50 değerini belirlemek için deneyler tekrarlandı. Sonuç
olarak 6-shogaolun 24 sa IC50 değeri IL-β’ ya maruz kalmayan hücrelerde 62,5 μM; IL-β’ ya maruz kalan hücrelerde ise 63,2 μM olarak bulundu. Kurkumin 100 μM
konsantrasyonu ile kıyaslandığında 6-shogaolün daha
önceden antikanser özelliği gösterilmiş olan kurkumin
kadar etkin olduğu görülürken; 6-gingerol için canlılığı
azaltıcı yönde bir bulguya rastlanmadı. 6-shogaol’ un
canlılığı azaltıcı etkisinin altında yatan moleküler
mekanizmalar araştırılmaya değerdir.

Anahtar Kelimeler

A549, 6-gingerol, 6-shogaol, kurkumin, IL-1β

Effects of 6-Shogaol, 6-Gingerol and Curcumin on Cell Viability in A549 Cancer Cell Line

Öz

In our study we investigated anticancer effects of 6- gingerol and 6-shogaol from Zingiber officinale. Anticancer effect was monitored by using cell viability assay.
Experiments are performed on both A549 cells that are
incubated with IL-1β (interleukin 1 β) and cells which
are not incubated with it. Curcumin from Curcuma
longa, a natural compound that is known for its anticancer effect, is used as a positive control and effects of 6- gingerol and 6-shogaol are compared with it. MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) assay was performed for wide range concentrations. Concentration changes were 10 fold. To obtain
IC50 values of 6-shogol and 6-gingerol, MTT assays were
performed again, using the previous concentrations but
in a narrower range.According to the assays 6-shogaol’s
24 h IC50 determined as 62,5 μM for A549 cells which
were not incubated with IL-1β and 63,2 μM for the cells
which were exposed to IL-1β. 6-shogaol’s cytotoxic effect was as much as curcumin which is a known natural
anticancer molecule, while 6-gingerol did not show any
effect towards decreasing the cell viability. Mechanisms
which underly 6-shogoal’s cytotoxic effect are worth for
further investigation.

Anahtar Kelimeler

A549, 6-gingerol, 6-shogaol, curcumin, IL- 1β

Kaynakça

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