Araştırma Makalesi

Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye

Sayı: 2026 18 Eylül 2026
PDF İndir
TR EN

Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye

Öz

Myxozoans represent an important group of metazoan endoparasites. Tetracapsuloides bryosalmonae is a myxozoan parasite belonging to the class Malacosporea and is the causative agent of proliferative kidney disease in salmonid fish. This parasite poses a serious threat to both farmed and wild salmonid populations in Europe and North America. In Türkiye, there has been no report on the presence of T. bryosalmonae in salmonid fish to date. This study aimed to investigate, for the first time, the presence of T. bryosalmonae in farmed rainbow trout (Oncorhynchus mykiss) reared in floating net cages in Derbent Dam Lake, Samsun, Türkiye using molecular methods. Between July 2024 and February 2025, necropsies were performed on a total of 150 rainbow trout weighing 150–250 g. Tissue samples were collected from the anterior and posterior kidneys of each fish, and DNA isolation was performed from these tissues. The presence of T. bryosalmonae DNA in kidney-derived DNA samples was screened using multiplex PCR. Tetracapsuloides bryosalmonae DNA was not detected in any of the 150 rainbow trout samples examined by molecular screening. This study provides the first epidemiological data indicating that T. bryosalmonae was not detected in rainbow trout farmed in Derbent Dam Lake, Samsun. However, more comprehensive molecular screening studies are needed to determine the presence of T. bryosalmonae in both farmed salmonid species and wild salmonid populations inhabiting natural environments across different geographical regions of Türkiye.

Anahtar Kelimeler

Rainbow trout, Tetracapsuloides bryosalmonae, PCR, Türkiye

Destekleyen Kurum

Ondokuz Mayıs University

Proje Numarası

BAP04-A-2024-5592

Etik Beyan

I declare that an ethics committee approval certificate is not required for our research paper entitled "Molecular Screening of Tetracapsuloides bryosalmonae (Malacosporea) in Rainbow Trout (Oncorhynchus mykiss) Farmed from Samsun, Türkiye" because we used material from dead rainbow trout.

Teşekkür

This study was supported by Ondokuz Mayıs University under project number BAP04-A-2024-5592.

Kaynakça

  1. Bettge, K., Segner, H., Burki, R., Schmidt-Posthaus, H., & Wahli, T. (2009). Proliferative kidney disease (PKD) of rainbow trout: Temperature- and time- related changes of Tetracapsuloides bryosalmonae DNA in the kidney. Parasitology, 136(5), 615-625. https://doi.org/10.1017/S0031182009005800
  2. Canning, E.U., Curry, A., Feist, S.W., Longshaw, M., & Okamura, B. (1999). Tetracapsula bryosalmonae n. sp. for PKX organism, the cause of PKD in salmonid fish. Bulletin of the European Association of Fish Pathologists, 19, 203-206.
  3. Dash, M., & Vasemägi, A. (2014). Proliferative kidney disease (PKD) agent Tetracapsuloides bryosalmonae in brown trout populations in Estonia. Diseases of Aquatic Organisms, 109(2), 139-148. https://doi.org/10.3354/dao02731
  4. Eszterbauer, E., Atkinson, S., Diamant, A., Morris, D., El- Matbouli, M., & Hartikainen, H. (2015). Myxozoan life cycles: practical approaches and insights, In: Okamura, B., Gruhl, A. & Bartholomew J.L. (Ed), Myxozoan evolution, ecology and development, 175-198p, Springer International Publishing. https://doi.org/10.1007/978-3-319-14753-6_10
  5. Feist, S.W., & Longshaw, M. (2006). Phylum myxozoa, In: Woo P.T.K. (Ed), Fish diseases and disorders, Vol 1, 2nd ed, 230-296p, UK: CAB International. https://doi.org/10.1079/9780851990156.0230
  6. Fiala, I., Bartošová-Sojková, P., & Whipps, C.M. (2015). Classification and phylogenetics of myxozoa, In: Okamura, B., Gruhl, A. & Bartholomew J.L. (Ed), Myxozoan evolution, ecology and development, 85-110p, Springer International Publishing. https://doi.org/10.1007/978-3-319-14753-6_5
  7. Gorgoglione, B., Kotob, M.H., Unfer, G., & El-Matbouli, M. (2016). First proliferative kidney disease outbreak in Austria, linking to the aetiology of Black Trout Syndrome threatening autochthonous trout populations. Diseases of Aquatic Organisms, 119(2). 117-128. https://doi.org/10.3354/dao02993
  8. Grabner, D.S., & El-Matbouli, M. (2008). Transmission of Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea) to Fredericella sultana (Bryozoa: Phylactolaemata) by various fish species. Diseases of Aquatic Organisms, 79(2). 133-139. https://doi.org/10.3354/dao01894
  9. Grabner, D.S., & El-Matbouli, M. (2009). Comparison of the susceptibility of brown trout (Salmo trutta) and four rainbow trout (Oncorhynchus mykiss) strains to the myxozoan Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (PKD). Veterinary Parasitology, 165(3-4). 200-206. https://doi.org/10.1016/j.vetpar.2009.07.028
  10. Hartikainen, H., & Okamura, B. (2015). Ecology and evolution of malacosporean-bryozoan interactions, In: Okamura, B., Gruhl, A. & Bartholomew J.L. (Ed), Myxozoan evolution, ecology and development, 201-216 p, Springer International Publishing. https://doi.org/10.1007/978-3-319-14753-6_11

Kaynak Göster

APA
Pekmezci, G. Z., & Yüksel, O. (2026). Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.2001531
AMA
1.Pekmezci GZ, Yüksel O. Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye. Journal of Anatolian Environmental and Animal Sciences. 2026;(2026). doi:10.35229/jaes.2001531
Chicago
Pekmezci, Gökmen Zafer, ve Onur Yüksel. 2026. “Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye”. Journal of Anatolian Environmental and Animal Sciences, sy 2026. https://doi.org/10.35229/jaes.2001531.
EndNote
Pekmezci GZ, Yüksel O (01 Eylül 2026) Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye. Journal of Anatolian Environmental and Animal Sciences 2026
IEEE
[1]G. Z. Pekmezci ve O. Yüksel, “Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye”, Journal of Anatolian Environmental and Animal Sciences, sy 2026, Eyl. 2026, doi: 10.35229/jaes.2001531.
ISNAD
Pekmezci, Gökmen Zafer - Yüksel, Onur. “Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye”. Journal of Anatolian Environmental and Animal Sciences. 2026 (01 Eylül 2026). https://doi.org/10.35229/jaes.2001531.
JAMA
1.Pekmezci GZ, Yüksel O. Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye. Journal of Anatolian Environmental and Animal Sciences. 2026. doi:10.35229/jaes.2001531.
MLA
Pekmezci, Gökmen Zafer, ve Onur Yüksel. “Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye”. Journal of Anatolian Environmental and Animal Sciences, sy 2026, Eylül 2026, doi:10.35229/jaes.2001531.
Vancouver
1.Gökmen Zafer Pekmezci, Onur Yüksel. Molecular Screening for Tetracapsuloides bryosalmonae (Malacosporea) in Farmed Rainbow Trout (Oncorhynchus mykiss) from Samsun, Türkiye. Journal of Anatolian Environmental and Animal Sciences. 01 Eylül 2026;(2026). doi:10.35229/jaes.2001531