Exploratory Proteomic Analysis of Rhododendron ponticum Bee Pollen Based on High-Resolution Mass Spectrometry
Öz
Bee pollen is a bee product that is widely preferred by people for its nutritional properties as well as its health-protective properties, and is defined as a ‘superfood’. In this preliminary exploratory study, monofloral Rhododendron ponticum bee pollen was analyzed using a label-free LC-QTOF-MS proteomic approach. According to the results obtained, a total of 37 proteins from the rhododendron plant have been fully confirmed in the pollen sample. Normalized spectrum counting values (NSAF) providing information about the semi-quantitative amount of these proteins were determined to be between 120.84 and 43259.22. Based on these findings, it can be said that the proteomic approach can provide biomarkers for monitoring the botanical source and quality of monofloral bee pollen. However, since no biological or technical replicate study was conducted, the findings obtained are exploratory in nature and should be considered semi-quantitative. To our knowledge, this study is the first to report the protein profile of R. ponticum bee pollen using a proteomic approach.
Anahtar Kelimeler
Etik Beyan
Kaynakça
- Adaškevičiūtė, V., Kaškonienė, V., Kaškonas, P., Barčauskaitė, K., & Maruška, A. (2019). Comparison of physicochemical properties of bee pollen with other bee products. Biomolecules, 9(12), 819.
- Afzaal, M., Saeed, F., Hussain, M., Shahid, F., Siddeeg, A., & Al-Farga, A. (2022). Proteomics as a promising biomarker in food authentication, quality and safety: A review. Food Science & Nutrition, 10(7), 2333–2346.
- Baky, M. H., Abouelela, M. B., Wang, K., & Farag, M. A. (2023). Bee pollen and bread as a super-food: A comparative review of their metabolome composition and quality assessment in the context of best recovery conditions. Molecules, 28(2), 715.
- Barsnes, H., & Vaudel, M. (2018). SearchGUI: A highly adaptable common interface for proteomics search and de novo engines. Journal of Proteome Research, 17(7), 2552–2555.
- Biundo, G., Calligaris, M., Pinto, M. L., D’Apolito, D., Pasqua, S., Vitale, G., ... & Scilabra, S. D. (2024). High-resolution proteomics and machine-learning identify protein classifiers of honey made by Sicilian black honeybees (Apis mellifera ssp. sicula). Food Research International, 194, 114872.
- Borutinskaitė, V., Treigytė, G., Matuzevičius, D., Zaikova, I., Čeksterytė, V., Navakauskas, D., ... & Navakauskienė, R. (2017). Proteomic analysis of pollen and blossom honey from rapeseed (Brassica napus L.). Journal of Apicultural Science, 61(1), 73–92.
- Čeksterytė, V., Treigytė, G., Matuzevičius, D., Jaškūnė, K., Navakauskas, D., Kurtinaitienė, B., & Navakauskienė, R. (2021). Comparative proteomic profile in bee- and manually-collected Taraxacum officinale pollen. Journal of Apicultural Research, 61(4), 543–556.
- Ecem Bayram, N. (2021). Vitamin, mineral, polyphenol, amino acid profile of bee pollen from Rhododendron ponticum (source of “mad honey”): nutritional and palynological approach. Journal of Food Measurement and Characterization, 15(3), 2659-2666.
- Giampieri, F., Quiles, J. L., Cianciosi, D., Forbes-Hernández, T. Y., Orantes-Bermejo, F. J., Alvarez-Suarez, J. M., & Battino, M. (2022). Bee products: An emblematic example of underutilized sources of bioactive compounds. Journal of Agricultural and Food Chemistry, 70(23), 6833–6848.
- Halici, H., Un, H., Celik, S., Karakoy, Z., Bayraktutan, Z., Ozlu, C., ... & Mercantepe, F. (2025). Low-dose bee venom as a potential therapeutic agent against human chronic myeloid leukaemia cells. The Protein Journal, 44(3), 297–307.