Analyzing the Impact of Ankaferd Blood Stopper on the Gastrointestinal Tract of Rats Using Histological Techniques
Öz
Ankaferd Blood Stopper is a medicine gained from five types of plants used in traditional Turkish medicine. It can stop different types of bleeding in a short time. The study aims to determine the impact of ABS on the gastrointestinal system and to test its reliability. Four groups were created with Wistar albino rats. (Control, ABS local application, ABS-doped wound dressing, ABS non-doped wound dressing, n:6). A 15x15 millimeter wound was formed on the back of each rat, and the healing processes were closely followed. The investigation was terminated by sacrificing the rats by cervical dislocation on the 14th day. Stomach and small intestine tissues pulled out from mice were moved to 10% formaldehyde before the xylene and alcohol series. After routine histological methods, the tissues were dyed with hematoxylin-eosin and examined under light and electron microscopy. Mean stomach (p: 0.01) and small intestine weights (p: 0.02) increased in ABS-applied groups. The histopathological structure of the stomach tissue was normal in the ABS-applied groups. The profile of the tunica muscularis and submucosa were regular, and there was no degeneration. No atrophy or rupture was observed in the villi. Because ABS does not reason lesions in the stomach and small intestine, ABS may be a safe haemostatic agent option in the gastrointestinal tract. Further investigation is required to evaluate the impacts of prolonged ABS usage.
Anahtar Kelimeler
Ankaferd Blood Stopper, gastrointestinal system, histopathological effect, rat
Destekleyen Kurum
Karamanoğlu Mehmetbey Üniversitesi Bilimsel Araştırma Projeleri Komisyonu.
Proje Numarası
03-M-21
Etik Beyan
Selçuk Üniversitesi Deneysel Tıp Araştırma ve Uygulama Merkezi'nden etik kurul onayı alınmıştır (SUDAM-2019-2/25.01.2019)
Teşekkür
Bu araştırma, Karamanoğlu Mehmetbey Üniversitesi Bilimsel Araştırma Projeleri Komisyonu tarafından kabul edilen 03-M-21 numaralı proje kapsamında desteklenmiştir.
Kaynakça
- ABS. (Ankaferd Blood Stopper) (2025)). Absorbable wet tampon. Retrieved from https://ankaferd.com/en/product/ankaferd- bloodstopper-absorbable-wet-tampon (Access date: November 6, 2025).
- Akbal, E., Köklü, S., Astarcı, H. M., Koçak, E., Karaca, G., Beyazıt, Y., & Haznedaroğlu, İ. (2013). The effects of oral high-dose Ankaferd administration on the gastrointestinal system. International Journal of Medical Sciences, 10(4), 451. https://doi.org/10.7150/ijms.4875
- Aksu, S.İ., & Deprem, T. (2021). Immunohistochemical localization of hepatocyte growth factor (HGF) in the fundus region of the stomach of diabetic and non-diabetic mice. Atatürk University Journal of Veterinary Sciences, 16(3), 251-258. https://doi.org/10.17094/ataunivbd.879099
- Aktop, S., Emekli-Alturfan, E., Özer, C., Gonul, O., Garip, H., Yarat, A., & Göker, K. (2014). Effects of Ankaferd Blood Stopper and Celox on the tissue factor activities of warfarin-treated rats. Clinical & Applied Thrombosis/Hemostasis, 20(1), 16-21. https://doi.org/10.1177/1076029613490254
- Ambekar, R.S., & Kandasubramanian, B. (2019). Advancements in nanofibers for wound dressing: A review. European Polymer Journal, 117, 304- 336. https://doi.org/10.1016/j.eurpolymj.2019.05.020
- Atzori, L., Lucattelli, M., & Scotton, C.J. (2009). Absence of proteinase-activated receptor-1 signaling in mice confers protection from fMLP- induced goblet cell metaplasia. American Journal of Respiratory Cell and Molecular Biology, 41(6), 680-687. https://doi.org/10.1165/rcmb.2007- 0386OC
- Behçet, A.L., Kılıç, H., Zengin, S., Güler, M., Taysi, S., Yıldırım, C., & Sarı, İ. (2014). Efficiency of Ankaferd Blood Stopper used in bleeding control on intraabdominal adhesions formed postoperatively. Clinical & Applied Thrombosis/Hemostasis, 20(5), 524-529. https://doi.org/10.1177/10760296124717
- Beyazit, Y., Kurt, M., & Kekilli, M. (2010). Evaluation of the hemostatic effects of Ankaferd as an alternative medicine. Alternative Medicine Review, 15(4), 329-336.
- Bilgili, H., Captug, O., & Kosar, A. (2010). Oral systemic administration of Ankaferd anticoagulant had no short-term toxicity in an in vivo rabbit experimental model. Clinical & Applied Thrombosis/Hemostasis, 16(6), 533-536. https://doi.org/10.1177/1076029609335912
- Bulut, E., Baş, B., Altunkaynak, B.Z., Bekçioğlu, B., Erdem Koç, G., Gönülol, E., Önger, M.E., & Kaplan, S. (2014). Efficacy of Ankaferd Blood Stopper on bone healing in diabetic rats: A stereological and histopathological study. Biotechnic & Histochemistry, 89(7), 535-543. https://doi.org/10.3109/10520295.2014.906657