Effects of Propolis Extraction with Different Solvents on Total Phenolic Content, Antioxidant Capacity and Antimicrobial Activity

Yıl 2019, Cilt: 17 Sayı: 4, 538 - 545, 31.12.2019

Zeynep Bakkaloğlu , Muhammet Arıcı

https://doi.org/10.24323/akademik-gida.667272

Cited By: 17

Öz

Propolis, a natural biological product obtained from beekeeping, attracts attention in many aspects including antibacterial, antifungal, antiviral, antitumoral and anesthetic activity. It is also used as a raw material in health, food and cosmetic industries because of its low toxicity. Different solvents are preferred for the extraction of propolis depending on the intended use of the sectors. The different solvents may have an effect on the functional properties of propolis. Extraction of propolis with different solvents may result in differences in the total phenolic content, antioxidant capacity and antimicrobial properties. In this review, different solvents used in propolis extracts and their effects on the total phenolic content, antioxidant capacity and antimicrobial properties of propolis are discussed.

Anahtar Kelimeler

Propolis, Phenolic content, Antioxidant capacity, Antimicrobial activity

Farklı Çözücülerle Propolis Ekstraksiyonunun Toplam Fenolik İçeriği, Antioksidan Kapasite ve Antimikrobiyal Aktivite Üzerine Etkileri

Öz

Arıcılıktan elde edilen doğal bir biyolojik ürün olan propolis antibakteriyel, antifungal, antiviral, antitümoral ve anestezik aktivite dahil olmak üzere birçok yönüyle dikkat çekmektedir. Ayrıca çok düşük toksisiteye sahip olması nedeniyle sağlık, gıda, kozmetik gibi sanayi dallarında hammadde olarak kullanılmaktadır. Sektörlerin kullanım amacına bağlı olarak ekstrakte edilmesi gereken propolis için farklı çözücüler tercih edilmektedir. Kullanılan çözücülerin farklı olması propolisin fonksiyonel özellikleri üzerine etki etmektedir. Propolisin farklı çözücülerle ekstraksiyonu toplam fenolik bileşen içeriği, antioksidan kapasitesi ve antimikrobiyal özelliklerinde farklılıklara neden olmaktadır. Bu derlemede, propolis ekstraktlarında kullanılan farklı çözücüler ve bu çözücülerin propolisin toplam fenolik bileşen, antioksidan kapasitesi ve antimikrobiyal üzerine etkileri tartışılmıştır.

Anahtar Kelimeler

Propolis, Fenolik içerik, Antioksidan kapasitesi, Antimikrobiyal aktivite

Kaynakça

• [1] Şahinler, N. (2000). Arı ürünleri ve insan sağlığı açısından önemi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 5(1-2), 139-148.
• [2] Sangeeta, Kumar, N.R., Virdi, J.K. (2018). Role of propolis in attenuating arsenic toxicity in rat testes. International Journal for Science and Advance Research in Technology, 4(1), 820-824.
• [3] Atik, A., Gümüş, T. (2017). Propolisin gıda endüstrisinde kullanım olanakları. Akademik Gıda, 15(1), 60-65.
• [4] Kasiotis, K.M., Anastasiadou, P., Papadopoulos, A., Machera, K. (2017). Revisiting Greek propolis: chromatographic analysis and antioxidant activity study. PloS one, 12(1), e0170077.
• [5] Viuda-Martos M., Ruiz-Navajas Y., Fernandez-Lopez J., Perez-Alvarez J.A. (2008). Functional properties of honey, propolis and royal jelly. Journal of Food Science, 73(9), 117-124.
• [6] Bankova, V., Decastro, S.L., Marcucci, M.C. (2001). Propolis: recent advances in chemistry and plant origin. Apidologie, 31, 3–15.
• [7] Noureddine, H., Hage-Sleiman, R., Wehbi, B., Fayyad-Kazan, A.H., Hayar, S., Traboulssi, M., ElMakhour, Y. (2017). Chemical characterization and cytotoxic activity evaluation of Lebanese propolis. Biomedicine & Pharmacotherapy, 95, 298-307.
• [8] Kasote, D.M., Pawar, M.V., Bhatia, R.S., Nandre, V.S., Gundu, S.S., Jagtap, S.D., Kulkarni, M.V. (2017). HPLC, NMR based chemical profiling and biological characterisation of Indian propolis. Fitoterapia, 122, 52-60.
• [9] Graikou, K., Popova, M., Gortzi, O., Bankova, V., Chinou, I. (2016). Characterization and biological evaluation of selected Mediterranean propolis samples. Is it a new type? LWT-Food Science and Technology, 65, 261-267.
• [10] de Lima, G.G., de Souza, R.O., Bozzi, A.D., Poplawska, M.A., Devine, D.M., Nugent, M.J. (2016). Extraction method plays critical role in antibacterial activity of propolis-loaded hydrogels. Journal of Pharmaceutical Sciences, 105(3), 1248-1257.
• [11] Taddeo, V.A., Epifano, F., Fiorito, S., Genovese, S. (2016). Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis. Journal of Pharmaceutical and Biomedical Analysis, 129, 219-223.
• [12] Trusheva, B., Trunkova, D., Bankova, V. (2007). Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry Central Journal, 1(1), 13.
• [13] Yang, W., Wu, Z., Huang, Z.Y., Miao, X. (2017). Preservation of orange juice using propolis. Journal of Food Science and Technology, 54(11), 3375-3383.
• [14] Bayram, N.E. (2018). Major constituents of different propolis samples. Hacettepe Journal of Biology and Chemistry, 45(4), 581-584.
• [15] Thamnopoulos, I.A.I., Michailidis, G.F., Fletouris, D.J., Badeka, A., Kontominas, M.G., Angelidis, A.S. (2018). Inhibitory activity of propolis against Listeria monocytogenes in milk stored under refrigeration. Food Microbiology, 73, 168-176.
• [16] da Silva, C., Prasniewski, A., Calegari, M.A., de Lima, V.A., Oldoni, T.L. (2018). Determination of total phenolic compounds and antioxidant activity of ethanolic extracts of propolis using ATR–FT-IR spectroscopy and chemometrics. Food Analytical Methods, 11(7), 2013-2021.
• [17] Cottica, S.M., Sabik, H., Antoine, C., Fortin, J., Graveline, N., Visentainer, J.V., Britten, M. (2015). Characterization of Canadian propolis fractions obtained from two-step sequential extraction. LWT-Food Science and Technology, 60(1), 609-614.
• [18] Ertürk, Ö., Yavuz, C., Sıralı, R. (2014). The antimicrobial activity of propolis from Ordu province of Turkey. Mellifera, 14(27-28), 11-16.
• [19] Segueni, N., Khadraoui, F., Rhouati, S. (2017). Volatile compounds as propolis characterization markers. In Euro-Mediterranean Conference for Environmental Integration, November, Springer, 1271-1273.
• [20] Ghamdi, A.A., Bayaqoob, N.I., Rushdi, A.I., Alattal, Y., Simoneit, B.R., El-Mubarak, A.H., Al-Mutlaq, K.F. (2017). Chemical compositions and characteristics of organic compounds in propolis from Yemen. Saudi Journal of Biological Sciences, 24(5), 1094-1103.
• [21] Bakdash, A., Almohammadi, O.H., Taha, N.A., Abu-Rumman, A., Kumar, S. (2018). chemical composition of propolis from the Baha Region in Saudi Arabia. Czech Journal of Food Science, 36(2), 00-10.
• [22] Mohtar, L.G., Rodríguez, S.A., Nazareno, M.A. (2017). Comparative analysis of volatile compound profiles of propolis from different provenances. Journal of the Science of Food and Agriculture, 98(9), 3409-3415.
• [23] Kapare, H., Lohidasan, S., Sinnathambi, A., Mahadik, K. (2019). Standardization, anti-carcinogenic potential and biosafety ofIndian propolis Journal of Ayurveda and Integrative Medicine, 10, 81-87.
• [24] Netíková, L., Bogusch, P., Heneberg, P. (2013). Czech ethanol‐free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. Journal of Food Science, 78(9), 1421-1429.
• [25] Arslan, S., Perçin, D., Silici, S., Er, Ö. (2010). Farklı çözücülerle hazırlanan propolis özütlerinin mutans streptokoklar üzerine in vitro antimikrobiyal etkisi. Sağlık Bilimleri Dergisi, 19(1), 68.
• [26] Ghisalberti E.L. (1979). Propolis: a review. Bee World, 60, 59–84.
• [27] Marcucci, M.C. (1995). Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83–99.
• [28] Hazem, A., Pıtıcă-Aldea, I.M., Popescu, C., Matei, L., Dragu, D., Economescu, M., Lupuliasa, D. (2017). The antiviral/virucidal effects of alcoholic and aqueous extracts with propolis. Farmacia, 65(6), 868-876.
• [29] Osés, S.M., Pascual-Maté, A., Fernández-Muiño, M.A., López-Díaz, T.M., Sancho, M.T. (2016). Bioactive properties of honey with propolis. Food Chemistry, 196, 1215-1223.
• [30] Suleman, T., Van Vuuren, S., Sandasi, M., Viljoen, A.M. (2015). Antimicrobial activity and chemometric modelling of South African propolis. Journal of Applied Microbiology, 119(4), 981-990.
• [31] Bankova, V., De Castro, S., Marcucci, M. (2000). Propolis: Recent advances in chemistry and plant origin. Apidologie, 31, 3–15.
• [32] Chen, Y.W., Wu, S.W., Ho, K.K., Lin, S.B., Huang, C.Y., Chen, C.N. (2008). Characterization of Taiwanese propolis collected from different locations and seasons. Journal of the Science of Food and Agriculture, 88, 412–419.
• [33] Kumazawa, S., Nakamura, J., Murase, M., Miyagawa, M., Ahn, M.R., Fukumoto, S. (2008). Plant origin of Okinawan propolis: honeybee behavior observation and phytochemical analysis. Naturwissenschaften, 95, 781–786.
• [34] Popova, M., Chen, C.N., Chen, P.Y., Huang, C.Y., Bankova, V. (2010). A validated spectrophotometric method for quantification of prenylated flavanones in Pacific propolis from Taiwan. Phytochemical Analysis, 21, 186–191.
• [35] Barros, M.P., Sousa, J.P., Bastos, J.K., Andrade, S.F. (2007). Effect of Brazilian green propolis on experimental gastric ulcers in rats. Journal of Ethnopharmacology, 110, 567–571.
• [36] Kumazawa S., Hamasaka T., Nakayama T. (2004). Antioxidant activity of propolis of various geographic origins, Food Chemistry, 84, 329–339.
• [37] Yang, H., Huang, Z., Huang, Y., Dong, W., Pan, Z., Wang, L. (2015). Characterization of Chinese crude propolis by pyrolysis–gas chromatography/mass spectrometry. Journal of Analytical and Applied Pyrolysis, 113, 158-164.
• [38] Yadav, H., Mungara, P., Jivrajani, M., Nivsarkar, M., Anandjiwala, S. (2012). TLC-densitometric quantification of negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol using HPTLC from vitex negundo leaves. Journal of Liquid Chromatography and Related Technologies, 35(11), 1565-1584.
• [39] Piccinelli, A.L., Mencherini, T., Celano, R., Mouhoubi, Z., Tamendjari, A., Aquino, R.P., Rastrelli, L. (2013). Chemical composition and antioxidant activity of Algerian propolis. Journal of Agricultural and Food Chemistry, 61(21), 5080-5088.
• [40] Moura, S.A.L., Negri, G., Salatino, A., Lima, L.D.C., Dourado, L.P.A., Mendes, J.B. (2009). Aqueous extract Brazilian propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges. Evidence-Based Complementary and Alternative Medicine, 18, 1–9.
• [41] Wang, R., Ding, S., Zhao, D., Wang, Z., Wu, J., Hu, W. (2016). Effect of dehydration methods on antioxidant activities, phenolic contents, cyclic nucleotides, and volatiles of jujube fruits. Food Science and Biotechnology, 25(1), 137-143.
• [42] Kang, L.J., Lee, H.B., Bae, H.J., Lee, S.G. (2010). Antidiabetic effect of propolis: reduction of expression of glucose‐6‐phosphatase through inhibition of Y279 and Y216 autophosphorylation of GSK‐3α/β in HepG2 cells. Phytotherapy Research, 24(10), 1554-1561.
• [43] de Castro, P.A., Savoldi, M., Bonatto, D., Malavazi, I., Goldman, M.H., Berretta, A.A. (2012). Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis. BMC Complementary and Alternative Medicine, 12, 194.
• [44] Nakajima, Y., Tsuruma, K., Shimazawa, M., Mishima, S., Hara, H. (2009). Comparison of bee products based on assays of antioxidant capacities. BMC Complementary and Alternative Medicine, 9, 4.
• [45] Ayala F, Lembo G, Nappa P, Balato N. (1985). Contact dermatitis from propolis. Contact Dermatitis, 12, 181–182.
• [46] Bankova V.S., Popov S.S., Marekov N.L. (1983). A study on flavonoids of propolis. Journal of Natural Products, 46, 471–474.
• [47] Bjorkner B.E. (1994). Industrial airborne dermatoses. Dermatology Clinics, 12, 501–509.
• [48] Dobrowolski J.W., Vohora S.B, Sharma K., Shah S.A., Naqvi S.A.H., Dandiya P.C. (1991). Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. Journal of Ethnopharmacology, 35, 77–82.
• [49] Burdock, G.A. (1998). Review of the biological properties and toxicity of bee propolis. Food Chemical Toxicology, 36, 347-363.
• [50] Çakıroğlu, T.N. (2010). Çeşitli çözücülerde Türk propolisinin çözünürlüğünün incelenmesi. Yüksek Lisans Tezi. Karadeniz Teknik Üniversitesi, Sağlık Bilimleri Enstitüsü, Trabzon.
• [51] Li, Y., Skouroumounis, G.K., Elsey, G.M., Taylor, D.K. (2011). Microwave- assistance provides very rapid and efficient extraction of grape seed polyphenols. Food Chemistry, 129(2), 570-576.
• [53] Büyüktuncel, E. (2012). Gelişmiş ekstraksiyon teknikleri. I. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 209-242.
• [54] Noureddine, H., Hage-Sleiman, R., Wehbi, B., Fayyad-Kazan, A.H., Hayar, S., Traboulssi, M., El Makhour, Y. (2017). Chemical characterization and cytotoxic activity evaluation of Lebanese propolis. Biomedicine & Pharmacotherapy, 95, 298-307.
• [56] Takeuchi, T.M., Pereira, C.G., Braga, M.E.M., Marostica, M.R., Leal, P.F., Meireles, M.A.A. (2008). Low pressure solvent extraction, microwave assisted, and ultrasound from condimentary plant. In: Extracting Bioactive Compounds for Food Products. CRC Press, 137-218.
• [57] Andrade, J.K.S., Denadai, M., de Oliveira, C.S., Nunes, M.L., Narain, N. (2017). Evaluation of bioactive compounds potential and antioxidant activity of brown, green and red propolis from Brazilian northeast region. Food Research International, 101, 129-138.
• [58] Rivera-Yañez, N., Rodriguez-Canales, M., Nieto-Yañez, O., Jimenez-Estrada, M., Ibarra-Barajas, M., Canales-Martinez, M.M., Rodriguez-Monroy, M.A. (2018). Hypoglycaemic and antioxidant effects of propolis of Chihuahua in a model of experimental diabetes. Evidence-Based Complementary and Alternative Medicine, Article ID 4360356, https://doi.org/10.1155/2018/4360356.
• [59] Turan, I., Demir, S., Misir, S., Kilinc, K., Mentese, A., Aliyazicioglu, Y., Değer, O. (2015). Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Tropical Journal of Pharmaceutical Research, 14(5), 777-782.
• [60] Yavuz, C. (2011). Türkiye’nin bazı illerinden toplanan propolislerin antimikrobiyal, antioksidan aktiviteleri ve biyoaktif bileşenlerinin tayini. Yüksek Lisans Tezi. Ordu Üniversitesi. Fen Bilimleri Enstitüsü, Ordu.
• [61] Maden Çalışkol, M. (2013). Azerbaycan yöresine ait propolis örneklerinin antioksidan özelliklerinin belirlenmesi. Yüksek Lisans Tezi. Karadeniz Teknik Üniversitesi. Fen Bilimleri Enstitüsü, Trabzon.
• [62] Kubiliene, L., Laugaliene, V., Pavilonis, A., Maruska, A., Majiene, D., Barcauskaite, K., Savickas, A. (2015). Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complementary and Alternative Medicine, 15(1), 156.
• [63] Bonvehí, J.S., Gutiérrez, A.L. (2011). Antioxidant activity and total phenolics of propolis from the Basque Country (Northeastern Spain). Journal of the American Oil Chemists' Society, 88(9), 1387-1395.
• [64] Skaba, D., Morawiec, T., Tanasiewicz, M., Mertas, A., Bobela, E., Szliszka, E., Makita, Y. (2013). Influence of the toothpaste with Brazilian ethanol extract propolis on the oral cavity health. Evidence-Based Complementary and Alternative Medicine, Article ID 215391, http://dx.doi.org/10.1155/2013/215391.
• [65] Yoshimasu, Y., Ikeda, T., Sakai, N., Yagi, A., Hirayama, S., Morinaga, Y., Nakao, R. (2018). Rapid bactericidal action of propolis against Porphyromonas gingivalis. Journal of Dental Research, 97(8), 928-936.
• [66] Temiz, A., Şener, A., Tüylü, A.Ö., Sorkun, K., Salih, B. (2011). Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology, 35(4), 503-511.
• [67] Duman, S. (2010). Çanakkale (Türkiye) ilinde toplanan propolis örneklerinin antimikrobiyal aktiviteleri üzerine çalışmalar. Yüksek Lisans Tezi. Çanakkale Onsekiz Mart Üniversitesi. Fen Bilimleri Enstitüsü, Çanakkale.
• [68] Karabulut E. (2011). Propolisin etanolik ekstresinin Helicobacter pylori ye karşı antimikrobiyal etkisinin araştırılması. Yüksek Lisans Tezi. Erciyes Üniversitesi, Fen Bilimleri Enstitüsü, Kayseri.
• [69] Apaydın, H. (2015). Propolisin hazır çorbalardan izole edilen Staphylococcus aureus üzerine inhibisyon etkisi Yüksek Lisans Tezi. Namık Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Tekirdağ.
• [70] Türk, M.U. (2017). Ege bölgesinde üretilen propolislerin kimyasal yapısı ve anti fungal aktivitesi. Yüksek Lisans Tezi. Uşak Üniversitesi. Fen Bilimleri Enstitüsü. Uşak.
• [71] Choi, Y.M., Noh, D.O., Cho, S.Y., Suh, H.J., Kim, K.M., Kim, J.M. (2006). Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT-Food Science and Technology, 39(7), 756-761.
• [72] Kartal, M., Yıldız, S., Kaya, S., Kurucu, S., Topçu, G. (2003). Antimicrobial activity of propolis samples from two different regions of Anatolia. Journal of Ethnopharmacology, 86(1), 69-73.
• [73] Monroy, Y. M., Rodrigues, R.A., Rodrigues, M.V., Sant’Ana, A.S., Silva, B.S., Cabral, F.A. (2017). Brazilian green propolis extracts obtained by conventional processes and by processes at high pressure with supercritical carbon dioxide, ethanol and water. The Journal of Supercritical Fluids, 130, 189-197.
• [74] Piotrowski, M., Pituch, H., Obuch-Woszczatyński, P. (2018). Antimicrobial effects of propolis on Clostridium difficile strains belonging to the different PCR-ribotypes. In Annales Universitatis Mariae Curie-Sklodowska, sectio C–Biologia, 71(2), 33.