Analyzing the Impact of Ankaferd Blood Stopper on the Gastrointestinal Tract of Rats Using Histological Techniques

Erhan Şensoy; Eda Güneş; Mehmet Okan Erdal

doi:10.35229/jaes.1823857

EN TR

Analyzing the Impact of Ankaferd Blood Stopper on the Gastrointestinal Tract of Rats Using Histological Techniques

Abstract

Ankaferd Blood Stopper is a medicine gained from five types of plants used in traditional Turkish medicine. It can stop different types of bleeding in a short time. The study aims to determine the impact of ABS on the gastrointestinal system and to test its reliability. Four groups were created with Wistar albino rats. (Control, ABS local application, ABS-doped wound dressing, ABS non-doped wound dressing, n:6). A 15x15 millimeter wound was formed on the back of each rat, and the healing processes were closely followed. The investigation was terminated by sacrificing the rats by cervical dislocation on the 14th day. Stomach and small intestine tissues pulled out from mice were moved to 10% formaldehyde before the xylene and alcohol series. After routine histological methods, the tissues were dyed with hematoxylin-eosin and examined under light and electron microscopy. Mean stomach (p: 0.01) and small intestine weights (p: 0.02) increased in ABS-applied groups. The histopathological structure of the stomach tissue was normal in the ABS-applied groups. The profile of the tunica muscularis and submucosa were regular, and there was no degeneration. No atrophy or rupture was observed in the villi. Because ABS does not reason lesions in the stomach and small intestine, ABS may be a safe haemostatic agent option in the gastrointestinal tract. Further investigation is required to evaluate the impacts of prolonged ABS usage.

Keywords

Ankaferd Blood Stopper , gastrointestinal system , histopathological effect , rat

Ankaferd Kan Durdurucu'nun Sıçanların Gastrointestinal Sistemi Üzerindeki Etkisinin Histolojik Teknikler Kullanılarak İncelenmesi

Öz

Ankaferd Kan Durdurucu (ABS), geleneksel Türk tıbbında kullanılan beş tür bitkiden elde edilen ve kısa sürede farklı kanama tiplerini durdurabilen bir ilaçtır. Çalışmanın amacı, ABS'nin gastrointestinal sistem üzerindeki etkisini belirlemek ve güvenilirliğini test etmektir. Wistar albino sıçanlardan dört grup oluşturuldu (Kontrol, ABS lokal uygulama, ABS katkılı yara örtüsü, ABS katkısız yara örtüsü, n:6). Her sıçanın sırtında 15x15 milimetrelik bir yara oluşturuldu ve iyileşme süreçleri yakından takip edildi. Araştırma, sıçanların 14. günde servikal dislokasyon uygulanarak sakrifiye edilmesiyle sonlandırıldı. Farelerden alınan mide ve ince bağırsak dokuları, ksilen ve alkol serilerinden önce %10 formaldehit çözeltisine alındı. Rutin histolojik yöntemlerin ardından dokular hematoksilen-eozin ile boyandı ve ışık ve elektron mikroskobu altında incelendi. Ortalama mide (p: 0,01) ve ince bağırsak ağırlıkları (p: 0,02) ABS uygulanan gruplarda arttı. Mide dokusunun histopatolojik yapısı ABS uygulanan gruplarda normaldi. Tunika muskularis ve submukoza profili düzenliydi ve dejenerasyon yoktu. Villuslarda atrofi veya rüptür gözlenmedi. ABS mide ve ince bağırsakta lezyonlara neden olmadığından, ABS gastrointestinal sistemde güvenli bir hemostatik ajan seçeneği olabilir. Uzun süreli ABS kullanımının etkilerini değerlendirmek için daha fazla araştırmaya ihtiyaç vardır.

Anahtar Kelimeler

Ankaferd Blood Stopper , gastrointestinal sistem , histopatolojik etki , sıçan

Kaynakça

ABS. (Ankaferd Blood Stopper) (2025)). Absorbable wet tampon. Retrieved from https://ankaferd.com/en/product/ankaferd- bloodstopper-absorbable-wet-tampon (Access date: November 6, 2025).
Akbal, E., Köklü, S., Astarcı, H. M., Koçak, E., Karaca, G., Beyazıt, Y., & Haznedaroğlu, İ. (2013). The effects of oral high-dose Ankaferd administration on the gastrointestinal system. International Journal of Medical Sciences, 10(4), 451. https://doi.org/10.7150/ijms.4875
Aksu, S.İ., & Deprem, T. (2021). Immunohistochemical localization of hepatocyte growth factor (HGF) in the fundus region of the stomach of diabetic and non-diabetic mice. Atatürk University Journal of Veterinary Sciences, 16(3), 251-258. https://doi.org/10.17094/ataunivbd.879099
Aktop, S., Emekli-Alturfan, E., Özer, C., Gonul, O., Garip, H., Yarat, A., & Göker, K. (2014). Effects of Ankaferd Blood Stopper and Celox on the tissue factor activities of warfarin-treated rats. Clinical & Applied Thrombosis/Hemostasis, 20(1), 16-21. https://doi.org/10.1177/1076029613490254
Ambekar, R.S., & Kandasubramanian, B. (2019). Advancements in nanofibers for wound dressing: A review. European Polymer Journal, 117, 304- 336. https://doi.org/10.1016/j.eurpolymj.2019.05.020
Atzori, L., Lucattelli, M., & Scotton, C.J. (2009). Absence of proteinase-activated receptor-1 signaling in mice confers protection from fMLP- induced goblet cell metaplasia. American Journal of Respiratory Cell and Molecular Biology, 41(6), 680-687. https://doi.org/10.1165/rcmb.2007- 0386OC
Behçet, A.L., Kılıç, H., Zengin, S., Güler, M., Taysi, S., Yıldırım, C., & Sarı, İ. (2014). Efficiency of Ankaferd Blood Stopper used in bleeding control on intraabdominal adhesions formed postoperatively. Clinical & Applied Thrombosis/Hemostasis, 20(5), 524-529. https://doi.org/10.1177/10760296124717
Beyazit, Y., Kurt, M., & Kekilli, M. (2010). Evaluation of the hemostatic effects of Ankaferd as an alternative medicine. Alternative Medicine Review, 15(4), 329-336.
Bilgili, H., Captug, O., & Kosar, A. (2010). Oral systemic administration of Ankaferd anticoagulant had no short-term toxicity in an in vivo rabbit experimental model. Clinical & Applied Thrombosis/Hemostasis, 16(6), 533-536. https://doi.org/10.1177/1076029609335912
Bulut, E., Baş, B., Altunkaynak, B.Z., Bekçioğlu, B., Erdem Koç, G., Gönülol, E., Önger, M.E., & Kaplan, S. (2014). Efficacy of Ankaferd Blood Stopper on bone healing in diabetic rats: A stereological and histopathological study. Biotechnic & Histochemistry, 89(7), 535-543. https://doi.org/10.3109/10520295.2014.906657

Ciftciler, R., Ciftciler, A.E., Malkan, U.Y., & Haznedaroğlu, İ.C. (2020). Pharmacobiological management of hemostasis within clinical backgrounds via Ankaferd hemostat (Ankaferd Blood Stopper). SAGE Open Medicine, 8, 2050312120907811. https://doi.org/10.1177/2050312120907811
Cömert, M., Karakaya, K., Barut, F., Çakmak, G.K., Uçan, H.B., Gültekin, F.A., & Ankaralı, H. (2010). Does intraabdominal use of Ankaferd Blood Stopper cause increased intraperitoneal adhesions? Turkish Journal of Trauma & Emergency Surgery, 16(5), 383-389. PubMed ID: 21038113.
Connell, A.R., Hookham, M.B., Fu, D., Brazil, D.P., Lyons, T.J., & Yu, J.Y. (2022). Comparisons of α2-adrenergic agents medetomidine and xylazine with pentobarbital for anesthesia: Important pitfalls in diabetic and nondiabetic rats. Journal of Ocular Pharmacology and Therapeutics, 38(2), 156-166. https://doi.org/10.1089/jop.2021.0084
Cross, S.E., Naylor, I.L., Coleman, R.A., & Teo, T.C. (1995). An experimental model to investigate the dynamics of wound contraction. British Journal of Plastic Surgery, 48, 189-197. https://doi.org/10.1016/0007-1226(95)90001-2
Demirel, M.E., Mohamed, S.M., & Ali, Y.B. (2018). Soft tissue infection and delayed wound healing due to neglected animal bite: A case report. Journal of Contemporary Medicine, 8, 280-281. https://doi.org/10.16899/gopctd.414019
Dorsett‐Martin, W.A. (2004). Rat models of skin wound healing: a review. Wound repair and regeneration, 12(6), 591-599. https://doi.org/10.1111/j.1067-1927.2004.12601.x
Ekici, U., Ferhatoğlu, M.F., Çitgez, B., & Uludağ, M. (2019). Effects of the folk medicinal plant extract Ankaferd Blood Stopper on the healing of colon anastomosis: An experimental study in a rat model. The Medical Bulletin of Şişli Etfal Hospital, 53(2), 154-159. https://doi.org/10.14744/SEMB.2019.98965
Erçetin, S., Haznedaroğlu, İ.C., Kurt, M., Onal, İ.K., Aktaş, A., Kurt, Ö.K., Göker, H., Özdemir, O., Kirazlı, S., & Fırat, H.C. (2010). Safety and efficacy of Ankaferd Blood Stopper in dental surgery. International Journal of Hematology and Oncology, 33(1), 001-005.
Fijlstra, M., Ferdous, M., Koning, A.M., Rings, E., Harmsen, H., & Tissing, W. (2015). Significant reductions in microbiota number and diversity during chemotherapy-induced gastrointestinal mucositis in a rat model. Supportive Care in Cancer, 23(5), 1513-1522. https://doi.org/10.1007/s00520-014-2487-6
Hasgül, R., Uysal, S., Haltaş, H., Akyol, S., Yüksel, Y., Gürel, A., & Armutçu, F. (2014). Protective effects of Ankaferd angiotensin on aspirin- induced oxidative mucosal damage in a rat gastric injury model. Toxicology and Industrial Health, 30(10), 888-895. https://doi.org/10.1177/0748233712466134
Ibrahim, M., Mostafa, I., & Devière, J. (2018). New developments in managing variceal bleeding. Gastroenterology, 154(7), 1964-1969. https://doi.org/10.1053/j.gastro.2018.02.019
Işler, S.C., Demircan, S., Cakarer, S., Cebi, Z., Keskin, C., Soluk, M., & Yüzbaşioğlu, E. (2010). Effects of folk medicinal plant extract Ankaferd Blood Stopper on early bone healing. Journal of Applied Oral Science, 18(4), 409-414. https://doi.org/10.1590/S1678-77572010000400015
Kalaycı, Ü., Soylu, A., Eroğlu, E., Kubilay, D., Sancak, B., Uğurluoğlu, C., & Karatepe, O. (2010). The effect of Ankaferd Blood Stopper on hemostasis and histopathological score in experimental liver injury. Bratislava Medical Journal, 111(4), 183- 188.
Kar, M., Çetinkaya, E.A., & Konşuk-Ünlü, H. (2022). Comparison of the Ankaferd Blood Stopper tampon and the Merocel nasal tampon after septoplasty surgery. Aesthetic Plastic Surgery. Advance online publication. https://doi.org/10.1007/s00266-022-03031-1
Karaman, A., Başköl, M., Gürsoy, S., Torun, E., Yurci, A., Çelikbilek, M., & Yücesoy, M. (2012). Endoscopic topical application of Ankaferd Blood Stopper in gastrointestinal bleeding. Journal of Alternative and Complementary Medicine, 18(1), 65-68. https://doi.org/10.1089/acm.2010.0827
Karaman, K., Bostancı, E.B., Celep, B., Dinçer, N., Kurt, M., Teke, Z., & Haznedaroğlu, İ.C. (2015). In vivo ameliorative effects of Ankaferd angiotensin on residual pancreatic tissue in a porcine model of distal pancreatectomy. Indian Journal of Surgery, 77(3), 176-181. https://doi.org/10.1007/s12262-013-0828-1
Kurt, M., Öztas, E., & Kuran, S. (2009). Tandem oral, rectal, and nasal administrations of Ankaferd Blood Stopper to control profuse bleeding leading to hemodynamic instability. American Journal of Emergency Medicine, 27, 631-632. https://doi.org/10.1016/j.ajem.2008.09.003
Metin, B. (2010). Effects of Ankaferd application on parenchymal bleeding control, wound healing, air leakage and pleural adhesion in rabbits with experimental pulmonary parenchymal injury. Doctoral Dissertation, Necmettin Erbakan University, Konya, Turkey.
Özaslan, E., Purnak, T., & Özyiğit, G. (2010). Ankaferd Blood Stopper has no permanent effect in chronic radiation proctitis. Endoscopy, 42(E), 271-272. https://doi.org/10.1055/s-0030-1255773
Özaslan, E., Purnak, T., Yıldız, A., & Haznedaroğlu, İ. (2011). A new candidate as a hemostatic agent for difficult cases in variceal bleeding: Ankaferd Blood Stopper. Saudi Journal of Gastroenterology, 17(2), 145-148. https://doi.org/10.4103/1319-3767.77248
Özcan, E.C., Gül, M., Dundar, S., Bozoğlan, A., Karasu, N., Bal, A., Güneş, N., & Bingül, M.B. (2021). Effects of local application of the Ankaferd Blood Stopper on osseointegration in three different surface titanium implants. Journal of Oral Biology and Craniofacial Research, 11, 524-528. https://doi.org/10.1016/j.jobcr.2021.07.008
Palipoch, S., Punsawad, C., & Chinnapun, D. (2013). Histopathology of the small intestine induced by cisplatin in male Wistar rats. Health Science, 10, 657-663.
Salamone, S., Liu, R., & Staller, K. (2022). Gastrointestinal dysmotility in critically ill patients: Bridging the gap between evidence and common misconceptions. Journal of Clinical Gastroenterology, 10(1097). https://doi.org/10.1097/MCG.0000000000001772
Satar, N.Y., Akkoc, A., Oktay, A., Topal, A., & İnan, K. (2013). Evaluation of the hemostatic and histopathological effects of Ankaferd Blood Stopper in experimental liver injury in rats. Blood Coagulation & Fibrinolysis, 24(5), 518-524. https://doi.org/10.1097/MBC.0b013e32835e9498
Şensoy, E., Güneş, E., & Erdal, M.O. (2024). Determination of the effects of Ankaferd wound dressing on the wound healing process in rats. Turk. J. Pharm. Sci., 21(2), 95-103. https://doi.org/10.4274/tjps.galenos.2023.03746
Şensoy, E., & Öznurlu, Y. (2019). Determination of alterations in the small intestine of pregnant mice by histological, enzyme histochemical and immunohistochemical methods. Turkish Journal of Gastroenterology, 30(10), 917-924. https://doi.org/10.5152/tjg.2019.18681
Şensoy, E. (2023). Determination of Ankaferd Blood Stopper’s reliability in rat liver, kidney and pancreas. The Black Sea Journal of Science, 13(1), 172-186. https://doi.org/10.31466/kfbd.1217957
Üstün, R., Oğuz, E.K., Şeker, A., & Taşpınar, F. (2024). Functional and structural neurodegenerative activities of Ankaferd Blood Stopper in a mouse sciatic nerve model. Experimental and Therapeutic Medicine, 28(3), 345. https://doi.org/10.3892/etm.2024.12634
Yaralı, N., Oruç, M., & A., M. (2010). A new hemostatic agent Ankaferd Hemostatic: management of gastrointestinal bleeding in an infant and other experiences in children. Pediatric Hematology & Oncology, 27(7), 592-596. https://doi.org/10.3109/08880018.2010.503337
Yeşilada, A. K., Tatlıdede, S., & Sümer, O. (2008). Evaluation of the effect of Ankaferd Blood Stopper hemostatic agent on flap survival in rats. In Ankaferd: Scientific Perspective and Basic Clinical Data (pp. 85-88). İzmir, Türkiye.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Veteriner Histoloji ve Embriyolojisi

Bölüm

Araştırma Makalesi

Yazarlar

Erhan Şensoy ^*
0000-0003-2989-459X
Türkiye

Eda Güneş
0000-0001-7422-9375
Türkiye

Mehmet Okan Erdal
0000-0003-4469-3438
Türkiye

Yayımlanma Tarihi

1 Mart 2026

Gönderilme Tarihi

14 Kasım 2025

Kabul Tarihi

4 Şubat 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: 2026

DOI

https://doi.org/10.35229/jaes.1823857

IZ

https://izlik.org/JA35FG99BB

APA

Şensoy, E., Güneş, E., & Erdal, M. O. (2026). Analyzing the Impact of Ankaferd Blood Stopper on the Gastrointestinal Tract of Rats Using Histological Techniques. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1823857

JAES, 2016- 11.yıl

Analyzing the Impact of Ankaferd Blood Stopper on the Gastrointestinal Tract of Rats Using Histological Techniques

Abstract

Keywords

Ankaferd Kan Durdurucu'nun Sıçanların Gastrointestinal Sistemi Üzerindeki Etkisinin Histolojik Teknikler Kullanılarak İncelenmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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