FARKLI KAYNAKLARDAN İZOLE EDİLMİŞ LAKTİK ASİT BAKTERİLERİNİN EKZOPOLİSAKKARİT ÜRETİMİ VE KOLESTEROL ASİMİLASYON YETENEKLERİNİN BELİRLENMESİ

Year 2019, Volume: 44 Issue: 2, 191 - 201, 15.04.2019

Duygu Alp , Hakan Kuleaşan

https://doi.org/10.15237/gida.GD18059

Cited By: 3

Abstract

Bağırsak sistemimizde yer alan yararlı
mikroorganizmaların önemli özelliklerinden birisi gastrointestinal sistemdeki
farklı koşullara karşı direnç gösterebilmeleridir. Bu koşullar arasında safra
tuzlarına karşı direnç ve düşük pH’da gelişim yer almaktadır. Gastrointestinal
sistemde canlılıklarını sürdürebilmek için yararlı bakteriler spesifik enzim
sentezi veya ekzopolisakkarit üretimi gibi çeşitli savunma mekanizmaları
geliştirmişlerdir. Bu çalışmada çeşitli fermente gıdalardan ve bitkilerden
izole edilmiş laktik asit bakterilerinin safra tuzu dirençleri, düşük pH’da
gelişim özelliklerinin yanı sıra, ekzopolisakkarit üretimleri ve kolesterol
asimilasyon yetenekleri belirlenmiştir. Ekzopolisakkarit (EPS) üretimlerinin
11.9 ile 1.1 mg/L arasında değiştiği belirlenirken toplam kolesterol
asimilasyon yüzdeleri % 60.71 ile % 16.71 arasında değişim göstermiştir.
Kolesterol asimilasyon yetenekleri ile EPS üretimleri arasında bir bağlantı
bulunamamıştır. 

Keywords

Kolesterol Asimilasyonu, Ekzopolisakkarit, düşük pH, safra tuzu direnci

DETERMINATION OF EXCOPOLISACCHARIDE PRODUCTION AND CHOLESTEROL ASSIMILATION ABILITIES OF LACTIC ACID BACTERIA ISOLATED FROM DIFFERENT SOURCES

Abstract

One of the most important features of the intestinal
flora is resistance to many conditions in the gastrointestinal tract. These
include resistance to bile salt and growth at low pH. Bacteria have developed
various defense mechanisms, such as specific enzymes, exopolysaccharide
production, against Gastrointestinal system, In our study, strains of
lactic acid bacteria  isolated
from various fermented foods and plants and all the strains were determined of
their bile salt and low pH resistance. The strains were also tested for their
exopolysaccharide production and total cholesterol assimilation properties. EPS
production was observed at rations between 11.9 to 1.1 mg/L, and total
cholesterol assimilation percentages ranged from 60.71 to 16.71. There was no
relevance between cholesterol assimilation ability and their EPS production.

Keywords

Cholesterol assimilation, low pH, exopolysaccharide, bile salt resistance

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