BibTex RIS Kaynak Göster

Sperm fonksiyon testlerinin in vitro fertilizasyon uygulamalarında fertilizasyon başarısızlığına etkisi

Yıl 2002, Cilt: 33 Sayı: 3, 12 - 14, 01.04.2002

Cem Fıçıcıoğlu Tülay Irez Ayça Çubukçu Serap Yaltı Birgül Gürbüz Ebru Çöğendez

Öz

Objective:To investigatethe effects of sperm function test on IVF failure. Methods: In this study spontaneous and the stimulated acrosome reaction (AR) and Hypoosmotic swelling test (HOS) was performed for normospermic samples. Ca ionophore was A23187 used for maximal acrosome loss and hypoosmotic media (Fructose,Na cit-rate 150 mOsm) for swelling of spermatozoa, respec-tively. Results: We found that induced AR results had higher fertilization success than spontaneous AR results and a significant correlation between HOS grade 1-4 and fertilization rates. Conclusion: Stimulated AR test and HOS test grades can predict sperm fertilization ability in IVF.

Anahtar Kelimeler

Fertilization in Vitro Spermatozoa Acrosome Reaction Treatment Failure

Kaynakça

  • l.BuchJ.P. Advanced sperm function testing. Current opinion in urology, 1993, 3, 503-508 2. Zeneveld J.D.L. and Jeyendran R.S. Sperm function tests
  • Infertility and Reproductive Medicine, Clinics of North America , Eds. M.P.Diamond and A.H.De Cherney, W.B.Sounders Co, PA, 1992, p.353-372 3. Mortimer D., Curtis E.F., Camezind A.R. and Tanaka S. The spontaneous acrosome reaction of human spermatozoa incubated in vitro. Human Reproduction, 1989 4,1,57-62 4. Plachot M., Mandelbaum ]., Junca A.M. Acrosome reaction of human sperm used for in vitro fertilization. Fertti Steril, 1984, 42; 418-420
  • 5. Mortimer D. and Camenzind A.R. The role of follicular fluid in inducing the acrosome reaction of human spermatozoa incubated in vitro. Human Reproduction, 1989, 4,2; 169-174 6. Jeyendran R.S., Van der Ven H.H.,Palez M. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
  • J.Reproduct. Fertil 1984,70:219-228 7. Plymate S.R., Nagao R.R., Muller C.H., Paulsen CA.) The use of sperm penetration assay in evaluation of men with varicocele
  • Fertil Steril, 1987, 47,4,680-683 8. Mordel N.,Mor Yosef S., Margalioth E.J., Shemesh A., Samuelof A., SchenkerJ.G. The human sperm hypoosmotic swelling test: Its practical application and suggestion for improvement. IntJ.Fertil, 1989, 34:355-358 9. Mordel N.,Dano I.,Epistein Eldan M.,Shemesh A.,Schenker J.G.,Laufer N. Novel parameters of human sperm hypoosmotic swelling test and their correlation to standard spermatogrdm, total motile sperm fraction and sperm penetration assay. Fertil Steril, 1993 59,6,1276-1279 10. Chan S.Y.,Craft L.L.,Chan Y.M.,Leung C.K. The hypoosmotic swelling test and cryosurvival of human spermatozoa. Human Reproduction, 1990 5,715-718 11. Chan S.Y.W.,Fox EJ.,Chan M.M.C.Jsoi W.,Wang C.Tang C.C. Multivariate discriminant analysis of the relationship between the hypoosmotic swelling test and the invitro fertilizing capacity of human sperm. Int.J. Androl. (1988,11,369-378 12. Yanagimachi R. Mammalian fertilization. InThe physiology of reproduction(Eds,E.Knobil,J,Neill,L.L.Eving,g.s.Greenvald, C.L.Markert andD.W.Pfaff), 1988, pp. 135-185 (Raven Press New York) 13. Fenichel P.,Donzeau M.,Farahifar D.,Basteris B.,Ayraund N.,Li His B. Dynamics of sperm acrosome reaction, relation with in vitro fertilization. Fertil Steril, 1991, 55,5,994-999

The relation of sperm function tests and fertilization failure in invitro fertilization

Yıl 2002, Cilt: 33 Sayı: 3, 12 - 14, 01.04.2002

Cem Fıçıcıoğlu Tülay Irez Ayça Çubukçu Serap Yaltı Birgül Gürbüz Ebru Çöğendez

Öz

Amaç: Sperm fonksiyon testlerinin fertilizasyon başarısızlığına etkisi Metod: Spontan ve stimule akrosom reaksiyon (AK) and Hypoosmotik şişme testi (HOS) normospermik örneklerde uygulandı. Ca ionophore, A23187 maksimal acrosome kaybı ve hypoosmotic medyum (Fructose, Na citrate 150 mOsm) spermatozoa'yı şişirmek için sırası ile kullanıldı. Bulgular: İndüklenmiş AR, spontan AR sonuçlarına göre daha yüksek fertilizasyon başarı gösterdi. HOS grade l-4 ile fertilizasyon oranlan arasında anlamlı ilişki bulundu Sonuç: Stimulate AR testi ve HOS test dereceleri IVF'de fertilizasyon olup olmayacağını önceden tahmin etmede önemli role sahiptir.

Anahtar Kelimeler

İnvitro fertilizasyon Sperm Akrosom reaksiyonu Tedavi başarısızlığı

Kaynakça

  • l.BuchJ.P. Advanced sperm function testing. Current opinion in urology, 1993, 3, 503-508 2. Zeneveld J.D.L. and Jeyendran R.S. Sperm function tests
  • Infertility and Reproductive Medicine, Clinics of North America , Eds. M.P.Diamond and A.H.De Cherney, W.B.Sounders Co, PA, 1992, p.353-372 3. Mortimer D., Curtis E.F., Camezind A.R. and Tanaka S. The spontaneous acrosome reaction of human spermatozoa incubated in vitro. Human Reproduction, 1989 4,1,57-62 4. Plachot M., Mandelbaum ]., Junca A.M. Acrosome reaction of human sperm used for in vitro fertilization. Fertti Steril, 1984, 42; 418-420
  • 5. Mortimer D. and Camenzind A.R. The role of follicular fluid in inducing the acrosome reaction of human spermatozoa incubated in vitro. Human Reproduction, 1989, 4,2; 169-174 6. Jeyendran R.S., Van der Ven H.H.,Palez M. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
  • J.Reproduct. Fertil 1984,70:219-228 7. Plymate S.R., Nagao R.R., Muller C.H., Paulsen CA.) The use of sperm penetration assay in evaluation of men with varicocele
  • Fertil Steril, 1987, 47,4,680-683 8. Mordel N.,Mor Yosef S., Margalioth E.J., Shemesh A., Samuelof A., SchenkerJ.G. The human sperm hypoosmotic swelling test: Its practical application and suggestion for improvement. IntJ.Fertil, 1989, 34:355-358 9. Mordel N.,Dano I.,Epistein Eldan M.,Shemesh A.,Schenker J.G.,Laufer N. Novel parameters of human sperm hypoosmotic swelling test and their correlation to standard spermatogrdm, total motile sperm fraction and sperm penetration assay. Fertil Steril, 1993 59,6,1276-1279 10. Chan S.Y.,Craft L.L.,Chan Y.M.,Leung C.K. The hypoosmotic swelling test and cryosurvival of human spermatozoa. Human Reproduction, 1990 5,715-718 11. Chan S.Y.W.,Fox EJ.,Chan M.M.C.Jsoi W.,Wang C.Tang C.C. Multivariate discriminant analysis of the relationship between the hypoosmotic swelling test and the invitro fertilizing capacity of human sperm. Int.J. Androl. (1988,11,369-378 12. Yanagimachi R. Mammalian fertilization. InThe physiology of reproduction(Eds,E.Knobil,J,Neill,L.L.Eving,g.s.Greenvald, C.L.Markert andD.W.Pfaff), 1988, pp. 135-185 (Raven Press New York) 13. Fenichel P.,Donzeau M.,Farahifar D.,Basteris B.,Ayraund N.,Li His B. Dynamics of sperm acrosome reaction, relation with in vitro fertilization. Fertil Steril, 1991, 55,5,994-999
Toplam 5 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Cem Fıçıcıoğlu Bu kişi benim

Tülay Irez Bu kişi benim

Ayça Çubukçu Bu kişi benim

Serap Yaltı Bu kişi benim

Birgül Gürbüz Bu kişi benim

Ebru Çöğendez Bu kişi benim

Yayımlanma Tarihi 1 Nisan 2002
Yayımlandığı Sayı Yıl 2002 Cilt: 33 Sayı: 3

Kaynak Göster

APA Fıçıcıoğlu, C., Irez, T., Çubukçu, A., Yaltı, S., vd. (2002). The relation of sperm function tests and fertilization failure in invitro fertilization. Zeynep Kamil Tıp Bülteni, 33(3), 12-14. https://doi.org/10.16948/zktb.95955
AMA Fıçıcıoğlu C, Irez T, Çubukçu A, Yaltı S, Gürbüz B, Çöğendez E. The relation of sperm function tests and fertilization failure in invitro fertilization. Zeynep Kamil Tıp Bülteni. Nisan 2002;33(3):12-14. doi:10.16948/zktb.95955
Chicago Fıçıcıoğlu, Cem, Tülay Irez, Ayça Çubukçu, Serap Yaltı, Birgül Gürbüz, ve Ebru Çöğendez. “The Relation of Sperm Function Tests and Fertilization Failure in Invitro Fertilization”. Zeynep Kamil Tıp Bülteni 33, sy. 3 (Nisan 2002): 12-14. https://doi.org/10.16948/zktb.95955.
EndNote Fıçıcıoğlu C, Irez T, Çubukçu A, Yaltı S, Gürbüz B, Çöğendez E (01 Nisan 2002) The relation of sperm function tests and fertilization failure in invitro fertilization. Zeynep Kamil Tıp Bülteni 33 3 12–14.
IEEE C. Fıçıcıoğlu, T. Irez, A. Çubukçu, S. Yaltı, B. Gürbüz, ve E. Çöğendez, “The relation of sperm function tests and fertilization failure in invitro fertilization”, Zeynep Kamil Tıp Bülteni, c. 33, sy. 3, ss. 12–14, 2002, doi: 10.16948/zktb.95955.
ISNAD Fıçıcıoğlu, Cem vd. “The Relation of Sperm Function Tests and Fertilization Failure in Invitro Fertilization”. Zeynep Kamil Tıp Bülteni 33/3 (Nisan 2002), 12-14. https://doi.org/10.16948/zktb.95955.
JAMA Fıçıcıoğlu C, Irez T, Çubukçu A, Yaltı S, Gürbüz B, Çöğendez E. The relation of sperm function tests and fertilization failure in invitro fertilization. Zeynep Kamil Tıp Bülteni. 2002;33:12–14.
MLA Fıçıcıoğlu, Cem vd. “The Relation of Sperm Function Tests and Fertilization Failure in Invitro Fertilization”. Zeynep Kamil Tıp Bülteni, c. 33, sy. 3, 2002, ss. 12-14, doi:10.16948/zktb.95955.
Vancouver Fıçıcıoğlu C, Irez T, Çubukçu A, Yaltı S, Gürbüz B, Çöğendez E. The relation of sperm function tests and fertilization failure in invitro fertilization. Zeynep Kamil Tıp Bülteni. 2002;33(3):12-4.