Bioutilization of Cheese Whey and Corn Steep Liqour by Heterotrophic Microalgae Crypthecodinium cohnii for Biomass and Lipid Production

Yıl 2017, Cilt: 15 Sayı: 3, 233 - 241, 22.10.2017

Müge Isleten-hosoglu , Murat Elibol

https://doi.org/10.24323/akademik-gida.345256

Cited By: 2

Öz

Many
different wastewater and by-products derived from industrial activities
potentially support microalgal growth by providing a cost-effective and
sustainable solutions. In this present study, it
was aimed to biologically utilize cheese whey (CW) and corn steep liquor (CLS)
for microalgal biomass and lipid production by using these wastes in culture
media for heterotrophic microalga Crypthecodinium
cohnii cultivation. To determine nutrient
requirements for C. cohnii growing in
a medium prepared with CSL and in CW, statistical screening tools were used. CSL significantly enhanced microalgal growth
and it could be an alternative to yeast extract as the primary nutrient source.
As for CW, it served as a good culture medium for C. cohnii with the supplement of some of nutrients and eliminated the need for fresh water. Thus, a
new culture medium was developed by combining undiluted CW and CSL and optimized for the growth of
C. cohnii. Lastly, in a scale-up
attempt by using this new medium, microalgal production was performed in a 3 L
stirred tank bioreactor. C. cohnii
yielded relatively high biomass productivity (2.28 g/L.d) and lipid content
(28.7% dry weight) in the optimized medium. Althoug C. cohnii was known for its ability to accumulate high amounts of
docosahexaenoic acid (DHA), it transformed its fatty acid composition to an increased
proportion of saturated and monounsaturated fatty acids (C16:0-C18:1) that comprise
~70% of total fatty acids (TFA) when it was
cultivated in CW mainly supplemented with CSL. Thus, C. cohnii seemed to be
more feasible for biodiesel production than any other purposes when it was
cultivated in this new medium.

Anahtar Kelimeler

Crypthecodinium cohnii, Corn steep liqour, Cheese whey, Optimization, Lipid

Peyniraltı Suyu ve Mısır Islatma Şurubunun Heterotrofik Mikroalg Crypthecodinium cohnii ile Biyokütle ve Yağ Üretimi Amacıyla Biyolojik Olarak Değerlendirilmesi

Öz

Endüstriyel faaliyetlerden elde edilen birçok
farklı atık su ve yan
ürünler maliyet-etkin ve sürdürülebilir
mikroalg kültüvasyonu için potansiyel
kaynaklardır. Mevcut çalışmada,
heterotrofik mikroalg Crypthecodinium cohnii kültüvasyonu için kültür besiyerinde peynir altı suyu
(CW) ve mısır ıslatma şurubu
(CSL) kullanılarak, bu atıkların mikroalgal biyokütle ve yağ üretimi amacıyla biyolojik
olarak değerlendirilmesi hedeflenmiştir. Organizmanın, her iki ortamdaki
(CW ve CSL) besin ihtiyaçlarını belirlemek amacıyla istatistiksel tarama
metodları kullanılmıştır. CSL’nin C.
cohnii biyokütle gelişimini olumlu yönde desteklediği ve organizmanın
kültür ortamında kullanılan maya ekstraktının alternatifi olarak
kullanılabileceği belirlenmiştir. Peyniraltı suyunun ise, organizma için
gerekli mineraller açısından önemli bir kaynak olduğu ve CW’in doğrudan
kullanımıyla kültür ortamındaki su ihtiyacını karşılama avantajına sahip olduğu
sonucuna varılmıştır. Bu şekilde
C. cohnii
kültüvasyonunda kullanılacak yeni

kültür ortamı seyreltilmemiş CW ve
CSL’nin birlikte kullanımı ile
geliştirilmiştir ve
optimizasyonu yapılmıştır.
Son olarak, yapılan ölçek
büyütme çalışmalarında
yeni kültür ortamı kullanılarak
3 L’lik karıştırmalı tank biyoreaktörde üretimler gerçekleştirilmiştir.
Optimizasyonu yapılan kültür ortamında kısmen yüksek biyokütle verimliliği
(2.28 g/L.gün) ve yağ oranı (% 28.7 kuru ağırlık) sağlanmıştır. C. cohnii biyokütlede son derece
yüksek oranlarda dokozahekzanoik asit (DHA) üreticisi bir tür olarak
bilinmesine rağmen, CSL ile
zenginleştirilmiş CW ortamında biyokütlesindeki
yağ asidi kompozisyonunu değiştirerek, daha çok tekli doymuş ve tekli doymamış
yağ asitlerincee (C16:0-C18:1) zengin bir yağ profiline (toplam yağ asidi
kompozisyonunun ~70%’i) sahip olduğu saptanmıştır.  Buna göre, endüstriyel atıkların
değerlendirildiği bu kültür ortamında yetiştirilen C. cohnii’nin biyodizel üretimi için uygun bir kaynak olabileceği
sonucuna varılmıştır.

Anahtar Kelimeler

Crypthecodinium cohnii, Mısır ıslatma şurubu, Peynir altı suyu, Optimizasyon, Yağ

Kaynakça

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