Farklı Sınıf Türk Siyah Çaylarının Polifenol ve Alkaloid İçerikleri İle Antioksidan Aktivitesi (İngilizce)

Yıl 2010, Cilt: 35 Sayı: 3, 161 - 168, 01.06.2010

Nihal Turkmen Erol Ferda Sarı Y. Sedat Velioglu

Öz

Bu çalışmada yedi farklı sınıf Türk siyah çayının polifenol ve alkaloid içerikleri HPLC yöntemiyle, antioksidan aktiviteleri ise DPPH radikal yöntemiyle araştırılmıştır. Çay örneklerinde iki alkaloid (kafein ve teobromin), iki flavan-3-ol (EGCG ve ECG), üç flavonol glikoziti (Q3RG, Q3G and K3RG) ve dört teaflavin bileşiği (TF-f, TF-3-G, TF-3’-G and TF-3,3’-DG) tanımlanmış ve miktarları belirlenmiştir. Farklı sınıf çayların yapısında bulunan bileşiklerin miktarlarının ve bunların antioksidan aktivitelerinin istatistikî olarak anlamlı düzeyde farklı olduğu ortaya konulmuştur. Genel olarak çaylarda bulunan alkaloidlerin, Q3RG, Q3G ve teaflavinler ile antioksidatif aktivitenin 1., 2. 3. ve 7. sınıf çaylarda, buna karşın flavan-3-ol’lerin ise 4., 5. ve 6. sınıf çaylarda daha yüksek olduğu saptanmıştır. K3RG açısından çay sınıfları arasında farklılık görülmemiştir.

Anahtar Kelimeler

Siyah çay, alkaloid, fenolik bileşik, çay sınıfı, antioksidan aktivite

Polyphenols, Alkaloids and Antioxidant Activity of Different Grades Turkish Black Tea (in English)

Öz

The content of polyphenols and alkaloids of seven grades Turkish black tea was investigated as well as their antioxidant activity. Polyphenol and alkaloid contents of teas were determined using HPLC method. Antioxidant activity was evaluated by DPPH radical assay. In tea samples examined, two alkaloids (caffeine and theobromine), two flavan-3-ols (EGCG and ECG), three flavonol glycosides (Q3RG, Q3G and K3RG) and four individual theaflavins (TF-f, TF-3-G, TF-3’-G and TF-3,3’-DG) were identified and quantified. Significant differences were detected in biochemicals content and antioxidant activity between different grades black teas. In general, the contents of alkaloids, Q3RG, Q3G and theaflavins in 1st, 2nd, 3rd and 7th grade teas and the contents of flavan-3-ols in 4th, 5th and 6th grade teas were found to be higher. However, there was no significant difference in K3RG content of the teas. 1st, 2nd, 3rd and 7th grade teas showed also higher antioxidant activity.

Anahtar Kelimeler

Black tea, alkaloid, phenolics, tea grade, antioxidant activity

Kaynakça

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