Two different methods for rooting blackberry cuttings: comparison of aeroponic and perlite media

Year 2025, Volume: 9 Issue: 1, 190 - 198, 17.03.2025

Hakan Karadağ , Fatma Dumlu

https://doi.org/10.31015/2025.1.21

Abstract

This study examines the effects of different IBA concentrations and rooting media on the rooting characteristics of green cuttings from the Jumbo blackberry variety. The cuttings were treated with 0 (control), 500, 1000, and 1500 ppm IBA, then planted in Aeroponic and Perlite rooting systems to compare their performance. Parameters such as rooting rate, root length, development of rootlets, seedling yield, the number of branches per cutting, and disease occurrence were evaluated.
In the Perlite medium, the application of 1500 ppm resulted in the most extended root length (7.33 cm), while the highest root number (13.26) was observed at the 500 ppm dose. In the Aeroponic medium, the 1000 ppm application achieved the highest values for root length (10.24 cm) and root number (15.47). However, while the decay rate remained at 0.00% in the Perlite medium, it varied between 16.67% and 20.00% in the Aeroponic medium. The highest rooting rate in the Perlite medium was observed at 500 ppm with 93.33%, whereas in the Aeroponic medium, the rooting rate ranged from 50.00% in the control group to a maximum of 70.00% in the 1500 ppm group. The seedling yield reached 93.33% at 500 ppm in the Perlite medium and peaked at 63.33% in the Aeroponic medium.
In conclusion, the Perlite medium provided healthier root development due to its low decay rate, higher callus formation, and higher rooting rates. In contrast, the Aeroponic medium posed a risk of decay even at higher IBA concentrations.

Keywords

Rooting , IBA , Blackberry , Jumbo , Green cuttings

References

• Akbulut, M., Kaplan, N., Macit, İ., & Özdemir, C. (2003). Samsun Çarşamba Ovası Koşullarına uygun böğürtlen çeşitlerinin belirlenmesi. Ulusal Kivi ve Üzümsü Meyveler Sempozyumu Bildirileri, 357-360. (in Turkish)
• Balliu, A., Zheng, Y., Sallaku, G., Fernández, J. A., Gruda, N. S., & Tuzel, Y. (2021). Environmental and Cultivation Factors Affect the Morphology, Architecture and Performance of Root Systems in Soilless Grown Plants. Horticulturae, 7(8), 243. https://doi.org/10.3390/horticulturae7080243
• Baiyin, B., Tagawa, K., Yamada, M., Wang, X., Yamada, S., Shao, Y., An, P., Yamamoto, S., & Ibaraki, Y. (2021). Effect of Nutrient Solution Flow Rate on Hydroponic Plant Growth and Root Morphology. Plants, 10(9), 1840. https://doi.org/10.3390/plants10091840
• Barrett, G. E., Alexander, P. D., Robinson, J. S., & Bragg, N. C. (2016). Achieving environmentally sustainable growing media for soilless plant cultivation systems–A review. Scientia horticulturae, 212, 220-234. https://doi.org/10.1016/j.scienta.2016.09.030
• Benke, K., & Tomkins, B. (2017). Future food-production systems: vertical farming and controlled-environment agriculture. Sustainability: science, practice and policy, 13(1), 13-26. https://doi.org/10.1080/15487733.2017.1394054
• Bingöl, B. 2019. Alternatif Tarım Yöntemleri; Aeroponik, Akuaponik, Hidroponik.Harman Time Dergisi, Aralık/2019, 7(82), 34-42, ISSN: 2147-6004. (in Turkish)
• Cai, J., Veerappan, V., Arildsen, K. et al. A modified aeroponic system for growing small-seeded legumes and other plants to study root systems. Plant Methods 19, 21 (2023). https://doi.org/10.1186/s13007-023-01000-6
• Chen, J., & Stamps, R. H. (2006). Cutting propagation of foliage plants. Cutting propagation: A guide to propagating and producing floriculture crops. Ball Publishing, Batavia, IL, 203-228.
• Eldridge, B. M., Manzoni, L. R., Graham, C. A., Rodgers, B., Farmer, J. R., & Dodd, A. N. (2020). Getting to the roots of aeroponic indoor farming. New Phytologist, 228(4), 1183-1192. https://doi.org/10.1111/nph.16780
• Eryılmaz, G. A., Kılıç, O. (2018). Türkiye’de sürdürülebilir tarım ve iyi tarım uygulamaları. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(4), 624-631. (in Turkish)
• Food and Agriculture Organization of the United Nations. (2017). The future of food and agriculture: Trends and challenges. Fao.
• Gerçekcioğlu, R., 2009. Genel Meyvecilik. R. Gerçekcioğlu, Ş. Bilginer ve A. Soylu (Eds.), Çeliklerde Kök Oluşumu, Nobel Yayınları, s. 247–250. (in Turkish)
• Gislerod HR, Mortensen LM. 1990. Relative humidity and nutrient con-centration affect nutrient uptake and growth of Begonia. Hortsci. 25(5):524–526.
• Gül, A., Eşiyok, D., & Anac, D. (2010). Topraksız Tarım: Temel İlkeler ve Uygulamalar. Meta Basım Yayın. (in Turkish)
• Gregory, J. G., Brian K. M., William A. J. (1999). Comparison of Perlite and Peat:perlite Rooting Media for Rooting Softwood Stem Cuttings in a Subirrigation System with Minimal Mist. Journal of Environmental Horticulture (1999) 17 (3): 147–151. https://doi.org/10.24266/0738-2898-17.3.147
• Grillas, S., Lucas, M., Bardopoulou, E., Sarafopoulos, S. & Voulgari, M. (2001). Perlite Based Soilless Culture Systems: Current Commercial Applications And Prospects. Acta Hortic. 548, 105-114 DOI: 10.17660/ActaHortic.2001.548.10
• Handreck, K. A., Black, N. (2002). Growing Media for Ornamental Plants and Turf. UNSW Press.
• Hayden, A.L., Yokelsen, T.N., Giacomelli, G.A. & Hoffmann, J.J. (2004). Aeroponics: An Alternative Production System For High-Value Root Crops. Acta Hortic. 629, 207-213 DOI: 10.17660/ActaHortic.2004.629.27
• Kalyoncu, İ. H., 1996. Konya Yöresindeki Kızılcık (Cornus mas L.) Tiplerinin Bazı Özellikleri ve Farklı Nem Ortamlarındaki Köklenme Durumu Üzerine Bir Araştırma, Selçuk Üniv. Tarımsal Yapılar ve Sulama Anabilim Dalı, Doktora Tezi (Basılmamış), Konya. (in Turkish)
• Kalyoncu, H.I., Ersoy, N., Alparslan, F., 2016. Ada Çayı (Salvia Officinalis L.)’Nın Yeşil Çelikle Çoğaltılması Üzerine Farklı Nem Ve Hormon Doz Uygulamalarının Etkileri. Selçuk Tarım Bilimleri Dergisi, 3(2): 171- 176. (in Turkish)
• Kir, A. (2025). Plant Residue-Based Compost Can Replace Peat In Growing Media For Organically Grown Olive Tree Saplings In Mediterranean Climates. Biological Agriculture & Horticulture, 1–18. https://doi.org/10.1080/01448765.2024.2448445
• Mamatha, P., Rao, A. M., Prasanth, P., & Reddy, S. N. (2024). Influence of Different Growing Media on Growth and Flowering of Chrysanthemum (Dendranthema grandiflora) Cultivars for Potmums. International Journal of Environment and Climate Change, 14(11), 888–898. https://doi.org/10.9734/ijecc/2024/v14i114595
• Kratsch HA, Graves WR, Gladon RJ. (2006). Aeroponic system for control of root-zone atmosphere. Environmental and Experimental Botany 55: 70–76. https://doi.org/10.1016/j.envexpbot.2004.10.002
• Kroin, J. (2009). Propagation of plants from cuttings using rooting solutions by foliar methods. In Comb. Proc. Int. Plant Propag. Soc (Vol. 59, pp. 437-453).
• Lakhiar IA, Gao J, Syed TN, Chandio FA, Buttar NA. 2018. Modern plant cultivation technologies in agriculture under controlled environment: a review on aeroponics. Journal of Plant Interactions 13: 338–352. https://doi.org/10.1080/17429145.2018.1472308
• Marschner, H. (2012). Mineral Nutrition of Higher Plants. Academic Press.
• Megersa, H. G. (2017). Propagation methods of selected horticultural crops by specialized organs: review. J Hortic, 4(198), 2376-0354. doi: 10.4172/2376-0354.1000198
• Mehandru, P., Shekhawat, N. S., Rai, M. K., Kataria, V., & Gehlot, H. S. (2014). Evaluation of aeroponics for clonal propagation of Caralluma edulis, Leptadenia reticulata and Tylophora indica–three threatened medicinal Asclepiads. Physiology and Molecular Biology of Plants, 20, 365-373. https://doi.org/10.1007/s12298-014-0240-8
• Pineda-Pineda, J., Roblero, M. d. J. M., León, M. T. C., & Sahagún‐Castellanos, J. (2020). El oxígeno en la zona radical y su efecto en las plantas. Revista Mexicana De Ciencias Agrícolas, 11(4), 931-943. https://doi.org/10.29312/remexca.v11i4.2128
• Preece, J. E., (2003). ACentury of Progress with Vegetative Plant Propagation. Hortscience, Vol. 38(5)
• Ritter E, Angulo B, Riga P, Herrán C, Relloso J, San Jose M. 2001. Comparison of hydroponic and aeroponic cultivation systems for the production of potato minitubers. Potato Research 44: 127–135.
• Scaltrito, E., Cristiano, G., Sdao, A. E., Gruda, N. S., Loconsole, D., & Lucia, B. D. (2024). Influence of water spraying intervals and indole-3-butyric acid concentrations on salvia rooted cuttings quality in a closed aeroponics system. Scientia Horticulturae, 337, 113452. https://doi.org/10.1016/j.scienta.2024.113452
• Schmilewski, G. (2008). The role of peat in assuring the quality of growing media. Mires and peat, 3, 02-8.
• Selvaraj MG, Montoya-P ME, Atanbori J, French AP, Pridmore T. 2019. A low-cost aeroponic phenotyping system for storage root development: unravelling the below-ground secrets of cassava (Manihot esculenta). Plant Methods 15: 131. https://doi.org/10.1186/s13007-019-0517-6
• Sengel, E., Iscı, B., & Altındıslı, A. (2012). Effects of Different Culture Media on Rooting in Grafted Grapevine. Journal of Agriculture Faculty of Ege University, 49(2), 143-148.
• Sharma, U., Kataria, V. & Shekhawat, N.S. Aeroponics for adventitious rhizogenesis in evergreen haloxeric tree Tamarix aphylla (L.) Karst.: influence of exogenous auxins and cutting type. Physiol Mol Biol Plants 24, 167–174 (2018). https://doi.org/10.1007/s12298-017-0493-0
• Yavuz, K., Toksöz, O., Berber, D. (2023). Topraksız tarım teknolojileri gelecek için sürdürülebilir bir çözüm mü?. Frontiers in Life Sciences and Related Technologies, 4(3), 157-170. (in Turkish)