Peroksidaz benzeri aktivite ve antibakteriyel uygulamalar için Stellaria media temelli biyoaktif hibrit nanoçiçeklerin sentezi

Öz

Bu çalışmada, basit bir biyomimetik yaklaşım kullanılarak Stellaria media (L.)Vill.(1789) (Sm) aracılığıyla Cu₃(PO₄)₂ hibrit nanoflower'lar (hNF'ler) başarıyla sentezlendi. Sentezlenen SmhNF'ler kapsamlı bir şekilde karakterize edildi. Görüntüleme analizleri, çok katmanlı taç yaprağı mimarisine sahip hiyerarşik çiçek benzeri nanoyapıların sentezini doğruladı. Dinamik ışık saçılımı (DLS) ve zeta potansiyeli analizleri, nispeten homojen bir parçacık dağılımı ve negatif yüklü yüzey özelliklerini ortaya koydu. FTIR spektrumları, SmhNF oluşumu sırasında fitokimyasal bileşikler ve Cu-fosfat yapıları arasındaki etkileşimi doğrulayarak, hidroksil, karbonil, fosfat ve Cu–O ile ilgili fonksiyonel grupların varlığını gösterdi. Bu çalışma ile sentezlenen SmhNF'lerin önemli peroksidaz benzeri katalitik aktivite sergilediği tespit edildi. Ek olarak, Klebsiella pneumoniae ve Streptococcus pneumoniae'ye karşı antibakteriyel aktivite değerlendirildi. Sonuçlar, SmhNF'lerin saf Cu₃(PO₄)₂ kristalleri ve tek başına bitki özütüne kıyasla gelişmiş antibakteriyel performans sergilediğini ortaya koymuştur. Geliştirilmiş biyolojik aktivite, Cu²⁺ iyonlarının sinerjik etkileri, reaktif oksijen türlerinin oluşumu ve hibrit nanoflower mimarisinin yüksek yüzey alanı ile açıklanmaktadır.

Anahtar Kelimeler

• Stellaria Media
• hibrit nanoflower
• katalitik aktivite
• antibakteriyel aktivite.
• Cu₃(PO₄)₂ hibrit yapıları

Synthesis of Stellaria media-based bioactive hybrid nanoflowers for peroxidase-like activity and antibacterial applications

Öz

In this study, Cu₃(PO₄)₂ hybrid nanoflowers(hNFs) were successfully synthesized via Stellaria media (L.) Vill. (1789) (Sm) using a simple biomimetic approach. The synthesized SmhNFs were comprehensively characterized. Imaging analyses confirmed the synthesis of hierarchical flower-like nanostructures with a multilayered petal architecture. Dynamic light scattering (DLS) and zeta potential analyses revealed a relatively homogeneous particle distribution and negatively charged surface properties. FTIR spectra confirmed the interaction between phytochemical compounds and Cu-phosphate structures during SmhNF formation, showing the presence of hydroxyl, carbonyl, phosphate, and Cu–O related functional groups. This study demonstrated that the synthesized SmhNFs exhibited significant peroxidase-mimic catalytic activity. Additionally, antibacterial activity was evaluated against Klebsiella pneumoniae and Streptococcus pneumoniae. The results revealed that SmhNFs exhibited enhanced antibacterial performance compared to pure Cu₃(PO₄)₂ crystals and plant extract alone. The improved biological activity is explained by the synergistic effects of Cu²⁺ ions, formation of reactive oxygen species, and high surface area of hybrid nanoflower architecture.

Anahtar Kelimeler

• Stellaria media
• hybrid nanoflower
• catalytic activity
• antibacterial activity
• Cu₃(PO₄)₂ hybrid structure.

Kaynakça

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