DETERMINATION OF THE ANTIBIOTIC RESISTANCE PROFILES OF HIGH-LEVEL AMINOGLYCOSID-RESISTANT ENTEROCOCCI ISOLATED FROM BROILER MEAT

Yıl 2021, Cilt: 46 Sayı: 4, 803 - 816, 17.05.2021

Meltem Yalçın , Yasin Tuncer

https://doi.org/10.15237/gida.GD21063

Öz

In this study, the prevalence of high-level aminoglycoside-resistant (HLAR) Enterococcus in broiler meat samples obtained from Antalya and Isparta provinces was investigated and antibiotic resistance profiles of isolates was detected. A total of 32 HLAR Enterococcus strains were isolated in this study. Isolates were identified as 18 E. faecium, 5 E. faecalis, 5 E. durans, 3 E. avium and 1 E. casseliflavus by molecular methods. As a result of the disc diffusion test, HLAR Enterococcus isolates were found to be most susceptible to ampicillin (93.75%), linezolide (93.75%), penicillin G (90.62%), teikoplanine (90.62%), nitrofurantoin (78.12%), vancomycin (75%) and chloramphenicol (68.75%). It has been determined that the antibiotics to which the isolates are most resistant are erythromycin (96.87%), minocycline (96.87%), streptomycin (96.87%), and tetracycline (96.87%). Gentamicin and streptomycin minimum inhibitory concentration (MIC) values of HLAR isolates were detected changing in the ranged from 16 to >4096 and 64 to >4096 µg/mL, respectively. According to the MIC test results, 18 out of 32 HLAR isolates were identified as both high-level gentamicin-resistant (HLGR) and high-level streptomycin-resistant (HLSR).

Anahtar Kelimeler

Chicken meat , Enterococcus , High level aminoglycoside resistance , Antibiotic resistance , Polymerase chain reaction (PCR)

Proje Numarası

5010-YL1-17

PİLİÇ ETİNDEN İZOLE EDİLEN YÜKSEK SEVİYEDE AMİNOGLİKOZİT DİRENÇLİ ENTEROKOKLARIN ANTİBİYOTİK DİRENÇ PROFİLLERİNİN BELİRLENMESİ

Öz

Bu çalışmada, Antalya ve Isparta illerinden temin edilen 112 piliç eti örneğinde yüksek seviyede aminoglikozit dirençli (YSAD) Enterococcus yaygınlığı araştırılmış ve izolatların antibiyotik direnç profilleri belirlenmiştir. Çalışmada toplam 32 YSAD Enterococcus suşu izole edilmiştir. Moleküler yöntemler ile izolatların 18’i E. faecium, 5’i E. faecalis, 5’i E. durans, 3’ü E. avium ve 1’i E. casseliflavus olarak tanımlanmıştır. Disk difüzyon testi sonucu, izolatların en duyarlı olduğu antibiyotiklerin ampisilin (%93.75), linezolid (%93.75), penisilin G (%90.62), teikoplanin (%90.62), nitrofurantoin (%78.12), vankomisin (%75) ve kloramfenikol (%68.75) olduğu belirlenmiştir. İzolatların en dirençli olduğu antibiyotiklerin ise eritromisin (%96.87), minosiklin (%96.87), streptomisin (%96.87) ve tetrasiklin (%96.87) olduğu tespit edilmiştir. İzolatların gentamisin ve streptomisin minimum inhibisyon konsantrasyonu (MİK) değerlerinin sırasıyla 16 ile >4096 ve 64 ile >4096 µg/mL arasında değiştiği belirlenmiştir. MİK testleri sonucu, 32 YSAD Enterococcus izolatının 18’inin hem yüksek seviyede streptomisin dirençli (YSSD) hem de yüksek seviyede gentamisin dirençli (YSGD) oldukları tespit edilmiştir.

Anahtar Kelimeler

Tavuk eti , Enterococcus , Yüksek seviyede aminoglikozid direnci , antibiyotik direnç , polimeraz zincir reaksiyonu (PZR)

Destekleyen Kurum

Süleyman Demirel Üniversitesi

Proje Numarası

5010-YL1-17

Teşekkür

Bu çalışmayı 5010-YL1-17 nolu proje ile maddi olarak destekleyen Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi’ne teşekkür ederiz.

Kaynakça

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